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Article

MicroRNA-9 limits hepatic fibrosis by suppressing the activation and proliferation of hepatic stellate cells by directly targeting MRP1/ABCC1

Retraction in: /10.3892/or.2023.8590
  • Authors:
    • Jie Sun
    • Huanying Zhang
    • Liying Li
    • Lianfeng Yu
    • Lifang Fu
  • View Affiliations / Copyright

    Affiliations: Department of Traditional Chinese Medicine, Shandong Medical College, Linyi, Shandong 276000, P.R. China, Department of Respiration, Affiliated Hospital of Shandong Medical College, Linyi, Shandong 276000, P.R. China, Department of Rehabilitation Medicine, Shandong Medical College, Linyi, Shandong 276000, P.R. China, Department of Anatomy, Shandong Medical College, Linyi, Shandong 276000, P.R. China, Department of Respiration, Chinese Medicine Hospital of Linyi City, Linyi, Shandong 276000, P.R. China
  • Pages: 1698-1706
    |
    Published online on: January 17, 2017
       https://doi.org/10.3892/or.2017.5382
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Abstract

Liver fibrosis is a chronic liver disease characterized by the proliferation and activation of hepatic stellate cells (HSCs) and excessive deposition of extracellular matrix (ECM). Research suggests that microRNAs (miRNAs) are a new type of regulator of liver fibrosis. In the present study, we investigated the role of microRNA-9 (miR-9) in the process of liver fibrosis, as well as the underlying mechanism of action. Downregulated levels of miR-9 were found in fibrotic liver tissues and activated HSCs as detected by qRT-PCR; whereas, expression of multidrug resistance‑associated protein 1 (MRP1/ABCC1) was upregulated in the fibrotic liver tissues and activated HSCs. CCK-8 and BrdU assays revealed that miR-9 reduced the proliferative ability of the HSCs. In addition, expression levels of ECM-related genes (α-SMA, Col-1 and Timp-1), which are markers of HSC activation, were downregulated by miR-9. Conversely, an miR-9 inhibitor promoted cell proliferation and HSC activation. In addition, a luciferase reporter assay indicated that miR-9 targets the 3'-untranslated region (3'-UTR) of MRP1 and causes a significant decrease in MRP1. miR-9 inhibited the activation of the Hedgehog (Hh) pathway and the expression of MRP1, while this suppression was rescued by the overexpression of MRP1. Finally, a CCl4-induced mouse model of liver fibrosis was used to investigate the effects of miR-9 on liver fibrosis in vivo. The results showed that miR-9 abrogated hepatic fibrosis by suppressing the expression of MRP1 in CCl4-induced liver fibrotic mice. In conclusion, the present study demonstrated that miR-9 suppresses the proliferation and activation of HSCs through the Hh pathway by targeting MRP1, which suggests that miR-9 has therapeutic potential for liver fibrosis.
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Copy and paste a formatted citation
Spandidos Publications style
Sun J, Zhang H, Li L, Yu L and Fu L: MicroRNA-9 limits hepatic fibrosis by suppressing the activation and proliferation of hepatic stellate cells by directly targeting MRP1/ABCC1 Retraction in /10.3892/or.2023.8590. Oncol Rep 37: 1698-1706, 2017.
APA
Sun, J., Zhang, H., Li, L., Yu, L., & Fu, L. (2017). MicroRNA-9 limits hepatic fibrosis by suppressing the activation and proliferation of hepatic stellate cells by directly targeting MRP1/ABCC1 Retraction in /10.3892/or.2023.8590. Oncology Reports, 37, 1698-1706. https://doi.org/10.3892/or.2017.5382
MLA
Sun, J., Zhang, H., Li, L., Yu, L., Fu, L."MicroRNA-9 limits hepatic fibrosis by suppressing the activation and proliferation of hepatic stellate cells by directly targeting MRP1/ABCC1 Retraction in /10.3892/or.2023.8590". Oncology Reports 37.3 (2017): 1698-1706.
Chicago
Sun, J., Zhang, H., Li, L., Yu, L., Fu, L."MicroRNA-9 limits hepatic fibrosis by suppressing the activation and proliferation of hepatic stellate cells by directly targeting MRP1/ABCC1 Retraction in /10.3892/or.2023.8590". Oncology Reports 37, no. 3 (2017): 1698-1706. https://doi.org/10.3892/or.2017.5382
Copy and paste a formatted citation
x
Spandidos Publications style
Sun J, Zhang H, Li L, Yu L and Fu L: MicroRNA-9 limits hepatic fibrosis by suppressing the activation and proliferation of hepatic stellate cells by directly targeting MRP1/ABCC1 Retraction in /10.3892/or.2023.8590. Oncol Rep 37: 1698-1706, 2017.
APA
Sun, J., Zhang, H., Li, L., Yu, L., & Fu, L. (2017). MicroRNA-9 limits hepatic fibrosis by suppressing the activation and proliferation of hepatic stellate cells by directly targeting MRP1/ABCC1 Retraction in /10.3892/or.2023.8590. Oncology Reports, 37, 1698-1706. https://doi.org/10.3892/or.2017.5382
MLA
Sun, J., Zhang, H., Li, L., Yu, L., Fu, L."MicroRNA-9 limits hepatic fibrosis by suppressing the activation and proliferation of hepatic stellate cells by directly targeting MRP1/ABCC1 Retraction in /10.3892/or.2023.8590". Oncology Reports 37.3 (2017): 1698-1706.
Chicago
Sun, J., Zhang, H., Li, L., Yu, L., Fu, L."MicroRNA-9 limits hepatic fibrosis by suppressing the activation and proliferation of hepatic stellate cells by directly targeting MRP1/ABCC1 Retraction in /10.3892/or.2023.8590". Oncology Reports 37, no. 3 (2017): 1698-1706. https://doi.org/10.3892/or.2017.5382
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