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Article

Absence of Malat1 does not prevent DEN-induced hepatocarcinoma in mice

  • Authors:
    • Stéphanie Miard
    • Marie-Josée Girard
    • Philippe Joubert
    • Sophie Carter
    • Amy Gonzales
    • Huiping Guo
    • Benjamin Morpurgo
    • Louise Boivin
    • Andrei Golovko
    • Frédéric Picard
  • View Affiliations / Copyright

    Affiliations: Research Center, Quebec Heart and Lung Institute, Laval University, Québec, QC G1V 4G5, Canada, Texas A&M Institute for Genomic Medicine, College Station, TX 77843, USA
  • Pages: 2153-2160
    |
    Published online on: February 20, 2017
       https://doi.org/10.3892/or.2017.5468
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Abstract

Long non-coding RNAs (lncRNA) are key regulators of gene expression both at the transcriptional and post-transcriptional levels. The lncRNA metastasis associated lung adenocarcinoma transcript 1 (Malat1) is overexpressed in many types of cancer, including hepatocarcinoma, and induces cell proliferation in several cell lines in vitro. However, the direct causal effects of Malat1 on hepatocyte proliferation and liver carcinogenesis in vivo are not fully understood. To better determine the contribution of Malat1 to hepatocarcinoma oncogenesis, this study was aimed at testing the hypothesis that its absence confers resistance to the development of liver tumors. Male Malat1-/- mice and their wild-type (WT) littermates were studied one year after treatment with the genotoxic agent diethylnitrosamine (DEN), a potent inducer of liver cancer. As expected, in WT mice, Malat1 expression was significantly higher in hepatic tumors than in healthy liver regions. Altered hepatic mRNA levels of Ki67, HDAC3, NFκB and p27 were observed in DEN-treated Malat1-/- mice. Despite this, these mice were characterized by similar liver weight, prevalence of tumors, and histological features compared to those of their WT littermates. In parallel, plasma lipids and glucose homeostasis did not significantly differ between DEN-treated groups. These findings support a role for Malat1 as a marker of liver carcinogenesis, but also suggest that its role in the regulation of hepatocyte hyperproliferation in mice is either minimal or masked by redundant and/or overwhelming mechanisms.
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Copy and paste a formatted citation
Spandidos Publications style
Miard S, Girard M, Joubert P, Carter S, Gonzales A, Guo H, Morpurgo B, Boivin L, Golovko A, Picard F, Picard F, et al: Absence of Malat1 does not prevent DEN-induced hepatocarcinoma in mice. Oncol Rep 37: 2153-2160, 2017.
APA
Miard, S., Girard, M., Joubert, P., Carter, S., Gonzales, A., Guo, H. ... Picard, F. (2017). Absence of Malat1 does not prevent DEN-induced hepatocarcinoma in mice. Oncology Reports, 37, 2153-2160. https://doi.org/10.3892/or.2017.5468
MLA
Miard, S., Girard, M., Joubert, P., Carter, S., Gonzales, A., Guo, H., Morpurgo, B., Boivin, L., Golovko, A., Picard, F."Absence of Malat1 does not prevent DEN-induced hepatocarcinoma in mice". Oncology Reports 37.4 (2017): 2153-2160.
Chicago
Miard, S., Girard, M., Joubert, P., Carter, S., Gonzales, A., Guo, H., Morpurgo, B., Boivin, L., Golovko, A., Picard, F."Absence of Malat1 does not prevent DEN-induced hepatocarcinoma in mice". Oncology Reports 37, no. 4 (2017): 2153-2160. https://doi.org/10.3892/or.2017.5468
Copy and paste a formatted citation
x
Spandidos Publications style
Miard S, Girard M, Joubert P, Carter S, Gonzales A, Guo H, Morpurgo B, Boivin L, Golovko A, Picard F, Picard F, et al: Absence of Malat1 does not prevent DEN-induced hepatocarcinoma in mice. Oncol Rep 37: 2153-2160, 2017.
APA
Miard, S., Girard, M., Joubert, P., Carter, S., Gonzales, A., Guo, H. ... Picard, F. (2017). Absence of Malat1 does not prevent DEN-induced hepatocarcinoma in mice. Oncology Reports, 37, 2153-2160. https://doi.org/10.3892/or.2017.5468
MLA
Miard, S., Girard, M., Joubert, P., Carter, S., Gonzales, A., Guo, H., Morpurgo, B., Boivin, L., Golovko, A., Picard, F."Absence of Malat1 does not prevent DEN-induced hepatocarcinoma in mice". Oncology Reports 37.4 (2017): 2153-2160.
Chicago
Miard, S., Girard, M., Joubert, P., Carter, S., Gonzales, A., Guo, H., Morpurgo, B., Boivin, L., Golovko, A., Picard, F."Absence of Malat1 does not prevent DEN-induced hepatocarcinoma in mice". Oncology Reports 37, no. 4 (2017): 2153-2160. https://doi.org/10.3892/or.2017.5468
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