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Article

Cripto-1 promotes epithelial-mesenchymal transition in prostate cancer via Wnt/β-catenin signaling

Corrigendum in: /10.3892/or.2024.8734
  • Authors:
    • Yan Liu
    • Zhenbang Qin
    • Kuo Yang
    • Ranlu Liu
    • Yong Xu
  • View Affiliations / Copyright

    Affiliations: Prostate Disease Laboratory, Tianjin Institute of Urology, Tianjin 300211, P.R. China
  • Pages: 1521-1528
    |
    Published online on: January 17, 2017
       https://doi.org/10.3892/or.2017.5378
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Abstract

The Cripto-1 (CR-1) derived EGF-CFC family was overexpressed in tumor development enhancing proliferation, epithelial-mesenchymal transition (EMT) and migration of tumor cells. However, correlation between CR-1 and prostate cancer (PCa) remains still unclear. In the present study, we proved that CR-1 was expressed in PCa and its function was in the progression of PCa. Compared with benign prostatic hyperplasia (BPH) tissues, we confirmed that PCa tissues had high expression of CR-1 by immunohistochemistry and statistical data showed that CR-1 promoted properties of EMT in PCa tissues, including the downregulation of the cell adhesion molecules β-catenin (membrane) and E-cadherin while upregulating transcription factors β-catenin. Overexpression of CR-1 had close relationship with PSA, Gleason, clinical staging and lymph node metastasis in PCa patients. Then, we found that PC-3 cells transfected with CR-1-shRNA inhibited EMT using RT-PCR, RT-qPCR, western blotting and immunofluorescence. Also, we evaluated cell invasive ability in vitro by transwell and wound-healing assay. Our data showed that transfected CR-1-shRNA altered EMT including β-catenin, E-cadherin, c-myc, GSK-3, p-GSK and Wnt/β-catenin pathway in PC-3. It also suppressed PC-3 cell migration. Additionally, our results displayed that Licl had antitumor activity against PC-3 through the inhibition of Wnt/β-catenin pathway. Inhibition of cell viability was dose-time dependent. The present study proved that CR-1 regulates EMT of PCa by Wnt/β-catenin pathway. Hence, CR-1 may provide a new biological marker, and possibly contributes to clinical treatment against PCa.
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1 

Siegel RL, Miller KD and Jemal A: Cancer statistics, 2016. CA Cancer J Clin. 66:7–30. 2016. View Article : Google Scholar : PubMed/NCBI

2 

Center MM, Jemal A, Lortet-Tieulent J, Ward E, Ferlay J, Brawley O and Bray F: International variation in prostate cancer incidence and mortality rates. Eur Urol. 61:1079–1092. 2012. View Article : Google Scholar : PubMed/NCBI

3 

Attard G, Parker C, Eeles RA, Schröder F, Tomlins SA, Tannock I, Drake CG and de Bono JS: Prostate cancer. Lancet. 387:70–82. 2016. View Article : Google Scholar : PubMed/NCBI

4 

Saloman DS, Bianco C, Ebert AD, Khan NI, De Santis M, Normanno N, Wechselberger C, Seno M, Williams K, Sanicola M, et al: The EGF-CFC family: Novel epidermal growth factor-related proteins in development and cancer. Endocr Relat Cancer. 7:199–226. 2000. View Article : Google Scholar : PubMed/NCBI

5 

Ciccodicola A, Dono R, Obici S, Simeone A, Zollo M and Persico MG: Molecular characterization of a gene of the ‘EGF family’ expressed in undifferentiated human NTERA2 teratocarcinoma cells. EMBO J. 8:1987–1991. 1989.PubMed/NCBI

6 

Bianco C, Rangel MC, Castro NP, Nagaoka T, Rollman K, Gonzales M and Salomon DS: Role of Cripto-1 in stem cell maintenance and malignant progression. Am J Pathol. 177:532–540. 2010. View Article : Google Scholar : PubMed/NCBI

7 

Bianco C, Strizzi L, Normanno N, Khan N and Salomon DS: Cripto-1: An oncofetal gene with many faces. Curr Top Dev Biol. 67:85–133. 2005. View Article : Google Scholar : PubMed/NCBI

8 

De Castro NP, Rangel MC, Nagaoka T, Salomon DS and Bianco C: Cripto-1: An embryonic gene that promotes tumorigenesis. Future Oncol. 6:1127–1142. 2010. View Article : Google Scholar : PubMed/NCBI

9 

Strizzi L, Bianco C, Normanno N, Seno M, Wechselberger C, Wallace-Jones B, Khan NI, Hirota M, Sun Y, Sanicola M, et al: Epithelial mesenchymal transition is a characteristic of hyperplasias and tumors in mammary gland from MMTV-Cripto-1 transgenic mice. J Cell Physiol. 201:266–276. 2004. View Article : Google Scholar : PubMed/NCBI

10 

Wechselberger C, Ebert AD, Bianco C, Khan NI, Sun Y, Wallace-Jones B, Montesano R and Salomon DS: Cripto-1 enhances migration and branching morphogenesis of mouse mammary epithelial cells. Exp Cell Res. 266:95–105. 2001. View Article : Google Scholar : PubMed/NCBI

