MicroRNA-194 represses glioma cell epithelial‑to‑mesenchymal transition by targeting Bmi1

  • Authors:
    • Xi Zhang
    • Chunyan Wei
    • Jin Li
    • Jiali Liu
    • Jianqiang Qu
  • View Affiliations

  • Published online on: January 17, 2017     https://doi.org/10.3892/or.2017.5376
  • Pages: 1593-1600
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Abstract

MicroRNA-194 (miR-194) is frequently dysregulated in many types of cancer. However, the function of miR-194 in glioma remains unknown. In the present study, we aimed to investigate the biological functions of miR-194 in glioma and the potential molecular mechanism of miR-194 involved in glioma progression. We found that miR-194 expression was significantly reduced in glioma specimens and cell lines, as detected by real-time quantitative polymerase chain reaction (RT-qPCR) analysis. The overexpression of miR-194 inhibited while the suppression of miR-194 promoted cell migration, invasion and epithelial mesenchymal transition (EMT) in glioma cells. Bioinformatics analysis showed that the B cell-specific moloney murine leukemia virus insertion site 1 (Bmi1) was a direct target of miR-194, which was validated by dual-luciferase reporter assay, RT-qPCR and western blot analysis. The restoration of Bmi1 expression significantly abrogated the suppressive effect of miR-194 on glioma cell EMT. Taken together, the present study suggests that miR-194 inhibits glioma cell EMT by targeting Bmi1 providing novel insights into understanding the pathogenesis of glioma. The restoration of miR-194 may be a potential therapeutic strategy for glioma treatment.
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March-2017
Volume 37 Issue 3

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Zhang X, Wei C, Li J, Liu J and Qu J: MicroRNA-194 represses glioma cell epithelial‑to‑mesenchymal transition by targeting Bmi1. Oncol Rep 37: 1593-1600, 2017.
APA
Zhang, X., Wei, C., Li, J., Liu, J., & Qu, J. (2017). MicroRNA-194 represses glioma cell epithelial‑to‑mesenchymal transition by targeting Bmi1. Oncology Reports, 37, 1593-1600. https://doi.org/10.3892/or.2017.5376
MLA
Zhang, X., Wei, C., Li, J., Liu, J., Qu, J."MicroRNA-194 represses glioma cell epithelial‑to‑mesenchymal transition by targeting Bmi1". Oncology Reports 37.3 (2017): 1593-1600.
Chicago
Zhang, X., Wei, C., Li, J., Liu, J., Qu, J."MicroRNA-194 represses glioma cell epithelial‑to‑mesenchymal transition by targeting Bmi1". Oncology Reports 37, no. 3 (2017): 1593-1600. https://doi.org/10.3892/or.2017.5376