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Article

Migration of oral squamous cell carcinoma cells are induced by HGF/c-Met signalling via lamellipodia and filopodia formation

  • Authors:
    • Hiroki Yasui
    • Yuichi Ohnishi
    • Masahiro Nakajima
    • Masami Nozaki
  • View Affiliations / Copyright

    Affiliations: Department of Cell Biology, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka 565-0871, Japan, Second Department of Oral and Maxillofacial Surgery, Osaka Dental University, Hirakata, Osaka 573-1121, Japan
  • Pages: 3674-3680
    |
    Published online on: April 19, 2017
       https://doi.org/10.3892/or.2017.5587
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Abstract

The activation of receptor tyrosine kinases (RTKs) results in cellular effects including cell proliferation, survival, migration and invasion; RTKs also play an important role in tumourigenesis. It has been reported that EGFR signalling controls the migration of oral squamous cell carcinoma (OSCC) SAS and HSC3 cells but not of HSC4 cells, although the proliferation of HSC4 cells is regulated by EGF/EGFR. In the present study, we investigated the roles of EGFR and the c-Met signalling pathway in cell migration via filopodia and lamellipodia formation, which may be prerequisites for migration. To explore the role of c-Met in cell migration, we inhibited c-Met RTK activity using the c-Met inhibitor SU11274 and activated c-Met using hepatocyte growth factor (HGF) in three OSCC cell lines HSC4, SAS and Ca9-22 and investigated migration potency using a wound healing assay. We showed that inhibition of c-Met significantly suppressed, and activation of c-Met significantly promoted, the migration of OSCC cells. Additionally, the migration of SAS and Ca9-22 cells was inhibited by the EGFR inhibitors AG1478 and cetuximab and promoted by EGF treatment. Moreover, migration potency was correlated with lamellipodia formation. Furthermore, western blot analyses demonstrated that SU11274 decreased and HGF increased lamellipodin protein levels as well as phosphorylated c-Met levels. Collectively, we demonstrated that c-Met signalling induced lamellipodia formation by upregulating lamellipodin, thereby promoting the migration of OSCC cells.
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Copy and paste a formatted citation
Spandidos Publications style
Yasui H, Ohnishi Y, Nakajima M and Nozaki M: Migration of oral squamous cell carcinoma cells are induced by HGF/c-Met signalling via lamellipodia and filopodia formation. Oncol Rep 37: 3674-3680, 2017.
APA
Yasui, H., Ohnishi, Y., Nakajima, M., & Nozaki, M. (2017). Migration of oral squamous cell carcinoma cells are induced by HGF/c-Met signalling via lamellipodia and filopodia formation. Oncology Reports, 37, 3674-3680. https://doi.org/10.3892/or.2017.5587
MLA
Yasui, H., Ohnishi, Y., Nakajima, M., Nozaki, M."Migration of oral squamous cell carcinoma cells are induced by HGF/c-Met signalling via lamellipodia and filopodia formation". Oncology Reports 37.6 (2017): 3674-3680.
Chicago
Yasui, H., Ohnishi, Y., Nakajima, M., Nozaki, M."Migration of oral squamous cell carcinoma cells are induced by HGF/c-Met signalling via lamellipodia and filopodia formation". Oncology Reports 37, no. 6 (2017): 3674-3680. https://doi.org/10.3892/or.2017.5587
Copy and paste a formatted citation
x
Spandidos Publications style
Yasui H, Ohnishi Y, Nakajima M and Nozaki M: Migration of oral squamous cell carcinoma cells are induced by HGF/c-Met signalling via lamellipodia and filopodia formation. Oncol Rep 37: 3674-3680, 2017.
APA
Yasui, H., Ohnishi, Y., Nakajima, M., & Nozaki, M. (2017). Migration of oral squamous cell carcinoma cells are induced by HGF/c-Met signalling via lamellipodia and filopodia formation. Oncology Reports, 37, 3674-3680. https://doi.org/10.3892/or.2017.5587
MLA
Yasui, H., Ohnishi, Y., Nakajima, M., Nozaki, M."Migration of oral squamous cell carcinoma cells are induced by HGF/c-Met signalling via lamellipodia and filopodia formation". Oncology Reports 37.6 (2017): 3674-3680.
Chicago
Yasui, H., Ohnishi, Y., Nakajima, M., Nozaki, M."Migration of oral squamous cell carcinoma cells are induced by HGF/c-Met signalling via lamellipodia and filopodia formation". Oncology Reports 37, no. 6 (2017): 3674-3680. https://doi.org/10.3892/or.2017.5587
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