11 

Wechselberger C, Strizzi L, Kenney N, Hirota M, Sun Y, Ebert A, Orozco O, Bianco C, Khan NI, Wallace-Jones B, et al: Human Cripto-1 overexpression in the mouse mammary gland results in the development of hyperplasia and adenocarcinoma. Oncogene. 24:4094–4105. 2005. View Article : Google Scholar : PubMed/NCBI

12 

Strizzi L, Bianco C, Normanno N and Salomon D: Cripto-1: A multifunctional modulator during embryogenesis and oncogenesis. Oncogene. 24:5731–5741. 2005. View Article : Google Scholar : PubMed/NCBI

13 

Rangel MC, Karasawa H, Castro NP, Nagaoka T, Salomon DS and Bianco C: Role of Cripto-1 during epithelial-to-mesenchymal transition in development and cancer. Am J Pathol. 180:2188–2200. 2012. View Article : Google Scholar : PubMed/NCBI

14 

Acloque H, Adams MS, Fishwick K, Bronner-Fraser M and Nieto MA: Epithelial-mesenchymal transitions: The importance of changing cell state in development and disease. J Clin Invest. 119:1438–1449. 2009. View Article : Google Scholar : PubMed/NCBI

15 

Prasad CP, Rath G, Mathur S, Bhatnagar D, Parshad R and Ralhan R: Expression analysis of E-cadherin, Slug and GSK3beta in invasive ductal carcinoma of breast. BMC Cancer. 9:3252009. View Article : Google Scholar : PubMed/NCBI

16 

Li Y, Che Q, Bian Y, Zhou Q, Jiang F, Tong H, Ke J, Wang K and Wan XP: Autocrine motility factor promotes epithelial-mesenchymal transition in endometrial cancer via MAPK signaling pathway. Int J Oncol. 47:1017–1024. 2015.PubMed/NCBI

17 

Adamson ED, Minchiotti G and Salomon DS: Cripto: A tumor growth factor and more. J Cell Physiol. 190:267–278. 2002. View Article : Google Scholar : PubMed/NCBI

18 

Bianco C, Normanno N, De Luca A, Maiello MR, Wechselberger C, Sun Y, Khan N, Adkins H, Sanicola M, Vonderhaar B, et al: Detection and localization of Cripto-1 binding in mouse mammary epithelial cells and in the mouse mammary gland using an immunoglobulin-cripto-1 fusion protein. J Cell Physiol. 190:74–82. 2002. View Article : Google Scholar : PubMed/NCBI

19 

Wei B, Jin W, Ruan J, Xu Z, Zhou Y, Liang J, Cheng H, Jin K, Huang X, Lu P, et al: Cripto-1 expression and its prognostic value in human bladder cancer patients. Tumour Biol. 36:1105–1113. 2015. View Article : Google Scholar : PubMed/NCBI

20 

Wang JH, Wei W, Xu J, Guo ZX, Xiao CZ, Zhang YF, Jian PE, Wu XL, Shi M and Guo RP: Elevated expression of Cripto-1 correlates with poor prognosis in hepatocellular carcinoma. Oncotarget. 6:35116–35128. 2015.PubMed/NCBI

21 

Nagaoka T, Karasawa H, Castro NP, Rangel MC, Salomon DS and Bianco C: An evolving web of signaling networks regulated by Cripto-1. Growth Factors. 30:13–21. 2012. View Article : Google Scholar : PubMed/NCBI

22 

Bianco C, Castro NP, Baraty C, Rollman K, Held N, Rangel MC, Karasawa H, Gonzales M, Strizzi L and Salomon DS: Regulation of human Cripto-1 expression by nuclear receptors and DNA promoter methylation in human embryonal and breast cancer cells. J Cell Physiol. 228:1174–1188. 2013. View Article : Google Scholar : PubMed/NCBI

23 

Shukla A, Ho Y, Liu X, Ryscavage A and Glick AB: Cripto-1 alters keratinocyte differentiation via blockade of transforming growth factor-beta1 signaling: Role in skin carcinogenesis. Mol Cancer Res. 6:509–516. 2008. View Article : Google Scholar : PubMed/NCBI

24 

Xu CH, Wang Y, Qian LH, Yu LK, Zhang XW and Wang QB: Serum Cripto-1 is a novel biomarker for non-small cell lung cancer diagnosis and prognosis. Clin Respir J. Nov 25–2015.(Epub ahead of print) doi: 10.1111/crj.12414. View Article : Google Scholar

25 

Gong YP, Yarrow PM, Carmalt HL, Kwun SY, Kennedy CW, Lin BP, Xing PX and Gillett DJ: Overexpression of Cripto and its prognostic significance in breast cancer: A study with long-term survival. Eur J Surg Oncol. 33:438–443. 2007. View Article : Google Scholar : PubMed/NCBI

26 

Yang J and Weinberg RA: Epithelial-mesenchymal transition: At the crossroads of development and tumor metastasis. Dev Cell. 14:818–829. 2008. View Article : Google Scholar : PubMed/NCBI

