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Article

HELQ reverses the malignant phenotype of osteosarcoma cells via CHK1-RAD51 signaling pathway

  • Authors:
    • Dong Ning Liu
    • Yun Fei Zhou
    • Ai Fen Peng
    • Xin Hua Long
    • Xuan Yin Chen
    • Zhi Li Liu
    • Hong Xia
  • View Affiliations / Copyright

    Affiliations: Southern Medical University, Guangzhou, Guangdong, P.R. China, Department of Orthopedics, The First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi, P.R. China, Jiangxi University of Traditional Chinese Medicine, Nanchang, Jiangxi, P.R. China
  • Pages: 1107-1113
    |
    Published online on: December 20, 2016
       https://doi.org/10.3892/or.2016.5329
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Abstract

HELQ is a DNA helicase important for repair of DNA lesions and has been linked to several types of cancer. However, little is known about its relationship with osteosarcoma (OS) and its mechanism. In the present study, the expression of HELQ and its downstream mediators in OS cells was assayed by quantitative PCR and western blot analysis. The function of HELQ in OS cells was investigated by Transwell invasion, wound healing, CCK8 assays and Comet assay. The results demonstrated that HELQ gene and protein were expressed in OS cells. OS cell invasion, migration, proliferation and DNA damage repair were enhanced by HELQ knock-down with shRNA-lentivirus and inhibited by HELQ overexpression with lentivirus transfection. Furthermore, the antitumor activities of HELQ may be associated with upregulated expression of the DNA damage-related proteins CHK1 and RAD51. Our findings indicated that HELQ confers an anti-invasive phenotype on OS cells by activating the CHK1-RAD51 signaling pathway and suggested that HELQ could be recognized as a promising therapeutic target for OS and other types of malignant tumors.
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Copy and paste a formatted citation
Spandidos Publications style
Liu DN, Zhou YF, Peng AF, Long XH, Chen XY, Liu ZL and Xia H: HELQ reverses the malignant phenotype of osteosarcoma cells via CHK1-RAD51 signaling pathway. Oncol Rep 37: 1107-1113, 2017.
APA
Liu, D.N., Zhou, Y.F., Peng, A.F., Long, X.H., Chen, X.Y., Liu, Z.L., & Xia, H. (2017). HELQ reverses the malignant phenotype of osteosarcoma cells via CHK1-RAD51 signaling pathway. Oncology Reports, 37, 1107-1113. https://doi.org/10.3892/or.2016.5329
MLA
Liu, D. N., Zhou, Y. F., Peng, A. F., Long, X. H., Chen, X. Y., Liu, Z. L., Xia, H."HELQ reverses the malignant phenotype of osteosarcoma cells via CHK1-RAD51 signaling pathway". Oncology Reports 37.2 (2017): 1107-1113.
Chicago
Liu, D. N., Zhou, Y. F., Peng, A. F., Long, X. H., Chen, X. Y., Liu, Z. L., Xia, H."HELQ reverses the malignant phenotype of osteosarcoma cells via CHK1-RAD51 signaling pathway". Oncology Reports 37, no. 2 (2017): 1107-1113. https://doi.org/10.3892/or.2016.5329
Copy and paste a formatted citation
x
Spandidos Publications style
Liu DN, Zhou YF, Peng AF, Long XH, Chen XY, Liu ZL and Xia H: HELQ reverses the malignant phenotype of osteosarcoma cells via CHK1-RAD51 signaling pathway. Oncol Rep 37: 1107-1113, 2017.
APA
Liu, D.N., Zhou, Y.F., Peng, A.F., Long, X.H., Chen, X.Y., Liu, Z.L., & Xia, H. (2017). HELQ reverses the malignant phenotype of osteosarcoma cells via CHK1-RAD51 signaling pathway. Oncology Reports, 37, 1107-1113. https://doi.org/10.3892/or.2016.5329
MLA
Liu, D. N., Zhou, Y. F., Peng, A. F., Long, X. H., Chen, X. Y., Liu, Z. L., Xia, H."HELQ reverses the malignant phenotype of osteosarcoma cells via CHK1-RAD51 signaling pathway". Oncology Reports 37.2 (2017): 1107-1113.
Chicago
Liu, D. N., Zhou, Y. F., Peng, A. F., Long, X. H., Chen, X. Y., Liu, Z. L., Xia, H."HELQ reverses the malignant phenotype of osteosarcoma cells via CHK1-RAD51 signaling pathway". Oncology Reports 37, no. 2 (2017): 1107-1113. https://doi.org/10.3892/or.2016.5329
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