Open Access

Circulating microRNA-194 regulates human melanoma cells via PI3K/AKT/FoxO3a and p53/p21 signaling pathway

  • Authors:
    • Ming Bai
    • Mingzi Zhang
    • Fei Long
    • Nanze Yu
    • Ang Zeng
    • Ru Zhao
  • View Affiliations

  • Published online on: March 30, 2017     https://doi.org/10.3892/or.2017.5537
  • Pages: 2702-2710
  • Copyright: © Bai et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

In the present study, we analyzed the role of microRNA-194 circulating regulated human melanoma cell growth. We found that microRNA-194 expression was markedly suppressed in human melanoma patients, compared with negative control group. Next, disease-free survival (DFS) and overall survival (OS) of high expression in human melanoma patients was higher than those of low expression in human melanoma patients. MicroRNA-194 overexpression inhibited cell proliferation, induced apoptosis, increased caspase-3/-9 activities and promoted Bax/Bcl-2 of human melanoma cells. Furthermore, microRNA-194 overexpression also suppressed PI3K/AKT/FoxO3a signaling pathway and induced p53/p21 signaling pathway. PI3K inhibitor, suppressed PI3K, phosphorylation-AKT, FoxO3a protein expression and increased the effects of microRNA-194 overexpression on cell growth, apoptosis, caspase-3/-9 activities and Bax/Bcl-2 protein expression of human melanoma cells through the induction of p53/p21 signaling pathway. Taken together, these data indicate that circulating microRNA-194 regulated human melanoma cells via PI3K/AKT/FoxO3a and p53/p21 signaling pathway.
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May-2017
Volume 37 Issue 5

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Bai M, Zhang M, Long F, Yu N, Zeng A and Zhao R: Circulating microRNA-194 regulates human melanoma cells via PI3K/AKT/FoxO3a and p53/p21 signaling pathway. Oncol Rep 37: 2702-2710, 2017
APA
Bai, M., Zhang, M., Long, F., Yu, N., Zeng, A., & Zhao, R. (2017). Circulating microRNA-194 regulates human melanoma cells via PI3K/AKT/FoxO3a and p53/p21 signaling pathway. Oncology Reports, 37, 2702-2710. https://doi.org/10.3892/or.2017.5537
MLA
Bai, M., Zhang, M., Long, F., Yu, N., Zeng, A., Zhao, R."Circulating microRNA-194 regulates human melanoma cells via PI3K/AKT/FoxO3a and p53/p21 signaling pathway". Oncology Reports 37.5 (2017): 2702-2710.
Chicago
Bai, M., Zhang, M., Long, F., Yu, N., Zeng, A., Zhao, R."Circulating microRNA-194 regulates human melanoma cells via PI3K/AKT/FoxO3a and p53/p21 signaling pathway". Oncology Reports 37, no. 5 (2017): 2702-2710. https://doi.org/10.3892/or.2017.5537