27 

Klauzinska M, Castro NP, Rangel MC, Spike BT, Gray PC, Bertolette D, Cuttitta F and Salomon D: The multifaceted role of the embryonic gene Cripto-1 in cancer, stem cells and epithelial-mesenchymal transition. Semin Cancer Biol. 29:51–58. 2014. View Article : Google Scholar : PubMed/NCBI

28 

Zhong XY, Zhang LH, Jia SQ, Shi T, Niu ZJ, Du H, Zhang GG, Hu Y, Lu AP, Li JY, et al: Positive association of up-regulated Cripto-1 and down-regulated E-cadherin with tumour progression and poor prognosis in gastric cancer. Histopathology. 52:560–568. 2008. View Article : Google Scholar : PubMed/NCBI

29 

Strizzi L, Bianco C, Raafat A, Abdallah W, Chang C, Raafat D, Hirota M, Hamada S, Sun Y, Normanno N, et al: Netrin-1 regulates invasion and migration of mouse mammary epithelial cells overexpressing Cripto-1 in vitro and in vivo. J Cell Sci. 118:4633–4643. 2005. View Article : Google Scholar : PubMed/NCBI

30 

Hou CL, Zhang ZH, Huang DL and Sun AJ: LiCl suppresses tumor growth and inhibits DNA replication in prostate cancer. Zhonghua Bing Li Xue Za Zhi. 41:475–478. 2012.(In Chinese). PubMed/NCBI

31 

Zhang F, Phiel CJ, Spece L, Gurvich N and Klein PS: Inhibitory phosphorylation of glycogen synthase kinase-3 (GSK-3) in response to lithium. Evidence for autoregulation of GSK-3. J Biol Chem. 278:33067–33077. 2003. View Article : Google Scholar : PubMed/NCBI

32 

Huang C, Chen W, Wang X, Zhao J, Li Q and Fu Z: Cripto-1 promotes the epithelial-mesenchymal transition in esophageal squamous cell carcinoma cells. Evid Based Complement Alternat Med. 2015:4212852015. View Article : Google Scholar : PubMed/NCBI

33 

Wu Z, Li G, Wu L, Weng D, Li X and Yao K: Cripto-1 overexpression is involved in the tumorigenesis of nasopharyngeal carcinoma. BMC Cancer. 9:3152009. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Liu Y, Qin Z, Yang K, Liu R and Xu Y: Cripto-1 promotes epithelial-mesenchymal transition in prostate cancer via Wnt/β-catenin signaling Corrigendum in /10.3892/or.2024.8734. Oncol Rep 37: 1521-1528, 2017.
APA
Liu, Y., Qin, Z., Yang, K., Liu, R., & Xu, Y. (2017). Cripto-1 promotes epithelial-mesenchymal transition in prostate cancer via Wnt/β-catenin signaling Corrigendum in /10.3892/or.2024.8734. Oncology Reports, 37, 1521-1528. https://doi.org/10.3892/or.2017.5378
MLA
Liu, Y., Qin, Z., Yang, K., Liu, R., Xu, Y."Cripto-1 promotes epithelial-mesenchymal transition in prostate cancer via Wnt/β-catenin signaling Corrigendum in /10.3892/or.2024.8734". Oncology Reports 37.3 (2017): 1521-1528.
Chicago
Liu, Y., Qin, Z., Yang, K., Liu, R., Xu, Y."Cripto-1 promotes epithelial-mesenchymal transition in prostate cancer via Wnt/β-catenin signaling Corrigendum in /10.3892/or.2024.8734". Oncology Reports 37, no. 3 (2017): 1521-1528. https://doi.org/10.3892/or.2017.5378
Copy and paste a formatted citation
x
Spandidos Publications style
Liu Y, Qin Z, Yang K, Liu R and Xu Y: Cripto-1 promotes epithelial-mesenchymal transition in prostate cancer via Wnt/β-catenin signaling Corrigendum in /10.3892/or.2024.8734. Oncol Rep 37: 1521-1528, 2017.
APA
Liu, Y., Qin, Z., Yang, K., Liu, R., & Xu, Y. (2017). Cripto-1 promotes epithelial-mesenchymal transition in prostate cancer via Wnt/β-catenin signaling Corrigendum in /10.3892/or.2024.8734. Oncology Reports, 37, 1521-1528. https://doi.org/10.3892/or.2017.5378
MLA
Liu, Y., Qin, Z., Yang, K., Liu, R., Xu, Y."Cripto-1 promotes epithelial-mesenchymal transition in prostate cancer via Wnt/β-catenin signaling Corrigendum in /10.3892/or.2024.8734". Oncology Reports 37.3 (2017): 1521-1528.
Chicago
Liu, Y., Qin, Z., Yang, K., Liu, R., Xu, Y."Cripto-1 promotes epithelial-mesenchymal transition in prostate cancer via Wnt/β-catenin signaling Corrigendum in /10.3892/or.2024.8734". Oncology Reports 37, no. 3 (2017): 1521-1528. https://doi.org/10.3892/or.2017.5378
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