Identification of novel cancer fusion genes using chromosome breakpoint screening

  • Authors:
    • Kate Hua
    • Chin-Hui Lin
    • Ya-Lun Chen
    • Chi-Hung Lin
    • Yueh‑Hsin Ping
    • Yuh-Shan Jou
    • Chian-Feng Chen
  • View Affiliations

  • Published online on: March 6, 2017     https://doi.org/10.3892/or.2017.5492
  • Pages: 2101-2108
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Abstract

Gene fusion due to rearrangement or translocation of chromosomes is a powerful mutational mechanism during tumorigenesis. Several new high-resolution technologies have recently been developed to evaluate large numbers of small aberrations as candidate loci for fusion gene screening. In our previous whole-genome screening study using 500K SNP arrays, we identified more than 700 homozygous deletions (HDs) and amplicons in 23 cancer cell lines. To explore novel fusion genes in cancer, we established stringent criteria for defining HD and amplicon breakpoints. Then genomic PCR and sequencing analyses identified a fusion gene, FNDC3B-PRKCI, that resulted from chromosome intra-rearrangement. Western blotting and 3'-RACE analyses revealed that the chimeric transcript was an in-frame fusion between FNDC3B and PRKCI. Finally, cell migration and colony formation assays suggested that FNDC3B-PRKCI is a potential oncogene.
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April-2017
Volume 37 Issue 4

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Hua K, Lin C, Chen Y, Lin C, Ping YH, Jou Y and Chen C: Identification of novel cancer fusion genes using chromosome breakpoint screening. Oncol Rep 37: 2101-2108, 2017
APA
Hua, K., Lin, C., Chen, Y., Lin, C., Ping, Y., Jou, Y., & Chen, C. (2017). Identification of novel cancer fusion genes using chromosome breakpoint screening. Oncology Reports, 37, 2101-2108. https://doi.org/10.3892/or.2017.5492
MLA
Hua, K., Lin, C., Chen, Y., Lin, C., Ping, Y., Jou, Y., Chen, C."Identification of novel cancer fusion genes using chromosome breakpoint screening". Oncology Reports 37.4 (2017): 2101-2108.
Chicago
Hua, K., Lin, C., Chen, Y., Lin, C., Ping, Y., Jou, Y., Chen, C."Identification of novel cancer fusion genes using chromosome breakpoint screening". Oncology Reports 37, no. 4 (2017): 2101-2108. https://doi.org/10.3892/or.2017.5492