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Article

MicroRNA-134 suppresses cell proliferation in gastric cancer cells via targeting of GOLPH3

  • Authors:
    • Yanqi Liu
    • Yanhong Sun
    • Aizhen Zhao
  • View Affiliations / Copyright

    Affiliations: Department of Gastroenterology, The Affiliated Hospital of Inner Mongolia Medical University, Hohhot, Inner Mongolia 010050, P.R. China, Department of Physiology, Inner Mongolia Medical University, Hohhot, Inner Mongolia 010050, P.R. China, Department of Ultrasound, The Affiliated Hospital of Inner Mongolia Medical University, Hohhot, Inner Mongolia 010050, P.R. China
  • Pages: 2441-2448
    |
    Published online on: March 2, 2017
       https://doi.org/10.3892/or.2017.5488
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Abstract

Emerging evidence has shown that microRNAs (miRNAs) play critical roles in tumor development and progression. miR-134 has been found to act as a tumor-suppressor in numerous types of cancers. However, little is known concerning the potential role of miR-134 in gastric carcinogenesis. In the present study, we found that miR-134 was highly downregulated in gastric cancer tissues and cell lines when compared with levels in their adjacent non-tumor tissues and the normal human gastric epithelial cell line GES-1. Additionally, overexpression of miR-134 was accompanied by reduced cell proliferation in vitro and decreased tumor size in vivo. Further investigation by luciferase reporter assay indicated that Golgi phosphoprotein 3 (GOLPH3), a potent oncogene, was a direct target of miR-134. The activity of a luciferase reporter carrying the miR-134 binding site in the 3'-untranslated region (3'-UTR) of GOLPH3 was repressed by overexpression of miR-134, while a mutation in the 3'-UTR of GOLPH3 abrogated this effect, indicating that GOLPH3 is a target gene of miR-134. Overexpression of GOLPH3 blocked the antiproliferative effect of pre-miR-134 in gastric carcinoma cells. Furthermore, overexpression of miR-134 was associated with decreased phosphorylation of AKT, mTOR and S6K. Taken together, these data suggest that miR-134 regulates gastric cancer cell proliferation, at least potentially, through downregulation of the GOLPH3 gene, implicating a candidate tumor-suppressor miRNA in the pathogenesis of gastric cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Liu Y, Sun Y and Zhao A: MicroRNA-134 suppresses cell proliferation in gastric cancer cells via targeting of GOLPH3. Oncol Rep 37: 2441-2448, 2017.
APA
Liu, Y., Sun, Y., & Zhao, A. (2017). MicroRNA-134 suppresses cell proliferation in gastric cancer cells via targeting of GOLPH3. Oncology Reports, 37, 2441-2448. https://doi.org/10.3892/or.2017.5488
MLA
Liu, Y., Sun, Y., Zhao, A."MicroRNA-134 suppresses cell proliferation in gastric cancer cells via targeting of GOLPH3". Oncology Reports 37.4 (2017): 2441-2448.
Chicago
Liu, Y., Sun, Y., Zhao, A."MicroRNA-134 suppresses cell proliferation in gastric cancer cells via targeting of GOLPH3". Oncology Reports 37, no. 4 (2017): 2441-2448. https://doi.org/10.3892/or.2017.5488
Copy and paste a formatted citation
x
Spandidos Publications style
Liu Y, Sun Y and Zhao A: MicroRNA-134 suppresses cell proliferation in gastric cancer cells via targeting of GOLPH3. Oncol Rep 37: 2441-2448, 2017.
APA
Liu, Y., Sun, Y., & Zhao, A. (2017). MicroRNA-134 suppresses cell proliferation in gastric cancer cells via targeting of GOLPH3. Oncology Reports, 37, 2441-2448. https://doi.org/10.3892/or.2017.5488
MLA
Liu, Y., Sun, Y., Zhao, A."MicroRNA-134 suppresses cell proliferation in gastric cancer cells via targeting of GOLPH3". Oncology Reports 37.4 (2017): 2441-2448.
Chicago
Liu, Y., Sun, Y., Zhao, A."MicroRNA-134 suppresses cell proliferation in gastric cancer cells via targeting of GOLPH3". Oncology Reports 37, no. 4 (2017): 2441-2448. https://doi.org/10.3892/or.2017.5488
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