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Article

Downregulation of leucine‑rich‑α‑2‑glycoprotein 1 expression is associated with the tumorigenesis of head and neck squamous cell carcinoma

  • Authors:
    • Yongping Wang
    • Chen Chen
    • Qingquan Hua
    • Lei Wang
    • Fen Li
    • Man Li
    • Zhidan Mei
    • Tao Zhou
    • Bokui Xiao
    • Zezhang Tao
  • View Affiliations / Copyright

    Affiliations: Department of Otolaryngology-Head and Neck Surgery, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, P.R. China, Research Institute of Otolaryngology‑Head and Neck Surgery, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, P.R. China
  • Pages: 1503-1510
    |
    Published online on: January 17, 2017
       https://doi.org/10.3892/or.2017.5377
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Abstract

Leucine-rich-α-2-glycoprotein 1 (LRG1) is considered as a potential biomarker as it is aberrantly expressed in various malignancies. However, there is limited information regarding its role in head and neck squamous cell carcinoma (HNSCC). In the present study, we aimed to explore the expression pattern of LRG1 in HNSCC and its clinicopathological significance. We first analyzed LRG1 gene expression in HNSCC by investigating data obtained from the Gene Expression Omnibus (GEO) database. The results showed that LRG1 was downregulated in HNSCC tissues and its expression level was negatively related to tumor T and N stages and degree of malignancy. Then, we further tested a tissue microassay and clinical samples, respectively, by immunohistochemical staining and western blotting. Consistently, the results revealed that LRG1 expression was decreased in tumor tissues regardless of the grade of the tumor. Moreover, the protein level of LRG1 showed slight differences among four T stages or three N stages. In addition, there were no significant associations between LRG1 protein expression and other clinicopathological parameters such as gender, age, tumor location and clinical staging. These findings imply that downregulation of the expression of LRG1 is correlated with tumorigenesis but not with the development of HNSCC, indicating the potential clinical value of LRG1 in the early diagnosis of HNSCC.
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1 

Ota I, Okamoto N, Yane K, Takahashi A, Masui T, Hosoi H and Ohnishi T: Therapeutic strategies for head and neck cancer based on p53 status. Exp Ther Med. 3:585–591. 2012.PubMed/NCBI

2 

Ozdek A, Sarac S, Akyol MU, Unal OF and Sungur A: Histopathological predictors of occult lymph node metastases in supraglottic squamous cell carcinomas. Eur Arch Otorhinolaryngol. 257:389–392. 2000. View Article : Google Scholar : PubMed/NCBI

3 

Jemal A, Siegel R, Ward E, Hao Y, Xu J, Murray T and Thun MJ: Cancer statistics, 2008. CA Cancer J Clin. 58:71–96. 2008. View Article : Google Scholar : PubMed/NCBI

4 

Wang L, Chen C, Li F, Hua Q, Chen S, Xiao B, Dai M, Li M, Zheng A, Yu D, et al: Down-regulation of neutrophil gelatinase-associated lipocalin in head and neck squamous cell carcinoma correlated with tumorigenesis, not with metastasis. Int J Clin Exp Pathol. 8:8857–8868. 2015.PubMed/NCBI

5 

Koole K, van Kempen PM, Swartz JE, Peeters T, van Diest PJ, Koole R, van Es RJ and Willems SM: Fibroblast growth factor receptor 3 protein is overexpressed in oral and oropharyngeal squamous cell carcinoma. Cancer Med. 5:275–284. 2016. View Article : Google Scholar : PubMed/NCBI

6 

Haupt H and Baudner S: Isolation and characterization of an unknown, leucine-rich 3.1-S-alpha2-glycoprotein from human serum (author's transl). Hoppe Seylers Z Physiol Chem. 358:639–646. 1977.(In German). View Article : Google Scholar : PubMed/NCBI

7 

Kobe B and Kajava AV: The leucine-rich repeat as a protein recognition motif. Curr Opin Struct Biol. 11:725–732. 2001. View Article : Google Scholar : PubMed/NCBI

