MicroRNA-26a inhibits proliferation and metastasis of human hepatocellular carcinoma by regulating DNMT3B-MEG3 axis

  • Authors:
    • Yarui Li
    • Mudan Ren
    • Yan Zhao
    • Xinlan Lu
    • Mengyao Wang
    • Junbi Hu
    • Guifang Lu
    • Shuixiang He
  • View Affiliations

  • Published online on: April 19, 2017     https://doi.org/10.3892/or.2017.5579
  • Pages: 3527-3535
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Abstract

miR-26a is known to play an important oncosuppressive role in HCC. However, its regulatory role and relationship with other non-coding RNAs is less clear. In the present study, we report that the expression levels of miR-26a and long non-coding RNA (lncRNA) maternally expressed gene 3 (MEG3) were frequently downregulated in HCC tissues compared to matched non-malignant tissues. In addition, the expression levels of miR-26a and MEG3 were negatively correlated with the tumor sizes and TNM clinical stage in HCC patients. Overexpression of miR-26a significantly reduced the capacity of proliferation, invasion and migration of HCC cells. Moreover, we demonstrated that DNA methyltransferase 3b (DNMT3B) was a direct target gene of miR-26a. Overexpression of miR-26a suppressed the expression level of DNMT3B. Inhibited expression of DNMT3B showed similar tumor suppressive effects induced by miR-26a upregulation, and resulted in the upregulation of MEG3. Furthermore, we found that the expression levels of DNMT3B were upregulated in the HCC tissues compared with non-malignant tissues, and it was inversely correlated with miR-26a and MEG3 in HCC tissues. Thus, these results provided a plausible link between the observed reduction of miR-26a and MEG3 in HCCs. Together, the present study added miR-26a/DNMT3B/MEG3 axis to the complex mechanisms of HCC development.
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June-2017
Volume 37 Issue 6

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Li Y, Ren M, Zhao Y, Lu X, Wang M, Hu J, Lu G and He S: MicroRNA-26a inhibits proliferation and metastasis of human hepatocellular carcinoma by regulating DNMT3B-MEG3 axis. Oncol Rep 37: 3527-3535, 2017
APA
Li, Y., Ren, M., Zhao, Y., Lu, X., Wang, M., Hu, J. ... He, S. (2017). MicroRNA-26a inhibits proliferation and metastasis of human hepatocellular carcinoma by regulating DNMT3B-MEG3 axis. Oncology Reports, 37, 3527-3535. https://doi.org/10.3892/or.2017.5579
MLA
Li, Y., Ren, M., Zhao, Y., Lu, X., Wang, M., Hu, J., Lu, G., He, S."MicroRNA-26a inhibits proliferation and metastasis of human hepatocellular carcinoma by regulating DNMT3B-MEG3 axis". Oncology Reports 37.6 (2017): 3527-3535.
Chicago
Li, Y., Ren, M., Zhao, Y., Lu, X., Wang, M., Hu, J., Lu, G., He, S."MicroRNA-26a inhibits proliferation and metastasis of human hepatocellular carcinoma by regulating DNMT3B-MEG3 axis". Oncology Reports 37, no. 6 (2017): 3527-3535. https://doi.org/10.3892/or.2017.5579