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Article

Actin-like protein 6A is a novel prognostic indicator promoting invasion and metastasis in osteosarcoma

  • Authors:
    • Wei Sun
    • Wanchun Wang
    • Jian Lei
    • Hui Li
    • Yi Wu
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedics Surgery, The Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, P.R. China, Department of Pathology, The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Changsha, Hunan 410013, P.R. China
  • Pages: 2405-2417
    |
    Published online on: February 23, 2017
       https://doi.org/10.3892/or.2017.5473
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Abstract

Osteosarcoma harbors highly metastatic properties, accounting for postoperative recurrence and metastasis. Actin-like protein 6A (ACTL6A) regulates cell proliferation, migration and differentiation. However, the biologic role of ACTL6A in osteosarcoma remains unknown. In this study, the results showed that, by analysis of frozen fresh primary tumor tissues, matched non-cancerous bone tissues (NCBTs) and biopsy lung metastatic nodule tissues from 30 osteosarcoma patients after radical surgical resection, ACTL6A was overexpressed in osteosarcoma tissues compared with matched NCBTs, and its expression level was associated with osteosarcoma metastasis. Immunohistochemical analyses of osteosarcoma tissue samples from two independent cohorts of formaldehyde-fixed, paraffin-embedded osteosarcoma tissue samples from total of 186 osteosarcoma patients showed that high ACTL6A expression correlated with malignant clinicopathological features such as larger tumor size, high Ennecking grade, high histologic grade, and advanced tumor node metastasis stage. High ACTL6A expression was associated with poor prognosis for patients with osteosarcoma, and an independent and significant risk factor for disease-free survival and overall survival after radical tumor resection. Both in vitro and in vivo assays showed that ACTL6A overexpression promoted osteosarcoma cell invasion and metastasis, whereas knockdown of ACTL6A expression reduced osteosarcoma cell malignant behavior such as invasion and metastasis. Furthermore, we proved that ACTL6A promoted osteosarcoma cells of metastasis through facilitating epithelial-mesenchymal transition (EMT). In conclusion, data from the present study demonstrated that ACTL6A was associated with poor survival and promoted osteosarcoma cell metastasis through EMT, suggesting that ACTL6A may be a novel prognostic biomarker and therapeutic target for osteosarcoma.
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View References

1 

Mirabello L, Troisi RJ and Savage SA: International osteosarcoma incidence patterns in children and adolescents, middle ages and elderly persons. Int J Cancer. 125:229–234. 2009. View Article : Google Scholar : PubMed/NCBI

2 

Ritter J and Bielack SS: Osteosarcoma. Ann Oncol. 21:(Suppl 7). vii320–vii325. 2010. View Article : Google Scholar : PubMed/NCBI

3 

Isakoff MS, Bielack SS, Meltzer P and Gorlick R: Osteosarcoma: current treatment and a collaborative pathway to success. J Clin Oncol. 33:3029–3035. 2015. View Article : Google Scholar : PubMed/NCBI

4 

Klein MJ and Siegal GP: Osteosarcoma: anatomic and histologic variants. Am J Clin Pathol. 125:555–581. 2006. View Article : Google Scholar : PubMed/NCBI

5 

Ren HY, Sun LL, Li HY and Ye ZM: Prognostic significance of serum alkaline phosphatase level in osteosarcoma: a meta-analysis of published data. BioMed Res Int. 2015:1608352015. View Article : Google Scholar : PubMed/NCBI

6 

Zhang Y, Zhang L, Zhang G, Li S, Duan J, Cheng J, Ding G, Zhou C, Zhang J, Luo P, et al: Osteosarcoma metastasis: prospective role of ezrin. Tumour Biol. 35:5055–5059. 2014. View Article : Google Scholar : PubMed/NCBI

7 

Scott MC, Sarver AL, Tomiyasu H, Cornax I, Van Etten J, Varshney J, O'Sullivan MG, Subramanian S and Modiano JF: Aberrant retinoblastoma (RB)-E2F transcriptional regulation defines molecular phenotypes of osteosarcoma. J Biol Chem. 290:28070–28083. 2015. View Article : Google Scholar : PubMed/NCBI

8 

Del Mare S, Husanie H, Iancu O, Abu-Odeh M, Evangelou K, Lovat F, Volinia S, Gordon J, Amir G, Stein J, et al: WWOX and p53 dysregulation synergize to drive the development of osteosarcoma. Cancer Res. 76:6107–6117. 2016. View Article : Google Scholar : PubMed/NCBI

9 

Aguirre-Ghiso JA: Models, mechanisms and clinical evidence for cancer dormancy. Nat Rev Cancer. 7:834–846. 2007. View Article : Google Scholar : PubMed/NCBI