8 

Buchanan SG and Gay NJ: Structural and functional diversity in the leucine-rich repeat family of proteins. Prog Biophys Mol Biol. 65:1–44. 1996. View Article : Google Scholar : PubMed/NCBI

9 

Serada S, Fujimoto M, Terabe F, Iijima H, Shinzaki S, Matsuzaki S, Ohkawara T, Nezu R, Nakajima S, Kobayashi T, et al: Serum leucine-rich alpha-2 glycoprotein is a disease activity biomarker in ulcerative colitis. Inflamm Bowel Dis. 18:2169–2179. 2012. View Article : Google Scholar : PubMed/NCBI

10 

Kharbanda AB, Rai AJ, Cosme Y, Liu K and Dayan PS: Novel serum and urine markers for pediatric appendicitis. Acad Emerg Med. 19:56–62. 2012. View Article : Google Scholar : PubMed/NCBI

11 

Serada S, Fujimoto M, Ogata A, Terabe F, Hirano T, Iijima H, Shinzaki S, Nishikawa T, Ohkawara T, Iwahori K, et al: iTRAQ-based proteomic identification of leucine-rich alpha-2 glycoprotein as a novel inflammatory biomarker in autoimmune diseases. Ann Rheum Dis. 69:770–774. 2010. View Article : Google Scholar : PubMed/NCBI

12 

Wang X, Abraham S, McKenzie JA, Jeffs N, Swire M, Tripathi VB, Luhmann UF, Lange CA, Zhai Z, Arthur HM, et al: LRG1 promotes angiogenesis by modulating endothelial TGF-β signalling. Nature. 499:306–311. 2013. View Article : Google Scholar : PubMed/NCBI

13 

He X, Wang Y, Zhang W, Li H, Luo R, Zhou Y, Li C, Liao M, Huang H, Lv X, et al: Screening differential expression of serum proteins in AFP-negative HBV-related hepatocellular carcinoma using iTRAQ -MALDI-MS/MS. Neoplasma. Sep 20–2013.(Epub ahead of print). doi: 10.4149/neo_2014_001.

14 

Wu J, Xie X, Nie S, Buckanovich RJ and Lubman DM: Altered expression of sialylated glycoproteins in ovarian cancer sera using lectin-based ELISA assay and quantitative glycoproteomics analysis. J Proteome Res. 12:3342–3352. 2013. View Article : Google Scholar : PubMed/NCBI

15 

Wen SY, Zhang LN, Yang XM, Zhang YL, Ma L, Ge QL, Jiang SH, Zhu XL, Xu W, Ding WJ, et al: LRG1 is an independent prognostic factor for endometrial carcinoma. Tumour Biol. 35:7125–7133. 2014. View Article : Google Scholar : PubMed/NCBI

16 

Uen YH, Lin KY, Sun DP, Liao CC, Hsieh MS, Huang YK, Chen YW, Huang PH, Chen WJ, Tai CC, et al: Comparative proteomics, network analysis and post-translational modification identification reveal differential profiles of plasma Con A-bound glycoprotein biomarkers in gastric cancer. J Proteomics. 83:197–213. 2013. View Article : Google Scholar : PubMed/NCBI

17 

Wu RS, Yu CS, Liu KC, Huang HY, Ip SW, Lin JP, Chueh FS, Yang JS and Chung JG: Citosol (thiamylal sodium) triggers apoptosis and affects gene expressions of murine leukemia RAW 264.7 cells. Hum Exp Toxicol. 31:771–779. 2012. View Article : Google Scholar : PubMed/NCBI

18 

Ladd JJ, Busald T, Johnson MM, Zhang Q, Pitteri SJ, Wang H, Brenner DE, Lampe PD, Kucherlapati R, Feng Z, et al: Increased plasma levels of the APC-interacting protein MAPRE1, LRG1, and IGFBP2 preceding a diagnosis of colorectal cancer in women. Cancer Prev Res. 5:655–664. 2012. View Article : Google Scholar

