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Article

Circular RNA and gene expression profiles in gastric cancer based on microarray chip technology

  • Authors:
    • Weiguo Sui
    • Zhoufang Shi
    • Wen Xue
    • Minglin Ou
    • Ying Zhu
    • Jiejing Chen
    • Hua Lin
    • Fuhua Liu
    • Yong Dai
  • View Affiliations / Copyright

    Affiliations: Nephrology Department of Guilin No. 181 Hospital, Guangxi Key Laboratory of Metabolic Diseases Research, Guilin, Guangxi 541004, P.R. China, College of Life Science of Guangxi Normal University, Guilin, Guangxi 541004, P.R. China, Clinical Medical Research Center, The Second Clinical Medical, College of Jinan University, Shenzhen People's Hospital, Shenzhen, Guangdong 518020, P.R. China
  • Pages: 1804-1814
    |
    Published online on: February 1, 2017
       https://doi.org/10.3892/or.2017.5415
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Abstract

The aim of the present study was to screen gastric cancer (GC) tissue and adjacent tissue for differences in mRNA and circular (circRNA) expression, to analyze the differences in circRNA and mRNA expression, and to investigate the circRNA expression in gastric carcinoma and its mechanism. circRNA and mRNA differential expression profiles generated using Agilent microarray technology were analyzed in the GC tissues and adjacent tissues. qRT-PCR was used to verify the differential expression of circRNAs and mRNAs according to the interactions between circRNAs and miRNAs as well as the possible existence of miRNA and mRNA interactions. We found that: i) the circRNA expression profile revealed 1,285 significant differences in circRNA expression, with circRNA expression downregulated in 594 samples and upregulated in 691 samples via interactions with miRNAs. The qRT-PCR validation experiments showed that hsa_circRNA_400071, hsa_circRNA_000543 and hsa_circRNA_001959 expression was consistent with the microarray analysis results. ii) 29,112 genes were found in the GC tissues and adjacent tissues, including 5,460 differentially expressed genes. Among them, 2,390 differentially expressed genes were upregulated and 3,070 genes were downregulated. Gene Ontology (GO) analysis of the differentially expressed genes revealed these genes involved in biological process classification, cellular component classification and molecular function classification. Pathway analysis of the differentially expressed genes identified 83 significantly enriched genes, including 28 upregulated genes and 55 downregulated genes. iii) 69 differentially expressed circRNAs were found that might adsorb specific miRNAs to regulate the expression of their target gene mRNAs. The conclusions are: i) differentially expressed circRNAs had corresponding miRNA binding sites. These circRNAs regulated the expression of target genes through interactions with miRNAs and might become new molecular biomarkers for GC in the future. ii) Differentially expressed genes may be involved in the occurrence of GC via a variety of mechanisms. iii) CD44, CXXC5, MYH9, MALAT1 and other genes may have important implications for the occurrence and development of GC through the regulation, interaction, and mutual influence of circRNA-miRNA-mRNA via different mechanisms.
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View References

1 

Jemal A, Bray F, Center MM, Ferlay J, Ward E and Forman D: Global cancer statistics. CA Cancer J Clin. 61:69–90. 2011. View Article : Google Scholar : PubMed/NCBI

2 

Park DJ, Thomas NJ, Yoon C and Yoon SS: Vascular endothelial growth factor a inhibition in gastric cancer. Gastric Cancer. 18:33–42. 2015. View Article : Google Scholar : PubMed/NCBI

3 

Karimi P, Islami F, Anandasabapathy S, Freedman ND and Kamangar F: Gastric cancer: Descriptive epidemiology, risk factors, screening, and prevention. Cancer Epidemiol Biomarkers Prev. 23:700–713. 2014. View Article : Google Scholar : PubMed/NCBI

4 

Degiuli M and Calvo F: Survival of early gastric cancer in a specialized European center. Which lymphadenectomy is necessary? World J Surg. 30:2193–2203. 2006. View Article : Google Scholar : PubMed/NCBI

5 

Schwarz RE and Smith DD: Clinical impact of lymphadenectomy extent in resectable gastric cancer of advanced stage. Ann Surg Oncol. 14:317–328. 2007. View Article : Google Scholar : PubMed/NCBI

6 

Wilusz JE and Sharp PA: Molecular biology. A circuitous route to noncoding RNA. Science. 340:440–441. 2013. View Article : Google Scholar : PubMed/NCBI

7 

Hansen TB, Jensen TI, Clausen BH, Bramsen JB, Finsen B, Damgaard CK and Kjems J: Natural RNA circles function as efficient microRNA sponges. Nature. 495:384–388. 2013. View Article : Google Scholar : PubMed/NCBI