10 

Yang G, Yuan J and Li K: EMT transcription factors: implication in osteosarcoma. Med Oncol. 30:6972013. View Article : Google Scholar : PubMed/NCBI

11 

Hou CH, Lin FL, Hou SM and Liu JF: Cyr61 promotes epithelial-mesenchymal transition and tumor metastasis of osteosarcoma by Raf-1/MEK/ERK/Elk-1/TWIST-1 signaling pathway. Mol Cancer. 13:2362014. View Article : Google Scholar : PubMed/NCBI

12 

Zhao K, Wang W, Rando OJ, Xue Y, Swiderek K, Kuo A and Crabtree GR: Rapid and phosphoinositol-dependent binding of the SWI/SNF-like BAF complex to chromatin after T lymphocyte receptor signaling. Cell. 95:625–636. 1998. View Article : Google Scholar : PubMed/NCBI

13 

Krasteva V, Buscarlet M, Diaz-Tellez A, Bernard MA, Crabtree GR and Lessard JA: The BAF53a subunit of SWI/SNF-like BAF complexes is essential for hemopoietic stem cell function. Blood. 120:4720–4732. 2012. View Article : Google Scholar : PubMed/NCBI

14 

Lessard J, Wu JI, Ranish JA, Wan M, Winslow MM, Staahl BT, Wu H, Aebersold R, Graef IA and Crabtree GR: An essential switch in subunit composition of a chromatin remodeling complex during neural development. Neuron. 55:201–215. 2007. View Article : Google Scholar : PubMed/NCBI

15 

Nguyen KH, Xu F, Flowers S, Williams EA, Fritton JC and Moran E: SWI/SNF-mediated lineage determination in mesenchymal stem cells confers resistance to osteoporosis. Stem Cells. 33:3028–3038. 2015. View Article : Google Scholar : PubMed/NCBI

16 

Bao X, Tang J, Lopez-Pajares V, Tao S, Qu K, Crabtree GR and Khavari PA: ACTL6a enforces the epidermal progenitor state by suppressing SWI/SNF-dependent induction of KLF4. Cell Stem Cell. 12:193–203. 2013. View Article : Google Scholar : PubMed/NCBI

17 

Lu W, Fang L, Ouyang B, Zhang X, Zhan S, Feng X, Bai Y, Han X, Kim H, He Q, et al: Actl6a protects embryonic stem cells from differentiating into primitive endoderm. Stem Cells. 33:1782–1793. 2015. View Article : Google Scholar : PubMed/NCBI

18 

Park J, Wood MA and Cole MD: BAF53 forms distinct nuclear complexes and functions as a critical c-Myc-interacting nuclear cofactor for oncogenic transformation. Mol Cell Biol. 22:1307–1316. 2002. View Article : Google Scholar : PubMed/NCBI

19 

Zhao D, Pan C, Sun J, Gilbert C, Drews-Elger K, Azzam DJ, Picon-Ruiz M, Kim M, Ullmer W, El-Ashry D, et al: VEGF drives cancer-initiating stem cells through VEGFR-2/Stat3 signaling to upregulate Myc and Sox2. Oncogene. 34:3107–3119. 2015. View Article : Google Scholar : PubMed/NCBI

20 

Yong KJ, Gao C, Lim JS, Yan B, Yang H, Dimitrov T, Kawasaki A, Ong CW, Wong KF, Lee S, et al: Oncofetal gene SALL4 in aggressive hepatocellular carcinoma. N Engl J Med. 368:2266–2276. 2013. View Article : Google Scholar : PubMed/NCBI

21 

Pullar CE, Chen J and Isseroff RR: PP2A activation by beta2-adrenergic receptor agonists: novel regulatory mechanism of keratinocyte migration. J Biol Chem. 278:22555–22562. 2003. View Article : Google Scholar : PubMed/NCBI

22 

Fang F, Chang RM, Yu L, Lei X, Xiao S, Yang H and Yang LY: MicroRNA-188-5p suppresses tumor cell proliferation and metastasis by directly targeting FGF5 in hepatocellular carcinoma. J Hepatol. 63:874–885. 2015. View Article : Google Scholar : PubMed/NCBI

23 

Su S, Liu Q, Chen J, Chen J, Chen F, He C, Huang D, Wu W, Lin L, Huang W, et al: A positive feedback loop between mesenchymal-like cancer cells and macrophages is essential to breast cancer metastasis. Cancer Cell. 25:605–620. 2014. View Article : Google Scholar : PubMed/NCBI

24 

Qiang L and He YY: Autophagy deficiency stabilizes TWIST1 to promote epithelial-mesenchymal transition. Autophagy. 10:1864–1865. 2014. View Article : Google Scholar : PubMed/NCBI