19 

Kakisaka T, Kondo T, Okano T, Fujii K, Honda K, Endo M, Tsuchida A, Aoki T, Itoi T, Moriyasu F, et al: Plasma proteomics of pancreatic cancer patients by multi-dimensional liquid chromatography and two-dimensional difference gel electrophoresis (2D-DIGE): Up-regulation of leucine-rich alpha-2-glycoprotein in pancreatic cancer. J Chromatogr B Analyt Technol Biomed Life Sci. 852:257–267. 2007. View Article : Google Scholar : PubMed/NCBI

20 

Lindén M, Segersten U, Runeson M, Wester K, Busch C, Pettersson U, Lind SB and Malmström PU: Tumour expression of bladder cancer-associated urinary proteins. BJU Int. 112:407–415. 2013. View Article : Google Scholar : PubMed/NCBI

21 

Jemal A, Thun MJ, Ries LA, Howe HL, Weir HK, Center MM, Ward E, Wu XC, Eheman C, Anderson R, et al: Annual report to the nation on the status of cancer, 1975–2005, featuring trends in lung cancer, tobacco use, and tobacco control. J Natl Cancer Inst. 100:1672–1694. 2008. View Article : Google Scholar : PubMed/NCBI

22 

Wu J, Yin H, Zhu J, Buckanovich RJ, Thorpe JD, Dai J, Urban N and Lubman DM: Validation of LRG1 as a potential biomarker for detection of epithelial ovarian cancer by a blinded study. PLoS One. 10:e01211122015. View Article : Google Scholar : PubMed/NCBI

23 

Li Y, Zhang Y, Qiu F and Qiu Z: Proteomic identification of exosomal LRG1: A potential urinary biomarker for detecting NSCLC. Electrophoresis. 32:1976–1983. 2011. View Article : Google Scholar : PubMed/NCBI

24 

Lynch J, Meehan MH, Crean J, Copeland J, Stallings RL and Bray IM: Metastasis suppressor microRNA-335 targets the formin family of actin nucleators. PLoS One. 8:e784282013. View Article : Google Scholar : PubMed/NCBI

25 

Lynch J, Fay J, Meehan M, Bryan K, Watters KM, Murphy DM and Stallings RL: MiRNA-335 suppresses neuroblastoma cell invasiveness by direct targeting of multiple genes from the non-canonical TGF-β signalling pathway. Carcinogenesis. 33:976–985. 2012. View Article : Google Scholar : PubMed/NCBI

26 

Zhong D, He G, Zhao S, Li J, Lang Y, Ye W, Li Y, Jiang C and Li X: LRG1 modulates invasion and migration of glioma cell lines through TGF-β signaling pathway. Acta Histochem. 117:551–558. 2015. View Article : Google Scholar : PubMed/NCBI

27 

Zhang Y, Luo Q, Wang N, Hu F, Jin H, Ge T, Wang C and Qin W: LRG1 suppresses the migration and invasion of hepatocellular carcinoma cells. Med Oncol. 32:1462015. View Article : Google Scholar : PubMed/NCBI

28 

Takemoto N, Serada S, Fujimoto M, Honda H, Ohkawara T, Takahashi T, Nomura S, Inohara H and Naka T: Leucine-rich α-2-glycoprotein promotes TGFβ1-mediated growth suppression in the Lewis lung carcinoma cell lines. Oncotarget. 6:11009–11022. 2015. View Article : Google Scholar : PubMed/NCBI

29 

Schonberg DL, Lubelski D, Miller TE and Rich JN: Brain tumor stem cells: Molecular characteristics and their impact on therapy. Mol Aspects Med. 39:82–101. 2014. View Article : Google Scholar : PubMed/NCBI

30 

Cavazza A, Corradini C, Marini M, Roda LG and Valenti A: Capillary electrophoresis coupled with mass spectrometry for the evaluation of substance P enzymatic degradation by SaOS-2 human osteosarcoma. J Chromatogr B Analyt Technol Biomed Life Sci. 879:2501–2506. 2011. View Article : Google Scholar : PubMed/NCBI