8 

Maass PG, Luft FC and Bähring S: Long non-coding RNA in health and disease. J Mol Med (Berl). 92:337–346. 2014. View Article : Google Scholar : PubMed/NCBI

9 

Valdmanis PN and Kay MA: The expanding repertoire of circular RNAs. Mol Ther. 21:1112–1114. 2013. View Article : Google Scholar : PubMed/NCBI

10 

Burd CE, Jeck WR, Liu Y, Sanoff HK, Wang Z and Sharpless NE: Expression of linear and novel circular forms of an INK4/ARF-associated non-coding RNA correlates with atherosclerosis risk. PLoS Genet. 6:e10012332010. View Article : Google Scholar : PubMed/NCBI

11 

Jeck WR, Sorrentino JA, Wang K, Slevin MK, Burd CE, Liu J, Marzluff WF and Sharpless NE: Circular RNAs are abundant, conserved, and associated with ALU repeats. RNA. 19:141–157. 2013. View Article : Google Scholar : PubMed/NCBI

12 

Li Z, Huang C, Bao C, Chen L, Lin M, Wang X, Zhong G, Yu B, Hu W, Dai L, et al: Exon-intron circular RNAs regulate transcription in the nucleus. Nat Struct Mol Biol. 22:256–264. 2015. View Article : Google Scholar : PubMed/NCBI

13 

Li F, Zhang L, Li W, Deng J, Zheng J, An M, Lu J and Zhou Y: Circular RNA ITCH has inhibitory effect on ESCC by suppressing the Wnt/β-catenin pathway. Oncotarget. 6:6001–6013. 2015. View Article : Google Scholar : PubMed/NCBI

14 

Dai Y, Sui W, Lan H, Yan Q, Huang H and Huang Y: Comprehensive analysis of microRNA expression patterns in renal biopsies of lupus nephritis patients. Rheumatol Int. 29:749–754. 2009. View Article : Google Scholar : PubMed/NCBI

15 

Memczak S, Jens M, Elefsinioti A, Torti F, Krueger J, Rybak A, Maier L, Mackowiak SD, Gregersen LH, Munschauer M, et al: Circular RNAs are a large class of animal RNAs with regulatory potency. Nature. 495:333–338. 2013. View Article : Google Scholar : PubMed/NCBI

16 

Salzman J, Gawad C, Wang PL, Lacayo N and Brown PO: Circular RNAs are the predominant transcript isoform from hundreds of human genes in diverse cell types. PLoS One. 7:e307332012. View Article : Google Scholar : PubMed/NCBI

17 

Ghosal S, Das S, Sen R, Basak P and Chakrabarti J: Circ2Traits: A comprehensive database for circular RNA potentially associated with disease and traits. Front Genet. 4:2832013. View Article : Google Scholar : PubMed/NCBI

18 

Doberstein K, Bretz NP, Schirmer U, Fiegl H, Blaheta R, Breunig C, Müller-Holzner E, Reimer D, Zeimet AG and Altevogt P: miR-21-3p is a positive regulator of L1CAM in several human carcinomas. Cancer Lett. 354:455–466. 2014. View Article : Google Scholar : PubMed/NCBI

19 

Fukuhara S, Sakurai A, Sano H, Yamagishi A, Somekawa S, Takakura N, Saito Y, Kangawa K and Mochizuki N: Cyclic AMP potentiates vascular endothelial cadherin-mediated cell-cell contact to enhance endothelial barrier function through an Epac-Rap1 signaling pathway. Mol Cell Biol. 25:136–146. 2005. View Article : Google Scholar : PubMed/NCBI

20 

Arai K, Sakamoto R, Kubota D and Kondo T: Proteomic approach toward molecular backgrounds of drug resistance of osteosarcoma cells in spheroid culture system. Proteomics. 13:2351–2360. 2013. View Article : Google Scholar : PubMed/NCBI

21 

Cho HJ, Baek KE, Kim IK, Park SM, Choi YL, Nam IK, Park SH, Im MJ, Yoo JM, Ryu KJ, et al: Proteomics-based strategy to delineate the molecular mechanisms of RhoGDI2-induced metastasis and drug resistance in gastric cancer. J Proteome Res. 11:2355–2364. 2012. View Article : Google Scholar : PubMed/NCBI

22 

PLoS One Editors, . Retraction: GREB1 functions as a growth promoter and is modulated by IL6/STAT3 in breast cancer. PLoS One. 9:e1022872014. View Article : Google Scholar : PubMed/NCBI

23 

Akiyama H, Watanabe T, Wakabayashi K, Nakade S, Yasui S, Sakata K, Chiba R, Spiegelhalter F, Hino A and Maitani T: Quantitative detection system for maize sample containing combined-trait genetically modified maize. Anal Chem. 77:7421–7428. 2005. View Article : Google Scholar : PubMed/NCBI

