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G protein subunit α q regulates gastric cancer growth via the p53/p21 and MEK/ERK pathways

  • Authors:
    • Yizhuo Wang
    • Huijie Xiao
    • Haitao Wu
    • Cheng Yao
    • Hua He
    • Chang Wang
    • Wei Li
  • View Affiliations / Copyright

    Affiliations: Cancer Center, First Hospital of Jilin University, Changchun, Jilin 130021, P.R. China, Department of Gastrointestinal Colorectal and Anal Surgery, China Japan Union Hospital of Jilin University, Changchun, Jilin 130033, P.R. China
    Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1998-2006
    |
    Published online on: March 13, 2017
       https://doi.org/10.3892/or.2017.5500
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Abstract

Genetic alterations in G protein subunit α q (GNAQ) have been reported in numerous types of human cancer. However, the role of GNAQ in human gastric cancer (GC) has not been explored. In the present study, we found that GNAQ was highly expressed in GC patient samples and GNAQ expression was related to patient age, GC differentiation status and adjuvant therapy, as determined by immunohistochemical assay. Lentivirus delivery of short hairpin RNA (shRNA) targeting GNAQ was used to explore the function of GNAQ in GC cells. Silencing of GNAQ markedly suppressed proliferation and colony formation in GC cells, and arrested the cell cycle at the S phase. Mechanistic analysis revealed that knockdown of GNAQ significantly increased the expression of p53 and p21, and decreased cyclin A and p-CDK2 protein expression. Moreover, the phosphorylation of ERK and MEK was also decreased after knockdown of GNAQ as determined by western blotting assay. Overall, our results suggest that GNAQ plays a critical role in regulating GC cell growth and survival via canonical oncogenic signaling pathways including MAPK and p53, and therefore serves as a promising new therapeutic target in GC.
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1 

Ferlay J, Soerjomataram I, Dikshit R, Eser S, Mathers C, Rebelo M, Parkin DM, Forman D and Bray F: Cancer incidence and mortality worldwide: Sources, methods and major patterns in GLOBOCAN 2012. Int J Cancer. 136:E359–E386. 2015. View Article : Google Scholar : PubMed/NCBI

2 

Harder J and Opitz OG: Gastric cancer - risk factors and medical therapy. Praxis. 95:1021–1028. 2006.(In German). View Article : Google Scholar : PubMed/NCBI

3 

Fuchs CS, Tomasek J, Yong CJ, Dumitru F, Passalacqua R, Goswami C, Safran H, dos Santos LV, Aprile G, Ferry DR, et al: REGARD Trial Investigators: Ramucirumab monotherapy for previously treated advanced gastric or gastro-oesophageal junction adenocarcinoma (REGARD): An international, randomised, multicentre, placebo-controlled, phase 3 trial. Lancet. 383:31–39. 2014. View Article : Google Scholar : PubMed/NCBI

4 

Ligtenberg MJ, Kuiper RP, Chan TL, Goossens M, Hebeda KM, Voorendt M, Lee TY, Bodmer D, Hoenselaar E, Hendriks-Cornelissen SJ, et al: Heritable somatic methylation and inactivation of MSH2 in families with Lynch syndrome due to deletion of the 3′ exons of TACSTD1. Nat Genet. 41:112–117. 2009. View Article : Google Scholar : PubMed/NCBI

5 

Bang YJ, Van Cutsem E, Feyereislova A, Chung HC, Shen L, Sawaki A, Lordick F, Ohtsu A, Omuro Y, Satoh T, et al: ToGA Trial Investigators: Trastuzumab in combination with chemotherapy versus chemotherapy alone for treatment of HER2-positive advanced gastric or gastro-oesophageal junction cancer (ToGA): A phase 3, open-label, randomised controlled trial. Lancet. 376:687–697. 2010. View Article : Google Scholar : PubMed/NCBI

6 

Roder JD, Böttcher K, Busch R, Wittekind C, Hermanek P and Siewert JR: German Gastric Cancer Study Group: Classification of regional lymph node metastasis from gastric carcinoma. Cancer. 82:621–631. 1998. View Article : Google Scholar : PubMed/NCBI

7 

Chen J, Cheong JH, Yun MJ, Kim J, Lim JS, Hyung WJ and Noh SH: Improvement in preoperative staging of gastric adenocarcinoma with positron emission tomography. Cancer. 103:2383–2390. 2005. View Article : Google Scholar : PubMed/NCBI

