Runx2 downregulation, migration and proliferation inhibition in melanoma cells treated with BEL β-trefoil

Perduca,Massimiliano; Dalle Carbonare,Luca; Bovi,Michele; Innamorati,Giulio; Cheri,Samuele; Cavallini,Chiara; Scupoli,Maria Teresa; Mori,Antonio; Valenti,Maria Teresa

doi:10.3892/or.2017.5493

April-2017 Volume 37 Issue 4

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April-2017 Volume 37 Issue 4

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Article

Runx2 downregulation, migration and proliferation inhibition in melanoma cells treated with BEL β-trefoil

Authors:
- Massimiliano Perduca
- Luca Dalle Carbonare
- Michele Bovi
- Giulio Innamorati
- Samuele Cheri
- Chiara Cavallini
- Maria Teresa Scupoli
- Antonio Mori
- Maria Teresa Valenti
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Affiliations: Department of Biotechnology, Biocrystallography Laboratory, University of Verona, I-37134 Verona, Italy, Department of Medicine, Clinic of Internal Medicine, Section D, University of Verona, I-37134 Verona, Italy, University Laboratory for Medical Research (LURM), University of Verona, I-37134 Verona, Italy
Pages: 2209-2214
|
Published online on: March 6, 2017

https://doi.org/10.3892/or.2017.5493
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Abstract

Malignant melanoma is a lethal form of skin cancer and highly metastatic tumor with poor prognosis; BEL β-trefoil, a lectin, obtained by our group, possesses the ability to act specifically on malignant cells. Therefore, the aim of our study was to investigate the effects of BEL β-trefoil in melanoma cells in an attempt to evaluate its potential usage as anticancer agent. BEL β-trefoil was purified by chromatography and A375 and MeWo melanoma cells were treated. Viability and proliferation were evaluated as well as apoptosis, RUNX2 gene expression and migration ability. The treated tumor cells decreased viability as well as proliferative ability. Flow cytometry analysis showed a lessen effect of the treatment on apoptosis. The gene expression analysis showed a reduction of RUNX2 expression in a dose-dependent manner and migration ability was reduced significantly in both treated cell lines. Our findings suggest that BEL β-trefoil can be considered a useful tool against malignancy due to its effect based on the simultaneous proliferation ability reduction as well as the inhibition of migration capacity on melanoma tumor cells.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Runx2 downregulation, migration and proliferation inhibition in melanoma cells treated with BEL β-trefoil

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