25 

Horvai AE, Roy R, Borys D and O'Donnell RJ: Regulators of skeletal development: a cluster analysis of 206 bone tumors reveals diagnostically useful markers. Mod Pathol. 25:1452–1461. 2012. View Article : Google Scholar : PubMed/NCBI

26 

Kempf-Bielack B, Bielack SS, Jürgens H, Branscheid D, Berdel WE, Exner GU, Göbel U, Helmke K, Jundt G, Kabisch H, et al: Osteosarcoma relapse after combined modality therapy: an analysis of unselected patients in the Cooperative Osteosarcoma Study Group (COSS). J Clin Oncol. 23:559–568. 2005. View Article : Google Scholar : PubMed/NCBI

27 

Lee K, Shim JH, Kang MJ, Kim JH, Ahn JS, Yoo SJ, Kwon Kim Y and Kwon H: Association of BAF53 with mitotic chromosomes. Mol Cells. 24:288–293. 2007.PubMed/NCBI

28 

Ferrari S, Bertoni F, Mercuri M, Picci P, Giacomini S, Longhi A and Bacci G: Predictive factors of disease-free survival for non-metastatic osteosarcoma of the extremity: an analysis of 300 patients treated at the Rizzoli Institute. Ann Oncol. 12:1145–1150. 2001. View Article : Google Scholar : PubMed/NCBI

29 

Xiao S, Chang RM, Yang MY, Lei X, Liu X, Gao WB, Xiao JL and Yang LY: Actin-like 6A predicts poor prognosis of hepatocellular carcinoma and promotes metastasis and epithelial-mesenchymal transition. Hepatology. 63:1256–1271. 2016. View Article : Google Scholar : PubMed/NCBI

30 

Thiery JP, Acloque H, Huang RY and Nieto MA: Epithelial- mesenchymal transitions in development and disease. Cell. 139:871–890. 2009. View Article : Google Scholar : PubMed/NCBI

31 

Huang W, Chen Z, Shang X, Tian D, Wang D, Wu K, Fan D and Xia L: Sox12, a direct target of FoxQ1, promotes hepatocellular carcinoma metastasis through up-regulating Twist1 and FGFBP1. Hepatology. 61:1920–1933. 2015. View Article : Google Scholar : PubMed/NCBI

32 

Hsu KW, Hsieh RH, Huang KH, Li Fen-Yau A, Chi CW, Wang TY, Tseng MJ, Wu KJ and Yeh TS: Activation of the Notch1/STAT3/Twist signaling axis promotes gastric cancer progression. Carcinogenesis. 33:1459–1467. 2012. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Sun W, Wang W, Lei J, Li H and Wu Y: Actin-like protein 6A is a novel prognostic indicator promoting invasion and metastasis in osteosarcoma. Oncol Rep 37: 2405-2417, 2017.
APA
Sun, W., Wang, W., Lei, J., Li, H., & Wu, Y. (2017). Actin-like protein 6A is a novel prognostic indicator promoting invasion and metastasis in osteosarcoma. Oncology Reports, 37, 2405-2417. https://doi.org/10.3892/or.2017.5473
MLA
Sun, W., Wang, W., Lei, J., Li, H., Wu, Y."Actin-like protein 6A is a novel prognostic indicator promoting invasion and metastasis in osteosarcoma". Oncology Reports 37.4 (2017): 2405-2417.
Chicago
Sun, W., Wang, W., Lei, J., Li, H., Wu, Y."Actin-like protein 6A is a novel prognostic indicator promoting invasion and metastasis in osteosarcoma". Oncology Reports 37, no. 4 (2017): 2405-2417. https://doi.org/10.3892/or.2017.5473
Copy and paste a formatted citation
x
Spandidos Publications style
Sun W, Wang W, Lei J, Li H and Wu Y: Actin-like protein 6A is a novel prognostic indicator promoting invasion and metastasis in osteosarcoma. Oncol Rep 37: 2405-2417, 2017.
APA
Sun, W., Wang, W., Lei, J., Li, H., & Wu, Y. (2017). Actin-like protein 6A is a novel prognostic indicator promoting invasion and metastasis in osteosarcoma. Oncology Reports, 37, 2405-2417. https://doi.org/10.3892/or.2017.5473
MLA
Sun, W., Wang, W., Lei, J., Li, H., Wu, Y."Actin-like protein 6A is a novel prognostic indicator promoting invasion and metastasis in osteosarcoma". Oncology Reports 37.4 (2017): 2405-2417.
Chicago
Sun, W., Wang, W., Lei, J., Li, H., Wu, Y."Actin-like protein 6A is a novel prognostic indicator promoting invasion and metastasis in osteosarcoma". Oncology Reports 37, no. 4 (2017): 2405-2417. https://doi.org/10.3892/or.2017.5473
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