8 

O'Hayre M, Vázquez-Prado J, Kufareva I, Stawiski EW, Handel TM, Seshagiri S and Gutkind JS: The emerging mutational landscape of G proteins and G-protein-coupled receptors in cancer. Nat Rev Cancer. 13:412–424. 2013. View Article : Google Scholar : PubMed/NCBI

9 

Wang HJ, Zhou M, Jia L, Sun J, Shi HB, Liu SL and Wang ZZ: Identification of aberrant chromosomal regions in human breast cancer using gene expression data and related gene information. Med Sci Monit. 21:2557–2566. 2015. View Article : Google Scholar : PubMed/NCBI

10 

Lo FY, Chang JW, Chang IS, Chen YJ, Hsu HS, Huang SF, Tsai FY, Jiang SS, Kanteti R, Nandi S, et al: The database of chromosome imbalance regions and genes resided in lung cancer from Asian and Caucasian identified by array-comparative genomic hybridization. BMC Cancer. 12:2352012. View Article : Google Scholar : PubMed/NCBI

11 

Cannon-Albright LA, Teerlink CC, Farnham JM, Thomas AW, Zone JJ and Leachman SA: Linkage analysis of extended high-risk pedigrees replicates a cutaneous malignant melanoma predisposition locus on chromosome 9q21. J Invest Dermatol. 133:128–134. 2013. View Article : Google Scholar : PubMed/NCBI

12 

Koh Y, Park I, Sun CH, Lee S, Yun H, Park CK, Park SH, Park JK and Lee SH: Detection of a distinctive genomic signature in rhabdoid glioblastoma, a rare disease entity identified by whole exome sequencing and whole transcriptome sequencing. Transl Oncol. 8:279–287. 2015. View Article : Google Scholar : PubMed/NCBI

13 

Vaqué JP, Dorsam RT, Feng X, Iglesias-Bartolome R, Forsthoefel DJ, Chen Q, Debant A, Seeger MA, Ksander BR, Teramoto H, et al: A genome-wide RNAi screen reveals a Trio-regulated Rho GTPase circuitry transducing mitogenic signals initiated by G protein-coupled receptors. Mol Cell. 49:94–108. 2013. View Article : Google Scholar : PubMed/NCBI

14 

Van Raamsdonk CD, Griewank KG, Crosby MB, Garrido MC, Vemula S, Wiesner T, Obenauf AC, Wackernagel W, Green G, Bouvier N, et al: Mutations in GNA11 in uveal melanoma. N Engl J Med. 363:2191–2199. 2010. View Article : Google Scholar : PubMed/NCBI

15 

Lee CH, Park D, Wu D, Rhee SG and Simon MI: Members of the Gq α subunit gene family activate phospholipase C β isozymes. J Biol Chem. 267:16044–16047. 1992.PubMed/NCBI

16 

Rozengurt E: Mitogenic signaling pathways induced by G protein-coupled receptors. J Cell Physiol. 213:589–602. 2007. View Article : Google Scholar : PubMed/NCBI

17 

Cobb MH and Goldsmith EJ: How MAP kinases are regulated. J Biol Chem. 270:14843–14846. 1995. View Article : Google Scholar : PubMed/NCBI

18 

Karin M: The regulation of AP-1 activity by mitogen-activated protein kinases. J Biol Chem. 270:16483–16486. 1995. View Article : Google Scholar : PubMed/NCBI

19 

Dhawan P and Richmond A: A novel NF-kappa B-inducing kinase-MAPK signaling pathway up-regulates NF-kappa B activity in melanoma cells. J Biol Chem. 277:7920–7928. 2002. View Article : Google Scholar : PubMed/NCBI

20 

Kortylewski M, Heinrich PC, Kauffmann ME, Böhm M, MacKiewicz A and Behrmann I: Mitogen-activated protein kinases control p27/Kip1 expression and growth of human melanoma cells. Biochem J. 357:297–303. 2001. View Article : Google Scholar : PubMed/NCBI

21 

Roberts PJ and Der CJ: Targeting the Raf-MEK-ERK mitogen-activated protein kinase cascade for the treatment of cancer. Oncogene. 26:3291–3310. 2007. View Article : Google Scholar : PubMed/NCBI

