CUTL1 induces epithelial-mesenchymal transition in non-small cell lung cancer

Wang,Junfeng; Wang,Yanbo; Sun,Dawei; Ren,Fenghai; Pang,Sainan; Xu,Shidong

doi:10.3892/or.2017.5571

CUTL1 induces epithelial-mesenchymal transition in non-small cell lung cancer

Authors:
- Junfeng Wang
- Yanbo Wang
- Dawei Sun
- Fenghai Ren
- Sainan Pang
- Shidong Xu
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Affiliations: Department of Thoracic Surgery, Harbin Medical University Cancer Hospital, Harbin, Heilongjiang 150081, P.R. China
Published online on: April 11, 2017 https://doi.org/10.3892/or.2017.5571
Pages: 3068-3074

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Abstract

The homeobox transcription factor CUTL1 has been associated with cellular proliferation and cell cycle progression, and CUTL1 functions as an oncogene. The aim of the present study was to investigate whether CUTL1 participates in epithelial-mesenchymal transition (EMT). The expression levels of CUTL1, E-cadherin, N-cadherin and Snail were determined by immunohistochemistry assay, immunofluorescence assay or real-time quantitative reverse transcription PCR. Their roles in non-small cell lung cancer (NSCLC) were assessed by functional analyses. Protein expression was detected by western blot analysis. The CUTL1 expression levels are higher in non-small cell lung cancer (NSCLC) tissues. High CUTL1 expression in NSCLC is associated with the mesenchymal-like phenotype. Mechanistically, CUTL1 upregulates transforming growth factor β receptor I (TβR-I) expression, and the TβR-I inhibitor SB431542 abolishes EMT elicited by ectopic CUTL1 expression. Transforming growth factor β (TGF-β) signaling is essential for CUTL1-induced EMT in NSCLC cells. CUTL1 is downstream of TGF-β signaling and CUTL1 is involved in the expression of the TβR-I. This study indicates that CUTL1 may be a potential target for anti-lung cancer therapy.

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