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Article Open Access

Fulvestrant reverses doxorubicin resistance in multidrug-resistant breast cell lines independent of estrogen receptor expression

  • Authors:
    • Yuan Huang
    • Donghai Jiang
    • Meihua Sui
    • Xiaojia Wang
    • Weimin Fan
  • View Affiliations / Copyright

    Affiliations: Department of Chemotherapy, Zhejiang Cancer Hospital, Hangzhou, Zhejiang 310022, P.R. China, Program of Innovative Cancer Therapeutics, Key Laboratory of Combined Multi-Organ Transplantation, Ministry of Public Health, First Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, Zhejiang 310006, P.R. China, Center for Cancer Biology and Innovative Therapeutics, Clinical Research Institute, Zhejiang Provincial People's Hospital, Hangzhou, Zhejiang 310014, P.R. China
    Copyright: © Huang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 705-712
    |
    Published online on: December 14, 2016
       https://doi.org/10.3892/or.2016.5315
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Abstract

Drug resistance, a major obstacle to successful cancer chemotherapy, frequently occurs in recurrent or metastatic breast cancer and results in poor clinical response. Fulvestrant is a new type of selective estrogen receptor (ER) downregulator and a promising endocrine therapy for breast cancer. In this study, we evaluated the combination treatment of fulvestrant and doxorubicin in ER-negative multidrug-resistant (MDR) breast cancer cell lines Bads‑200 and Bats‑72. Fulvestrant potentiated doxorubicin-induced cytotoxicity, apoptosis and G2/M arrest with upregulation of cyclin B1. It functioned as a substrate for P-glycoprotein (P-gp) without affecting its expression. Furthermore, fulvestrant not only restored the intracellular accumulation of doxorubicin but also relocalized it to the nuclei in Bats‑72 and Bads‑200 cells, which may be another potential mechanism of reversal of P-gp mediated doxorubicin resistance. These results indicated that the combination of fulvestrant and doxorubicin-based chemotherapy may be feasible and effective for patients with advanced breast cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Huang Y, Jiang D, Sui M, Wang X and Fan W: Fulvestrant reverses doxorubicin resistance in multidrug-resistant breast cell lines independent of estrogen receptor expression. Oncol Rep 37: 705-712, 2017.
APA
Huang, Y., Jiang, D., Sui, M., Wang, X., & Fan, W. (2017). Fulvestrant reverses doxorubicin resistance in multidrug-resistant breast cell lines independent of estrogen receptor expression. Oncology Reports, 37, 705-712. https://doi.org/10.3892/or.2016.5315
MLA
Huang, Y., Jiang, D., Sui, M., Wang, X., Fan, W."Fulvestrant reverses doxorubicin resistance in multidrug-resistant breast cell lines independent of estrogen receptor expression". Oncology Reports 37.2 (2017): 705-712.
Chicago
Huang, Y., Jiang, D., Sui, M., Wang, X., Fan, W."Fulvestrant reverses doxorubicin resistance in multidrug-resistant breast cell lines independent of estrogen receptor expression". Oncology Reports 37, no. 2 (2017): 705-712. https://doi.org/10.3892/or.2016.5315
Copy and paste a formatted citation
x
Spandidos Publications style
Huang Y, Jiang D, Sui M, Wang X and Fan W: Fulvestrant reverses doxorubicin resistance in multidrug-resistant breast cell lines independent of estrogen receptor expression. Oncol Rep 37: 705-712, 2017.
APA
Huang, Y., Jiang, D., Sui, M., Wang, X., & Fan, W. (2017). Fulvestrant reverses doxorubicin resistance in multidrug-resistant breast cell lines independent of estrogen receptor expression. Oncology Reports, 37, 705-712. https://doi.org/10.3892/or.2016.5315
MLA
Huang, Y., Jiang, D., Sui, M., Wang, X., Fan, W."Fulvestrant reverses doxorubicin resistance in multidrug-resistant breast cell lines independent of estrogen receptor expression". Oncology Reports 37.2 (2017): 705-712.
Chicago
Huang, Y., Jiang, D., Sui, M., Wang, X., Fan, W."Fulvestrant reverses doxorubicin resistance in multidrug-resistant breast cell lines independent of estrogen receptor expression". Oncology Reports 37, no. 2 (2017): 705-712. https://doi.org/10.3892/or.2016.5315
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