22 

Weber JD, Raben DM, Phillips PJ and Baldassare JJ: Sustained activation of extracellular-signal-regulated kinase 1 (ERK1) is required for the continued expression of cyclin D1 in G1 phase. Biochem J. 326:61–68. 1997. View Article : Google Scholar : PubMed/NCBI

23 

Schwartz MA and Assoian RK: Integrins and cell proliferation: Regulation of cyclin-dependent kinases via cytoplasmic signaling pathways. J Cell Sci. 114:2553–2560. 2001.PubMed/NCBI

24 

Van Raamsdonk CD, Bezrookove V, Green G, Bauer J, Gaugler L, O'Brien JM, Simpson EM, Barsh GS and Bastian BC: Frequent somatic mutations of GNAQ in uveal melanoma and blue naevi. Nature. 457:599–602. 2009. View Article : Google Scholar : PubMed/NCBI

25 

Lane DP: Cancer. p53, guardian of the genome. Nature. 358:15–16. 1992. View Article : Google Scholar : PubMed/NCBI

26 

Liu J, Zhang C and Feng Z: Tumor suppressor p53 and its gain-of-function mutants in cancer. Acta Biochim Biophys Sin. 46:170–179. 2014. View Article : Google Scholar : PubMed/NCBI

27 

Riley T, Sontag E, Chen P and Levine A: Transcriptional control of human p53-regulated genes. Nat Rev Mol Cell Biol. 9:402–412. 2008. View Article : Google Scholar : PubMed/NCBI

28 

Mirza A, Wu Q, Wang L, McClanahan T, Bishop WR, Gheyas F, Ding W, Hutchins B, Hockenberry T, Kirschmeier P, et al: Global transcriptional program of p53 target genes during the process of apoptosis and cell cycle progression. Oncogene. 22:3645–3654. 2003. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Wang Y, Xiao H, Wu H, Yao C, He H, Wang C and Li W: G protein subunit α q regulates gastric cancer growth via the p53/p21 and MEK/ERK pathways. Oncol Rep 37: 1998-2006, 2017.
APA
Wang, Y., Xiao, H., Wu, H., Yao, C., He, H., Wang, C., & Li, W. (2017). G protein subunit α q regulates gastric cancer growth via the p53/p21 and MEK/ERK pathways. Oncology Reports, 37, 1998-2006. https://doi.org/10.3892/or.2017.5500
MLA
Wang, Y., Xiao, H., Wu, H., Yao, C., He, H., Wang, C., Li, W."G protein subunit α q regulates gastric cancer growth via the p53/p21 and MEK/ERK pathways". Oncology Reports 37.4 (2017): 1998-2006.
Chicago
Wang, Y., Xiao, H., Wu, H., Yao, C., He, H., Wang, C., Li, W."G protein subunit α q regulates gastric cancer growth via the p53/p21 and MEK/ERK pathways". Oncology Reports 37, no. 4 (2017): 1998-2006. https://doi.org/10.3892/or.2017.5500
Copy and paste a formatted citation
x
Spandidos Publications style
Wang Y, Xiao H, Wu H, Yao C, He H, Wang C and Li W: G protein subunit α q regulates gastric cancer growth via the p53/p21 and MEK/ERK pathways. Oncol Rep 37: 1998-2006, 2017.
APA
Wang, Y., Xiao, H., Wu, H., Yao, C., He, H., Wang, C., & Li, W. (2017). G protein subunit α q regulates gastric cancer growth via the p53/p21 and MEK/ERK pathways. Oncology Reports, 37, 1998-2006. https://doi.org/10.3892/or.2017.5500
MLA
Wang, Y., Xiao, H., Wu, H., Yao, C., He, H., Wang, C., Li, W."G protein subunit α q regulates gastric cancer growth via the p53/p21 and MEK/ERK pathways". Oncology Reports 37.4 (2017): 1998-2006.
Chicago
Wang, Y., Xiao, H., Wu, H., Yao, C., He, H., Wang, C., Li, W."G protein subunit α q regulates gastric cancer growth via the p53/p21 and MEK/ERK pathways". Oncology Reports 37, no. 4 (2017): 1998-2006. https://doi.org/10.3892/or.2017.5500
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