MicroRNA-187 promotes growth and metastasis of gastric cancer by inhibiting FOXA2

Li,Cong; Lu,Sumei; Shi,Yubin

doi:10.3892/or.2017.5370

MicroRNA-187 promotes growth and metastasis of gastric cancer by inhibiting FOXA2

Authors:
- Cong Li
- Sumei Lu
- Yubin Shi
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Affiliations: Department of Emergency Internal Medicine, Tongren Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, P.R. China, Department of Internal Neurology, Tongren Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, P.R. China
Published online on: January 16, 2017 https://doi.org/10.3892/or.2017.5370
Pages: 1747-1755

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Abstract

MicroRNAs (miRNAs) play an active role in the pathogenesis of gastric cancer. The expression and biological function for miR-187 in gastric cancer remains unknow. In the present study, we demonstrated that miR-187 expression was increased in gastric cancer (GC) tissues and cells. Increased expression level of miR-187 was associated with adverse clinical features including tumor size, lymph metastasis and TNM stage, and decreased overall survival and disease-free survival of GC patients. Functionally, overexpression miR-187 could promote while inhibition of miR-187 could suppress, the proliferation, migration and invasion of GC cells in vitro. In vivo experiments showed that overexpression of miR-187 promoted the growth and lung metastasis of SGC-7901 cells in nude mice. Mechanically, we confirmed that FOXA2 was the downstream target of miR-187 in GC cells using luciferase assay, qRT-PCR and western blot analysis. Moreover, overexpression of FOXA2 abrogated the promoting effects of miR-187 overexpression on SGC-7901 cell proliferation, migration and invasion, while inhibition of FOXA2 reversed the inhibitory effects of miR-187 downregulation on these biological functions of AGS cells, suggesting that FOXA2 was a functional mediator of miR-187 in GC. Therefore, this study indicates that miR-187 is potentially a biomarker and treatment target for GC patients.

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