31 

Sulzbacher I, Birner P, Dominkus M, Pichlhofer B and Mazal PR: Expression of platelet-derived growth factor-alpha receptor in human osteosarcoma is not a predictor of outcome. Pathology. 42:664–668. 2010. View Article : Google Scholar : PubMed/NCBI

32 

Zhong D, Zhao S, He G, Li J, Lang Y, Ye W, Li Y, Jiang C and Li X: Stable knockdown of LRG1 by RNA interference inhibits growth and promotes apoptosis of glioblastoma cells in vitro and in vivo. Tumour Biol. 36:4271–4278. 2015. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Wang Y, Chen C, Hua Q, Wang L, Li F, Li M, Mei Z, Zhou T, Xiao B, Tao Z, Tao Z, et al: Downregulation of leucine‑rich‑α‑2‑glycoprotein 1 expression is associated with the tumorigenesis of head and neck squamous cell carcinoma. Oncol Rep 37: 1503-1510, 2017.
APA
Wang, Y., Chen, C., Hua, Q., Wang, L., Li, F., Li, M. ... Tao, Z. (2017). Downregulation of leucine‑rich‑α‑2‑glycoprotein 1 expression is associated with the tumorigenesis of head and neck squamous cell carcinoma. Oncology Reports, 37, 1503-1510. https://doi.org/10.3892/or.2017.5377
MLA
Wang, Y., Chen, C., Hua, Q., Wang, L., Li, F., Li, M., Mei, Z., Zhou, T., Xiao, B., Tao, Z."Downregulation of leucine‑rich‑α‑2‑glycoprotein 1 expression is associated with the tumorigenesis of head and neck squamous cell carcinoma". Oncology Reports 37.3 (2017): 1503-1510.
Chicago
Wang, Y., Chen, C., Hua, Q., Wang, L., Li, F., Li, M., Mei, Z., Zhou, T., Xiao, B., Tao, Z."Downregulation of leucine‑rich‑α‑2‑glycoprotein 1 expression is associated with the tumorigenesis of head and neck squamous cell carcinoma". Oncology Reports 37, no. 3 (2017): 1503-1510. https://doi.org/10.3892/or.2017.5377
Copy and paste a formatted citation
x
Spandidos Publications style
Wang Y, Chen C, Hua Q, Wang L, Li F, Li M, Mei Z, Zhou T, Xiao B, Tao Z, Tao Z, et al: Downregulation of leucine‑rich‑α‑2‑glycoprotein 1 expression is associated with the tumorigenesis of head and neck squamous cell carcinoma. Oncol Rep 37: 1503-1510, 2017.
APA
Wang, Y., Chen, C., Hua, Q., Wang, L., Li, F., Li, M. ... Tao, Z. (2017). Downregulation of leucine‑rich‑α‑2‑glycoprotein 1 expression is associated with the tumorigenesis of head and neck squamous cell carcinoma. Oncology Reports, 37, 1503-1510. https://doi.org/10.3892/or.2017.5377
MLA
Wang, Y., Chen, C., Hua, Q., Wang, L., Li, F., Li, M., Mei, Z., Zhou, T., Xiao, B., Tao, Z."Downregulation of leucine‑rich‑α‑2‑glycoprotein 1 expression is associated with the tumorigenesis of head and neck squamous cell carcinoma". Oncology Reports 37.3 (2017): 1503-1510.
Chicago
Wang, Y., Chen, C., Hua, Q., Wang, L., Li, F., Li, M., Mei, Z., Zhou, T., Xiao, B., Tao, Z."Downregulation of leucine‑rich‑α‑2‑glycoprotein 1 expression is associated with the tumorigenesis of head and neck squamous cell carcinoma". Oncology Reports 37, no. 3 (2017): 1503-1510. https://doi.org/10.3892/or.2017.5377
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