Effective cytotoxic activity of OSW-1 on colon cancer by inducing apoptosis in vitro and in vivo

Zhang,Yanhong; Fang,Fengqi; Fan,Kai; Zhang,Yanli; Zhang,Jie; Guo,Huishu; Yu,Peiyao; Ma,Jianmei

doi:10.3892/or.2017.5582

Effective cytotoxic activity of OSW-1 on colon cancer by inducing apoptosis in vitro and in vivo

Authors:
- Yanhong Zhang
- Fengqi Fang
- Kai Fan
- Yanli Zhang
- Jie Zhang
- Huishu Guo
- Peiyao Yu
- Jianmei Ma
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Affiliations: Graduate School of Dalian Medical University, Dalian, Liaoning 116044, P.R. China, The First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning 116011, P.R. China, Department of Anatomy, Dalian Medical University, Dalian, Liaoning 116044, P.R. China, Centralab, The First Afﬁliated Hospital of Dalian Medical University, Dalian, Liaoning 116044, P.R. China
Published online on: April 19, 2017 https://doi.org/10.3892/or.2017.5582
Pages: 3509-3519

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Abstract

As a natural compound, Ornithogalum caudatum Ait is primarily used as an anti-inflammatory and antitumor agent in Chinese folk medicine. In 1992, OSW-1 was isolated from this compound, which is a new member of cholestane saponin family. In numerous recent studies, OSW-1 has been shown to have powerful cytotoxic anticancer effects against various malignant cells. However, the therapeutic efficacy of OSW-1 on colon cancer and the underlying mechanism are not understood. To explore the mechanism underlying OSW-1 in antitumor therapy, a therapeutic function analysis of OSW-1 on colon cancer was performed in vitro and in vivo. It was shown that with low toxicity on normal colonic cells, OSW-1 suppresses colon cancer cells in vitro and this inhibition was via the intrinsic apoptotic pathway, which increased cellular calcium, changed mitochondrial membrane potential, disrupted mitochondrial morphology, and led to the release of cytochrome c and the activation of caspase-3. Furthermore, in a nude mouse model, OSW-1 had a powerful effect on suppressing colon tumor proliferation without significant side effects through the apoptosis pathway. Taken together, these results demonstrate that OSW-1 is a potential drug for colon cancer treatment.

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