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Article

Suppression of PTEN/AKT signaling decreases the expression of TUBB3 and TOP2A with subsequent inhibition of cell growth and induction of apoptosis in human breast cancer MCF-7 cells via ATP and caspase-3 signaling pathways

  • Authors:
    • Zhenhua Yang
    • Ying Liu
    • Changzheng Shi
    • Yuqin Zhang
    • Rongzhao Lv
    • Rong Zhang
    • Qian Wang
    • Yiming Wang
  • View Affiliations / Copyright

    Affiliations: Department of Oncology, The First Affiliated Hospital of Jinan University, Guangzhou 510630, P.R. China, Medical Imaging Center, The First Affiliated Hospital of Jinan University, Guangzhou 510630, P.R. China, Department of Surgery on Galactophore, The First Affiliated Hospital of Jinan University, Guangzhou 510630, P.R. China
  • Pages: 1011-1019
    |
    Published online on: January 9, 2017
       https://doi.org/10.3892/or.2017.5358
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Abstract

The aim of the present study was to evaluate the effects of PTEN/AKT signaling on TUBB3 and TOP2A expression and on the subsequent cell growth of human breast cancer MCF-7 cells. We found that the disease-free survival (DFS) and overall survival (OS) of breast cancer patients with TUBB3‑positive tumors were lower than these rates in the patients with TUBB3-negative tumors. Meanwhile, DFS and OS of breast cancer patients with TOP2A-positive tumors were also lower than these rates in patients with TOP2A-negative tumors. Suppression of PTEN reduced the protein expression of TUBB3 and TOP2A in MCF-7 cells. Suppression of PTEN also reduced cell proliferation and induced apoptosis and caspase-3 activity in MCF-7 cells. Moreover, an increase in ATP also reduced TUBB3 and TOP2A protein expression, reduced cell proliferation and induced apoptosis and caspase-3 activity in the MCF-7 cells following suppression of PTEN. Suppression of phosphorylation-AKT (p-AKT) reduced the protein expression of TUBB3 and TOP2A in the MCF-7 cells. Suppression of p-AKT also reduced cell proliferation and induced apoptosis and caspase-3 activity in the MCF-7 cells. Then, ATP also reduced TUBB3 and TOP2A protein expression, reduced cell proliferation and induced apoptosis and caspase-3 activity in MCF-7 cells following suppression of p-AKT. These results suggest that PTEN/AKT signaling affects the expression of TUBB3 and TOP2A reducing cell growth and inducing apoptosis of human breast cancer MCF-7 cells through ATP and caspase-3 signaling pathways. TUBB3 and TOP2A may be promising prognostic markers for the efficacy of adjuvant cisplatin-based chemotherapy.
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Copy and paste a formatted citation
Spandidos Publications style
Yang Z, Liu Y, Shi C, Zhang Y, Lv R, Zhang R, Wang Q and Wang Y: Suppression of PTEN/AKT signaling decreases the expression of TUBB3 and TOP2A with subsequent inhibition of cell growth and induction of apoptosis in human breast cancer MCF-7 cells via ATP and caspase-3 signaling pathways. Oncol Rep 37: 1011-1019, 2017.
APA
Yang, Z., Liu, Y., Shi, C., Zhang, Y., Lv, R., Zhang, R. ... Wang, Y. (2017). Suppression of PTEN/AKT signaling decreases the expression of TUBB3 and TOP2A with subsequent inhibition of cell growth and induction of apoptosis in human breast cancer MCF-7 cells via ATP and caspase-3 signaling pathways. Oncology Reports, 37, 1011-1019. https://doi.org/10.3892/or.2017.5358
MLA
Yang, Z., Liu, Y., Shi, C., Zhang, Y., Lv, R., Zhang, R., Wang, Q., Wang, Y."Suppression of PTEN/AKT signaling decreases the expression of TUBB3 and TOP2A with subsequent inhibition of cell growth and induction of apoptosis in human breast cancer MCF-7 cells via ATP and caspase-3 signaling pathways". Oncology Reports 37.2 (2017): 1011-1019.
Chicago
Yang, Z., Liu, Y., Shi, C., Zhang, Y., Lv, R., Zhang, R., Wang, Q., Wang, Y."Suppression of PTEN/AKT signaling decreases the expression of TUBB3 and TOP2A with subsequent inhibition of cell growth and induction of apoptosis in human breast cancer MCF-7 cells via ATP and caspase-3 signaling pathways". Oncology Reports 37, no. 2 (2017): 1011-1019. https://doi.org/10.3892/or.2017.5358
Copy and paste a formatted citation
x
Spandidos Publications style
Yang Z, Liu Y, Shi C, Zhang Y, Lv R, Zhang R, Wang Q and Wang Y: Suppression of PTEN/AKT signaling decreases the expression of TUBB3 and TOP2A with subsequent inhibition of cell growth and induction of apoptosis in human breast cancer MCF-7 cells via ATP and caspase-3 signaling pathways. Oncol Rep 37: 1011-1019, 2017.
APA
Yang, Z., Liu, Y., Shi, C., Zhang, Y., Lv, R., Zhang, R. ... Wang, Y. (2017). Suppression of PTEN/AKT signaling decreases the expression of TUBB3 and TOP2A with subsequent inhibition of cell growth and induction of apoptosis in human breast cancer MCF-7 cells via ATP and caspase-3 signaling pathways. Oncology Reports, 37, 1011-1019. https://doi.org/10.3892/or.2017.5358
MLA
Yang, Z., Liu, Y., Shi, C., Zhang, Y., Lv, R., Zhang, R., Wang, Q., Wang, Y."Suppression of PTEN/AKT signaling decreases the expression of TUBB3 and TOP2A with subsequent inhibition of cell growth and induction of apoptosis in human breast cancer MCF-7 cells via ATP and caspase-3 signaling pathways". Oncology Reports 37.2 (2017): 1011-1019.
Chicago
Yang, Z., Liu, Y., Shi, C., Zhang, Y., Lv, R., Zhang, R., Wang, Q., Wang, Y."Suppression of PTEN/AKT signaling decreases the expression of TUBB3 and TOP2A with subsequent inhibition of cell growth and induction of apoptosis in human breast cancer MCF-7 cells via ATP and caspase-3 signaling pathways". Oncology Reports 37, no. 2 (2017): 1011-1019. https://doi.org/10.3892/or.2017.5358
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