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Article

miR-181a-5p, an inducer of Wnt-signaling, facilitates cell proliferation in acute lymphoblastic leukemia

  • Authors:
    • Xiaoming Lyu
    • Jinbang Li
    • Xi Yun
    • Rui Huang
    • Xubin Deng
    • Yingping Wang
    • Youming Chen
    • Gang Xiao
  • View Affiliations / Copyright

    Affiliations: Department of Laboratory Medicine, The Third Affiliated Hospital of Southern Medical University, Guangzhou, Guangdong, P.R. China, Department of Pathology, Qingyuan People's Hospital, Jinan University, Qingyuan, Guangdong, P.R. China, Department of Clinical Laboratory, The Third Affiliated Hospital of Sun Yet-sen University, Guangzhou, Guangdong, P.R. China, Department of Hematology, Zhujiang Hospital, Southern Medical University, Guangzhou, Guangdong, P.R. China, Department of Medical Oncology, Affiliated Cancer Hospital of Guangzhou Medical University, Guangzhou, Guangdong, P.R. China, Department of Biochemistry and Molecular Cell Biology, Shanghai Key Laboratory for Tumor Microenvironment and Inflammation, Institute of Medical Sciences, Shanghai Jiao Tong University School of Medicine, Shanghai, P.R. China
  • Pages: 1469-1476
    |
    Published online on: February 6, 2017
       https://doi.org/10.3892/or.2017.5425
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Abstract

Uncontrolled Wnt signaling causes leukemia. Inactivation of Wnt antagonists could play an important role in leukemia progression by activating the Wnt/β-catenin pathway. Wnt inhibitory factor-1 (WIF1) is one of the important Wnt antagonists. Few miRNAs have been reported to directly target this gene in hematopoiesis. Here, we observed that miR-181a-5p expression was markedly overexpressed in several leukemia cell lines and acute lymphoblastic leukemia (ALL) samples compared with that noted in normal peripheral blood mononuclear cells. MTT assays, soft agar colony formation assays and flow cytometry analysis collectively showed that ectopic expression of miR-181a-5p induced ALL cell growth and proliferation. Furthermore, a mechanistic study disclosed that miR-181a-5p directly downregulated WIF1 expression by binding to its 3'-UTR, and further activated Wnt/β‑catenin signaling. These findings provide a novel mechanistic insight into the role of miR-181a-5p in ALL cell growth and proliferation and implicate miR-181a-5p as an attractive candidate for ALL therapy.
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Copy and paste a formatted citation
Spandidos Publications style
Lyu X, Li J, Yun X, Huang R, Deng X, Wang Y, Chen Y and Xiao G: miR-181a-5p, an inducer of Wnt-signaling, facilitates cell proliferation in acute lymphoblastic leukemia. Oncol Rep 37: 1469-1476, 2017.
APA
Lyu, X., Li, J., Yun, X., Huang, R., Deng, X., Wang, Y. ... Xiao, G. (2017). miR-181a-5p, an inducer of Wnt-signaling, facilitates cell proliferation in acute lymphoblastic leukemia. Oncology Reports, 37, 1469-1476. https://doi.org/10.3892/or.2017.5425
MLA
Lyu, X., Li, J., Yun, X., Huang, R., Deng, X., Wang, Y., Chen, Y., Xiao, G."miR-181a-5p, an inducer of Wnt-signaling, facilitates cell proliferation in acute lymphoblastic leukemia". Oncology Reports 37.3 (2017): 1469-1476.
Chicago
Lyu, X., Li, J., Yun, X., Huang, R., Deng, X., Wang, Y., Chen, Y., Xiao, G."miR-181a-5p, an inducer of Wnt-signaling, facilitates cell proliferation in acute lymphoblastic leukemia". Oncology Reports 37, no. 3 (2017): 1469-1476. https://doi.org/10.3892/or.2017.5425
Copy and paste a formatted citation
x
Spandidos Publications style
Lyu X, Li J, Yun X, Huang R, Deng X, Wang Y, Chen Y and Xiao G: miR-181a-5p, an inducer of Wnt-signaling, facilitates cell proliferation in acute lymphoblastic leukemia. Oncol Rep 37: 1469-1476, 2017.
APA
Lyu, X., Li, J., Yun, X., Huang, R., Deng, X., Wang, Y. ... Xiao, G. (2017). miR-181a-5p, an inducer of Wnt-signaling, facilitates cell proliferation in acute lymphoblastic leukemia. Oncology Reports, 37, 1469-1476. https://doi.org/10.3892/or.2017.5425
MLA
Lyu, X., Li, J., Yun, X., Huang, R., Deng, X., Wang, Y., Chen, Y., Xiao, G."miR-181a-5p, an inducer of Wnt-signaling, facilitates cell proliferation in acute lymphoblastic leukemia". Oncology Reports 37.3 (2017): 1469-1476.
Chicago
Lyu, X., Li, J., Yun, X., Huang, R., Deng, X., Wang, Y., Chen, Y., Xiao, G."miR-181a-5p, an inducer of Wnt-signaling, facilitates cell proliferation in acute lymphoblastic leukemia". Oncology Reports 37, no. 3 (2017): 1469-1476. https://doi.org/10.3892/or.2017.5425
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