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Article

MicroRNA-194 represses glioma cell epithelial‑to‑mesenchymal transition by targeting Bmi1

  • Authors:
    • Xi Zhang
    • Chunyan Wei
    • Jin Li
    • Jiali Liu
    • Jianqiang Qu
  • View Affiliations / Copyright

    Affiliations: Department of Neurosurgery, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710004, P.R. China, Department of Gynaecology and Obstetrics, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710004, P.R. China, Clinical Laboratory, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710004, P.R. China
  • Pages: 1593-1600
    |
    Published online on: January 17, 2017
       https://doi.org/10.3892/or.2017.5376
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Abstract

MicroRNA-194 (miR-194) is frequently dysregulated in many types of cancer. However, the function of miR-194 in glioma remains unknown. In the present study, we aimed to investigate the biological functions of miR-194 in glioma and the potential molecular mechanism of miR-194 involved in glioma progression. We found that miR-194 expression was significantly reduced in glioma specimens and cell lines, as detected by real-time quantitative polymerase chain reaction (RT-qPCR) analysis. The overexpression of miR-194 inhibited while the suppression of miR-194 promoted cell migration, invasion and epithelial mesenchymal transition (EMT) in glioma cells. Bioinformatics analysis showed that the B cell-specific moloney murine leukemia virus insertion site 1 (Bmi1) was a direct target of miR-194, which was validated by dual-luciferase reporter assay, RT-qPCR and western blot analysis. The restoration of Bmi1 expression significantly abrogated the suppressive effect of miR-194 on glioma cell EMT. Taken together, the present study suggests that miR-194 inhibits glioma cell EMT by targeting Bmi1 providing novel insights into understanding the pathogenesis of glioma. The restoration of miR-194 may be a potential therapeutic strategy for glioma treatment.
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Zhang X, Wei C, Li J, Liu J and Qu J: MicroRNA-194 represses glioma cell epithelial‑to‑mesenchymal transition by targeting Bmi1. Oncol Rep 37: 1593-1600, 2017.
APA
Zhang, X., Wei, C., Li, J., Liu, J., & Qu, J. (2017). MicroRNA-194 represses glioma cell epithelial‑to‑mesenchymal transition by targeting Bmi1. Oncology Reports, 37, 1593-1600. https://doi.org/10.3892/or.2017.5376
MLA
Zhang, X., Wei, C., Li, J., Liu, J., Qu, J."MicroRNA-194 represses glioma cell epithelial‑to‑mesenchymal transition by targeting Bmi1". Oncology Reports 37.3 (2017): 1593-1600.
Chicago
Zhang, X., Wei, C., Li, J., Liu, J., Qu, J."MicroRNA-194 represses glioma cell epithelial‑to‑mesenchymal transition by targeting Bmi1". Oncology Reports 37, no. 3 (2017): 1593-1600. https://doi.org/10.3892/or.2017.5376
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang X, Wei C, Li J, Liu J and Qu J: MicroRNA-194 represses glioma cell epithelial‑to‑mesenchymal transition by targeting Bmi1. Oncol Rep 37: 1593-1600, 2017.
APA
Zhang, X., Wei, C., Li, J., Liu, J., & Qu, J. (2017). MicroRNA-194 represses glioma cell epithelial‑to‑mesenchymal transition by targeting Bmi1. Oncology Reports, 37, 1593-1600. https://doi.org/10.3892/or.2017.5376
MLA
Zhang, X., Wei, C., Li, J., Liu, J., Qu, J."MicroRNA-194 represses glioma cell epithelial‑to‑mesenchymal transition by targeting Bmi1". Oncology Reports 37.3 (2017): 1593-1600.
Chicago
Zhang, X., Wei, C., Li, J., Liu, J., Qu, J."MicroRNA-194 represses glioma cell epithelial‑to‑mesenchymal transition by targeting Bmi1". Oncology Reports 37, no. 3 (2017): 1593-1600. https://doi.org/10.3892/or.2017.5376
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