miR-92a promotes tumor growth of osteosarcoma by targeting PTEN/AKT signaling pathway

  • Authors:
    • Jie Xiao
    • Weifeng Yu
    • Kongzu Hu
    • Maoqiang Li
    • Jianwei Chen
    • Zhanchun Li
  • View Affiliations

  • Published online on: March 2, 2017     https://doi.org/10.3892/or.2017.5484
  • Pages: 2513-2521
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Abstract

MicroRNAs (miRNAs) play critical roles in human cancers including osteosarcoma (OS). miR-92a has been found to be a cancer-related miRNA in many cancer types and it is upregulated in OS cell lines. However, the expression and biological function of miR-92a in OS have not been investigated. In this study, we showed that miR-92a expression was increased in OS tissues, and its high expression was correlated with clinical stage, T classification and histological differentiation. Furthermore, patients with high expression of miR-92a had a significantly poorer survival rate. Functionally, miR-92a overexpression promoted the proliferation and cell cycle progression, and inhibited apoptosis in MG-63 cells. While inhibition of miR-92a showed contrary effects with reduced proliferation, cell cycle arrest at G1 phase and increased apoptosis in U2OS cells. Moreover, we confirmed that miR-92a inhibition reversed the tumor growth of OS cells in nude mice. Phosphatase and tensin homolog (PTEN), a well-known tumor suppressor, was confirmed to be the direct downstream target of miR-92a in OS. Notably, miR-92a consequently regulated the expression of the downstream targets of PTEN/AKT signaling pathway including p-Akt(Ser473), mTOR, p-p27(Thr157) and p-MDM2(Ser166). Furthermore, PTEN knockdown abrogated the functional effects of miR-92a silencing on the proliferation, apoptosis and cell cycle progression in OS cells. Thus, miR-92a that exerts an oncogenic role by targeting PTEN/AKT pathway in OS potentially acts as a biomarker and drug-target.
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April-2017
Volume 37 Issue 4

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Xiao J, Yu W, Hu K, Li M, Chen J and Li Z: miR-92a promotes tumor growth of osteosarcoma by targeting PTEN/AKT signaling pathway. Oncol Rep 37: 2513-2521, 2017
APA
Xiao, J., Yu, W., Hu, K., Li, M., Chen, J., & Li, Z. (2017). miR-92a promotes tumor growth of osteosarcoma by targeting PTEN/AKT signaling pathway. Oncology Reports, 37, 2513-2521. https://doi.org/10.3892/or.2017.5484
MLA
Xiao, J., Yu, W., Hu, K., Li, M., Chen, J., Li, Z."miR-92a promotes tumor growth of osteosarcoma by targeting PTEN/AKT signaling pathway". Oncology Reports 37.4 (2017): 2513-2521.
Chicago
Xiao, J., Yu, W., Hu, K., Li, M., Chen, J., Li, Z."miR-92a promotes tumor growth of osteosarcoma by targeting PTEN/AKT signaling pathway". Oncology Reports 37, no. 4 (2017): 2513-2521. https://doi.org/10.3892/or.2017.5484