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International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

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International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

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Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

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Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

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Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

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Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

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Article

CRISPR/Cas9-mediated genome engineering of CXCR4 decreases the malignancy of hepatocellular carcinoma cells in vitro and in vivo

  • Authors:
    • Xiaoli Wang
    • Wenmei Zhang
    • Yan Ding
    • Xingrong Guo
    • Yahong Yuan
    • Dongsheng Li
  • View Affiliations / Copyright

    Affiliations: Hubei Key Laboratory of Embryonic Stem Cell Research, Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei 442000, P.R. China
  • Pages: 3565-3571
    |
    Published online on: April 25, 2017
       https://doi.org/10.3892/or.2017.5601
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Abstract

CXC chemokine receptor 4 (CXCR4) is associated with poor clinical outcomes and decreased survival in hepatocellular carcinoma (HCC). In the present study, we targeted CXCR4 by CRISPR/Cas9 in HepG2 cells and observed the effects both in vitro and in vivo. The results indicated that after targeting CXCR4 the expression of CXCR4 was significantly decreased and the cell proliferation was inhibited. Clonogenicity and scratch cell migration assays indicated that specific downregulation of CXCR4 inhibited cell migration. This disruption of CXCR4 led to less invasiveness, the genes related to epithelial-mesenchymal transition (EMT) and cell self-renewal were also affected. Moreover, sensitivity to the anticancer drug cisplatin was significantly increased in vitro by the downregulation of CXCR4. The results of the in vivo study showed that the growth volumes were significantly smaller in neoplasms derived from CXCR4-downregulated HepG2 cells compared to those derived from wild-type cells. These results showed that targeting CXCR4 by CRISPR/Cas9 could inhibit proliferation, migration and invasion, reversed EMT, increased chemosensitivity and decrease the malignancy of HCC in vitro and in vivo.
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Copy and paste a formatted citation
Spandidos Publications style
Wang X, Zhang W, Ding Y, Guo X, Yuan Y and Li D: CRISPR/Cas9-mediated genome engineering of CXCR4 decreases the malignancy of hepatocellular carcinoma cells in vitro and in vivo. Oncol Rep 37: 3565-3571, 2017.
APA
Wang, X., Zhang, W., Ding, Y., Guo, X., Yuan, Y., & Li, D. (2017). CRISPR/Cas9-mediated genome engineering of CXCR4 decreases the malignancy of hepatocellular carcinoma cells in vitro and in vivo. Oncology Reports, 37, 3565-3571. https://doi.org/10.3892/or.2017.5601
MLA
Wang, X., Zhang, W., Ding, Y., Guo, X., Yuan, Y., Li, D."CRISPR/Cas9-mediated genome engineering of CXCR4 decreases the malignancy of hepatocellular carcinoma cells in vitro and in vivo". Oncology Reports 37.6 (2017): 3565-3571.
Chicago
Wang, X., Zhang, W., Ding, Y., Guo, X., Yuan, Y., Li, D."CRISPR/Cas9-mediated genome engineering of CXCR4 decreases the malignancy of hepatocellular carcinoma cells in vitro and in vivo". Oncology Reports 37, no. 6 (2017): 3565-3571. https://doi.org/10.3892/or.2017.5601
Copy and paste a formatted citation
x
Spandidos Publications style
Wang X, Zhang W, Ding Y, Guo X, Yuan Y and Li D: CRISPR/Cas9-mediated genome engineering of CXCR4 decreases the malignancy of hepatocellular carcinoma cells in vitro and in vivo. Oncol Rep 37: 3565-3571, 2017.
APA
Wang, X., Zhang, W., Ding, Y., Guo, X., Yuan, Y., & Li, D. (2017). CRISPR/Cas9-mediated genome engineering of CXCR4 decreases the malignancy of hepatocellular carcinoma cells in vitro and in vivo. Oncology Reports, 37, 3565-3571. https://doi.org/10.3892/or.2017.5601
MLA
Wang, X., Zhang, W., Ding, Y., Guo, X., Yuan, Y., Li, D."CRISPR/Cas9-mediated genome engineering of CXCR4 decreases the malignancy of hepatocellular carcinoma cells in vitro and in vivo". Oncology Reports 37.6 (2017): 3565-3571.
Chicago
Wang, X., Zhang, W., Ding, Y., Guo, X., Yuan, Y., Li, D."CRISPR/Cas9-mediated genome engineering of CXCR4 decreases the malignancy of hepatocellular carcinoma cells in vitro and in vivo". Oncology Reports 37, no. 6 (2017): 3565-3571. https://doi.org/10.3892/or.2017.5601
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