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Article Open Access

GINS2 is a novel prognostic biomarker and promotes tumor progression in early-stage cervical cancer

Corrigendum in: /10.3892/or.2021.8016
  • Authors:
    • Fei Ouyang
    • Junling Liu
    • Meng Xia
    • Chuyong Lin
    • Xianqiu Wu
    • Liping Ye
    • Libing Song
    • Jun Li
    • Jing Wang
    • Peng Guo
    • Mian He
  • View Affiliations / Copyright

    Affiliations: Department of Gynecology and Obstetrics, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, P.R. China, State Key Laboratory of Oncology in Southern China, Collaborative Innovation Center for Cancer Medicine, Department of Medical Oncology, Sun Yat-sen University Cancer Center, Guangzhou, P.R. China, State Key Laboratory of Oncology in Southern China, Department of Experimental Research, Collaborative Innovation Center for Cancer Medicine, Sun Yat-sen University Cancer Center, Guangzhou, P.R. China, Department of Biochemistry, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, P.R. China
    Copyright: © Ouyang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2652-2662
    |
    Published online on: April 11, 2017
       https://doi.org/10.3892/or.2017.5573
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Abstract

GINS complex subunit 2 (GINS2), a member of the GINS complex, is involved in DNA replication. GINS2 is upregulated in a variety of aggressive tumors. However, its role in cervical cancer carcinogenesis remains to be elucidated. We investigated the clinical significance of GINS2 in patients with early-stage cervical cancer and its biological functions in cervical cancer progression. GINS2 expression was analyzed in cervical cancer cell lines and in 8 matched cervical cancer samples at the mRNA and protein levels using real-time PCR and western blotting, respectively. GINS2 protein expression in 155 paraffin-embedded cervical cancer specimens was validated using immunohistochemistry. Statistical analysis was used to evaluate its clinicopathological significance. Short hairpin RNA interference, anchorage-independent growth ability, colony formation assay, wound healing ability, Transwell assays and western blotting were used to determine the effects of GINS2 on the aggressive phenotype of cervical cancer cells. There was obvious upregulation of GINS2 in the cervical cancer cell lines and tumor specimens compared to that in the normal cervical tissues. Significant correlations were identified between GINS2 expression and squamous cell carcinoma antigen (SCC-Ag; P<0.001), deep stromal invasion (P=0.021), vital status (P<0.001), recurrence (P<0.001) and pelvic lymph node metastasis (PLNM; P<0.001). Moreover, patients with higher GINS2 expression had shorter overall survival (OS) compared to patients with low GINS2 expression. Multivariate analysis revealed that GINS2 may serve as an independent risk factor of poor prognosis in early-stage cervical cancer. In addition, GINS2 downregulation markedly suppressed cell proliferation and tumorigenic ability, as well as cell migration and invasion. Our findings suggest that GINS2 is a novel indicator of PLNM and a valuable prognostic biomarker in early-stage cervical cancer, and subsequently is a valuable molecular target for cervical cancer diagnosis and treatment.
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Copy and paste a formatted citation
Spandidos Publications style
Ouyang F, Liu J, Xia M, Lin C, Wu X, Ye L, Song L, Li J, Wang J, Guo P, Guo P, et al: GINS2 is a novel prognostic biomarker and promotes tumor progression in early-stage cervical cancer Corrigendum in /10.3892/or.2021.8016. Oncol Rep 37: 2652-2662, 2017.
APA
Ouyang, F., Liu, J., Xia, M., Lin, C., Wu, X., Ye, L. ... He, M. (2017). GINS2 is a novel prognostic biomarker and promotes tumor progression in early-stage cervical cancer Corrigendum in /10.3892/or.2021.8016. Oncology Reports, 37, 2652-2662. https://doi.org/10.3892/or.2017.5573
MLA
Ouyang, F., Liu, J., Xia, M., Lin, C., Wu, X., Ye, L., Song, L., Li, J., Wang, J., Guo, P., He, M."GINS2 is a novel prognostic biomarker and promotes tumor progression in early-stage cervical cancer Corrigendum in /10.3892/or.2021.8016". Oncology Reports 37.5 (2017): 2652-2662.
Chicago
Ouyang, F., Liu, J., Xia, M., Lin, C., Wu, X., Ye, L., Song, L., Li, J., Wang, J., Guo, P., He, M."GINS2 is a novel prognostic biomarker and promotes tumor progression in early-stage cervical cancer Corrigendum in /10.3892/or.2021.8016". Oncology Reports 37, no. 5 (2017): 2652-2662. https://doi.org/10.3892/or.2017.5573
Copy and paste a formatted citation
x
Spandidos Publications style
Ouyang F, Liu J, Xia M, Lin C, Wu X, Ye L, Song L, Li J, Wang J, Guo P, Guo P, et al: GINS2 is a novel prognostic biomarker and promotes tumor progression in early-stage cervical cancer Corrigendum in /10.3892/or.2021.8016. Oncol Rep 37: 2652-2662, 2017.
APA
Ouyang, F., Liu, J., Xia, M., Lin, C., Wu, X., Ye, L. ... He, M. (2017). GINS2 is a novel prognostic biomarker and promotes tumor progression in early-stage cervical cancer Corrigendum in /10.3892/or.2021.8016. Oncology Reports, 37, 2652-2662. https://doi.org/10.3892/or.2017.5573
MLA
Ouyang, F., Liu, J., Xia, M., Lin, C., Wu, X., Ye, L., Song, L., Li, J., Wang, J., Guo, P., He, M."GINS2 is a novel prognostic biomarker and promotes tumor progression in early-stage cervical cancer Corrigendum in /10.3892/or.2021.8016". Oncology Reports 37.5 (2017): 2652-2662.
Chicago
Ouyang, F., Liu, J., Xia, M., Lin, C., Wu, X., Ye, L., Song, L., Li, J., Wang, J., Guo, P., He, M."GINS2 is a novel prognostic biomarker and promotes tumor progression in early-stage cervical cancer Corrigendum in /10.3892/or.2021.8016". Oncology Reports 37, no. 5 (2017): 2652-2662. https://doi.org/10.3892/or.2017.5573
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