Expression pattern of DKK3, dickkopf WNT signaling pathway inhibitor 3, in the malignant progression of oral submucous fibrosis

  • Authors:
    • Shanghui Zhou
    • Yun Zhu
    • Mubarak Mashrah
    • Xinyu Zhang
    • Zhijing He
    • Zhigang Yao
    • Chunye Zhang
    • Feng Guo
    • Yongjie Hu
    • Chenping Zhang
  • View Affiliations

  • Published online on: December 8, 2016     https://doi.org/10.3892/or.2016.5307
  • Pages: 979-985
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Abstract

Oral squamous cell carcinoma (OSCC) is a common malignant neoplasm of the head and neck. Oral submucous fibrosis (OSF) is one of its pre-cancerous lesions; however, the key molecular events in the tumorigenesis of OSF remain elusive. Dickkopf WNT signaling pathway inhibitor 3 (DKK3) is one of the Wnt antagonists, and its downregulation and methylation have been reported in multiple malignancies, while no report of its expression in the carcinogenesis of OSF exists. In the present study, we investigated DKK3 expression at the protein and mRNA levels by immunochemical staining and semi‑quantitative RT-PCR in normal oral, OSF and OSCC tissues. We found that DKK3 was readily expressed in normal oral mucous tissues, but was gradually increased in early, moderately advanced and advanced OSF tissues, and strongly expressed in OSCC tissues. DKK3 was localized in the cytoplasm during OSF progression. A rare mutation of DKK3 was observed in OSCC, along with increased copy numbers. Furthermore, through analysis of its co-expressed genes, DDK3 may deregulate Wnt signaling, p53 signaling, apoptosis, Ca2+ signaling and mitochondrial signaling pathways in OSCC pathogenesis. Thus, our results demonstrated that DKK3 is upregulated in the carcinogenesis of OSF, due to gain of copy number, which could be a potential tumor marker for the early detection of OSCC.
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February-2017
Volume 37 Issue 2

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Zhou S, Zhu Y, Mashrah M, Zhang X, He Z, Yao Z, Zhang C, Guo F, Hu Y, Zhang C, Zhang C, et al: Expression pattern of DKK3, dickkopf WNT signaling pathway inhibitor 3, in the malignant progression of oral submucous fibrosis. Oncol Rep 37: 979-985, 2017
APA
Zhou, S., Zhu, Y., Mashrah, M., Zhang, X., He, Z., Yao, Z. ... Zhang, C. (2017). Expression pattern of DKK3, dickkopf WNT signaling pathway inhibitor 3, in the malignant progression of oral submucous fibrosis. Oncology Reports, 37, 979-985. https://doi.org/10.3892/or.2016.5307
MLA
Zhou, S., Zhu, Y., Mashrah, M., Zhang, X., He, Z., Yao, Z., Zhang, C., Guo, F., Hu, Y., Zhang, C."Expression pattern of DKK3, dickkopf WNT signaling pathway inhibitor 3, in the malignant progression of oral submucous fibrosis". Oncology Reports 37.2 (2017): 979-985.
Chicago
Zhou, S., Zhu, Y., Mashrah, M., Zhang, X., He, Z., Yao, Z., Zhang, C., Guo, F., Hu, Y., Zhang, C."Expression pattern of DKK3, dickkopf WNT signaling pathway inhibitor 3, in the malignant progression of oral submucous fibrosis". Oncology Reports 37, no. 2 (2017): 979-985. https://doi.org/10.3892/or.2016.5307