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Article

SPAG6 regulates cell apoptosis through the TRAIL signal pathway in myelodysplastic syndromes

  • Authors:
    • Xinxin Li
    • Bihui Yang
    • Li Wang
    • Liping Chen
    • Xiaohua Luo
    • Lin Liu
  • View Affiliations / Copyright

    Affiliations: Department of Hematology, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, P.R. China
  • Pages: 2839-2846
    |
    Published online on: March 31, 2017
       https://doi.org/10.3892/or.2017.5540
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Abstract

Myelodysplastic syndromes (MDSs) are a group of malignant clone hematopoietic stem-cell diseases, and the evolution and progression of MDS depend on the abnormal apoptosis of bone marrow cells. Our previous studies have indicated that sperm-associated antigen 6 (SPAG6), located in the uniparental disomy regions of myeloid cells, is overexpressed in patients with MDS as compared to controls, and SPAG6 can inhibit apoptosis of SKM-1. However, the concrete mechanism is still unclear. In the present study, it was found that the TNF-related apoptosis-inducing ligand (TRAIL)signal pathway was activated when the expression of SPAG6 was inhibited by SPAG6-shRNA lentivirus in SKM-1 cells. Additionally, the results of flow cytometry, Cell Counting Kit-8 assay and western blot analysis implied that the TRAIL signal pathway could be inhibited by a high expression of SPAG6. However, SPAG6 cannot influence the expression of TRAIL death receptors, except for FADD. Additionally the interaction between FADD and TRAIL death receptors also increased in SKM-1 cells infected with SPAG6-shRNA lentivirus. Thus, our study demonstrates that SPAG6 may regulate apoptosis in SKM-1 through the TRAIL signal pathway, indicating that SPAG6 could be a potential therapeutic target.
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Copy and paste a formatted citation
Spandidos Publications style
Li X, Yang B, Wang L, Chen L, Luo X and Liu L: SPAG6 regulates cell apoptosis through the TRAIL signal pathway in myelodysplastic syndromes. Oncol Rep 37: 2839-2846, 2017.
APA
Li, X., Yang, B., Wang, L., Chen, L., Luo, X., & Liu, L. (2017). SPAG6 regulates cell apoptosis through the TRAIL signal pathway in myelodysplastic syndromes. Oncology Reports, 37, 2839-2846. https://doi.org/10.3892/or.2017.5540
MLA
Li, X., Yang, B., Wang, L., Chen, L., Luo, X., Liu, L."SPAG6 regulates cell apoptosis through the TRAIL signal pathway in myelodysplastic syndromes". Oncology Reports 37.5 (2017): 2839-2846.
Chicago
Li, X., Yang, B., Wang, L., Chen, L., Luo, X., Liu, L."SPAG6 regulates cell apoptosis through the TRAIL signal pathway in myelodysplastic syndromes". Oncology Reports 37, no. 5 (2017): 2839-2846. https://doi.org/10.3892/or.2017.5540
Copy and paste a formatted citation
x
Spandidos Publications style
Li X, Yang B, Wang L, Chen L, Luo X and Liu L: SPAG6 regulates cell apoptosis through the TRAIL signal pathway in myelodysplastic syndromes. Oncol Rep 37: 2839-2846, 2017.
APA
Li, X., Yang, B., Wang, L., Chen, L., Luo, X., & Liu, L. (2017). SPAG6 regulates cell apoptosis through the TRAIL signal pathway in myelodysplastic syndromes. Oncology Reports, 37, 2839-2846. https://doi.org/10.3892/or.2017.5540
MLA
Li, X., Yang, B., Wang, L., Chen, L., Luo, X., Liu, L."SPAG6 regulates cell apoptosis through the TRAIL signal pathway in myelodysplastic syndromes". Oncology Reports 37.5 (2017): 2839-2846.
Chicago
Li, X., Yang, B., Wang, L., Chen, L., Luo, X., Liu, L."SPAG6 regulates cell apoptosis through the TRAIL signal pathway in myelodysplastic syndromes". Oncology Reports 37, no. 5 (2017): 2839-2846. https://doi.org/10.3892/or.2017.5540
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