Enhanced expression of PKM2 associates with the biological properties of cancer stem cells from A549 human lung cancer cells

  • Authors:
    • Chang-Ying Guo
    • Chen Yan
    • Lan Luo
    • Shinji Goto
    • Yoshishige Urata
    • Jian-Jun Xu
    • Xiao-Ming Wen
    • Yu-Kang Kuang
    • Fang-Fang Tou
    • Tao-Sheng Li
  • View Affiliations

  • Published online on: February 10, 2017     https://doi.org/10.3892/or.2017.5438
  • Pages: 2161-2166
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Abstract

Cancer cells express the M2 isoform of glycolytic enzyme pyruvate kinase (PKM2) for favoring the survival under a hypoxic condition. Considering the relative low oxygen microenvironment in stem cell niche, we hypothesized that an enhanced PKM2 expression associates with the biological properties of cancer stem cells. We used A549 human lung cancer cell line and surgical resected lung cancer tissue samples from patients for experiments. We confirmed the co-localization of PKM2 and CD44, a popular marker for cancer stem cells in lung cancer tissue samples from patients. The expression of PKM2 was clearly observed in approximately 80% of the A549 human lung cancer cells. Remarkably, enhanced expression of PKM2 was specially observed in these cells that also positively expressed CD44. Downregulation of PKM2 in CD44+ cancer stem cells by siRNA significantly impaired the potency for spheroid formation, decreased the cell survival under fetal bovine serum deprivation and hypoxic conditions, but increased their sensitivity to anti-cancer drug of cisplatin and γ-ray. The enhanced expression of PKM2 seems to associate with the biological properties of cancer stem cells from A549 human lung cancer cells. Selective targeting of PKM2 may provide a new strategy for cancer therapy, especially for patients with therapeutic resistance.
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April-2017
Volume 37 Issue 4

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Guo C, Yan C, Luo L, Goto S, Urata Y, Xu J, Wen X, Kuang Y, Tou F, Li T, Li T, et al: Enhanced expression of PKM2 associates with the biological properties of cancer stem cells from A549 human lung cancer cells. Oncol Rep 37: 2161-2166, 2017
APA
Guo, C., Yan, C., Luo, L., Goto, S., Urata, Y., Xu, J. ... Li, T. (2017). Enhanced expression of PKM2 associates with the biological properties of cancer stem cells from A549 human lung cancer cells. Oncology Reports, 37, 2161-2166. https://doi.org/10.3892/or.2017.5438
MLA
Guo, C., Yan, C., Luo, L., Goto, S., Urata, Y., Xu, J., Wen, X., Kuang, Y., Tou, F., Li, T."Enhanced expression of PKM2 associates with the biological properties of cancer stem cells from A549 human lung cancer cells". Oncology Reports 37.4 (2017): 2161-2166.
Chicago
Guo, C., Yan, C., Luo, L., Goto, S., Urata, Y., Xu, J., Wen, X., Kuang, Y., Tou, F., Li, T."Enhanced expression of PKM2 associates with the biological properties of cancer stem cells from A549 human lung cancer cells". Oncology Reports 37, no. 4 (2017): 2161-2166. https://doi.org/10.3892/or.2017.5438