24 

Feigin VL and Findlay M: Advances in subarachnoid hemorrhage. Stroke. 37:305–308. 2006. View Article : Google Scholar : PubMed/NCBI

25 

van der Voet M, Olson JM, Kuivaniemi H, Dudek DM, Skunca M, Ronkainen A, Niemelä M, Jääskeläinen J, Hernesniemi J, Helin K, et al: Intracranial aneurysms in Finnish families: Confirmation of linkage and refinement of the interval to chromosome 19q13.3. Am J Hum Genet. 74:564–571. 2004. View Article : Google Scholar : PubMed/NCBI

26 

Juvela S: Natural history of unruptured intracranial aneurysms: Risks for aneurysm formation, growth, and rupture. Acta Neurochir Suppl. 82:27–30. 2002.PubMed/NCBI

27 

Liang S, He L, Zhao X, Miao Y, Gu Y, Guo C, Xue Z, Dou W, Hu F, Wu K, et al: MicroRNA let-7f inhibits tumor invasion and metastasis by targeting MYH9 in human gastric cancer. PLoS One. 6:e184092011. View Article : Google Scholar : PubMed/NCBI

28 

Naor D, Nedvetzki S, Golan I, Melnik L and Faitelson Y: CD44 in cancer. Crit Rev Clin Lab Sci. 39:527–579. 2002. View Article : Google Scholar : PubMed/NCBI

29 

Gutschner T, Hämmerle M and Diederichs S: MALAT1 - a paradigm for long noncoding RNA function in cancer. J Mol Med (Berl). 91:791–801. 2013. View Article : Google Scholar : PubMed/NCBI

30 

Wang JH, Ren Y, Zhang R, Han Y, Sheng YH, Hou WJ and Ao HF: Effects of CXXC finger protein 5 up-regulated expression in epithelial ovarian cancer. China Oncol. 25:260–268. 2015.(In Chinese). http://www.china-oncology.com/EN/Y2015/V25/I4/260

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Copy and paste a formatted citation
Spandidos Publications style
Sui W, Shi Z, Xue W, Ou M, Zhu Y, Chen J, Lin H, Liu F and Dai Y: Circular RNA and gene expression profiles in gastric cancer based on microarray chip technology. Oncol Rep 37: 1804-1814, 2017.
APA
Sui, W., Shi, Z., Xue, W., Ou, M., Zhu, Y., Chen, J. ... Dai, Y. (2017). Circular RNA and gene expression profiles in gastric cancer based on microarray chip technology. Oncology Reports, 37, 1804-1814. https://doi.org/10.3892/or.2017.5415
MLA
Sui, W., Shi, Z., Xue, W., Ou, M., Zhu, Y., Chen, J., Lin, H., Liu, F., Dai, Y."Circular RNA and gene expression profiles in gastric cancer based on microarray chip technology". Oncology Reports 37.3 (2017): 1804-1814.
Chicago
Sui, W., Shi, Z., Xue, W., Ou, M., Zhu, Y., Chen, J., Lin, H., Liu, F., Dai, Y."Circular RNA and gene expression profiles in gastric cancer based on microarray chip technology". Oncology Reports 37, no. 3 (2017): 1804-1814. https://doi.org/10.3892/or.2017.5415
Copy and paste a formatted citation
x
Spandidos Publications style
Sui W, Shi Z, Xue W, Ou M, Zhu Y, Chen J, Lin H, Liu F and Dai Y: Circular RNA and gene expression profiles in gastric cancer based on microarray chip technology. Oncol Rep 37: 1804-1814, 2017.
APA
Sui, W., Shi, Z., Xue, W., Ou, M., Zhu, Y., Chen, J. ... Dai, Y. (2017). Circular RNA and gene expression profiles in gastric cancer based on microarray chip technology. Oncology Reports, 37, 1804-1814. https://doi.org/10.3892/or.2017.5415
MLA
Sui, W., Shi, Z., Xue, W., Ou, M., Zhu, Y., Chen, J., Lin, H., Liu, F., Dai, Y."Circular RNA and gene expression profiles in gastric cancer based on microarray chip technology". Oncology Reports 37.3 (2017): 1804-1814.
Chicago
Sui, W., Shi, Z., Xue, W., Ou, M., Zhu, Y., Chen, J., Lin, H., Liu, F., Dai, Y."Circular RNA and gene expression profiles in gastric cancer based on microarray chip technology". Oncology Reports 37, no. 3 (2017): 1804-1814. https://doi.org/10.3892/or.2017.5415
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