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miR-30a radiosensitizes non-small cell lung cancer by targeting ATF1 that is involved in the phosphorylation of ATM

  • Authors:
    • Yuyan Guo
    • Wenze Sun
    • Tuotuo Gong
    • Yanlan Chai
    • Juan Wang
    • Beina Hui
    • Yi Li
    • Liping Song
    • Ying Gao
  • View Affiliations / Copyright

    Affiliations: Department of Radiation Oncology, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710061, P.R. China
    Copyright: © Guo et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1980-1988
    |
    Published online on: February 14, 2017
       https://doi.org/10.3892/or.2017.5448
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Abstract

Increasing number of studies report that microRNAs play important roles in radiosensitization. miR-30a has been proved to perform many functions in the development and treatment of cancer, and it is downregulated in non-small cell lung cancer (NSCLC) tissues and cells. This study was conducted to understand if miR-30a plays a role in the radiosensitivity of NSCLC cells. Radiosensitivity was examed by colony survival assay and tumor volume changing in vitro and in vivo, respectively. Bioinformatic analysis and luciferase reporter assays were used to distinguish the candidate target of miR-30a. qRT-PCR and western blotting were carried out to detect the relative expression of mRNAs and proteins. Cell cycle and cell apoptosis were determined by flow cytometry. Our results illustrated miR-30a could increase the radiosensitivity of NSCLC, especially in A549 cell line. In vivo experiment also showed the potential radiosensitizing possibility of miR-30a. Further exploration validated that miR-30a was directly targ­eting activating transcription factor 1 (ATF1). In studying the ataxia-telangiectasia mutated (ATM) associated effects on cell radiosensitivity, we found that miR-30a could reduce radiation induced G2/M cell cycle arrest and may also affect radiation induced apoptosis. Together, our results demonstrated that miR-30a may modulate the radiosensitivity of NSCLC through reducing the function of ATF1 in phosphorylation of ATM and have potential therapeutic value.
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Copy and paste a formatted citation
Spandidos Publications style
Guo Y, Sun W, Gong T, Chai Y, Wang J, Hui B, Li Y, Song L and Gao Y: miR-30a radiosensitizes non-small cell lung cancer by targeting ATF1 that is involved in the phosphorylation of ATM. Oncol Rep 37: 1980-1988, 2017.
APA
Guo, Y., Sun, W., Gong, T., Chai, Y., Wang, J., Hui, B. ... Gao, Y. (2017). miR-30a radiosensitizes non-small cell lung cancer by targeting ATF1 that is involved in the phosphorylation of ATM. Oncology Reports, 37, 1980-1988. https://doi.org/10.3892/or.2017.5448
MLA
Guo, Y., Sun, W., Gong, T., Chai, Y., Wang, J., Hui, B., Li, Y., Song, L., Gao, Y."miR-30a radiosensitizes non-small cell lung cancer by targeting ATF1 that is involved in the phosphorylation of ATM". Oncology Reports 37.4 (2017): 1980-1988.
Chicago
Guo, Y., Sun, W., Gong, T., Chai, Y., Wang, J., Hui, B., Li, Y., Song, L., Gao, Y."miR-30a radiosensitizes non-small cell lung cancer by targeting ATF1 that is involved in the phosphorylation of ATM". Oncology Reports 37, no. 4 (2017): 1980-1988. https://doi.org/10.3892/or.2017.5448
Copy and paste a formatted citation
x
Spandidos Publications style
Guo Y, Sun W, Gong T, Chai Y, Wang J, Hui B, Li Y, Song L and Gao Y: miR-30a radiosensitizes non-small cell lung cancer by targeting ATF1 that is involved in the phosphorylation of ATM. Oncol Rep 37: 1980-1988, 2017.
APA
Guo, Y., Sun, W., Gong, T., Chai, Y., Wang, J., Hui, B. ... Gao, Y. (2017). miR-30a radiosensitizes non-small cell lung cancer by targeting ATF1 that is involved in the phosphorylation of ATM. Oncology Reports, 37, 1980-1988. https://doi.org/10.3892/or.2017.5448
MLA
Guo, Y., Sun, W., Gong, T., Chai, Y., Wang, J., Hui, B., Li, Y., Song, L., Gao, Y."miR-30a radiosensitizes non-small cell lung cancer by targeting ATF1 that is involved in the phosphorylation of ATM". Oncology Reports 37.4 (2017): 1980-1988.
Chicago
Guo, Y., Sun, W., Gong, T., Chai, Y., Wang, J., Hui, B., Li, Y., Song, L., Gao, Y."miR-30a radiosensitizes non-small cell lung cancer by targeting ATF1 that is involved in the phosphorylation of ATM". Oncology Reports 37, no. 4 (2017): 1980-1988. https://doi.org/10.3892/or.2017.5448
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