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Article

Sulforaphane sensitizes human cholangiocarcinoma to cisplatin via the downregulation of anti-apoptotic proteins

Corrigendum in: /10.3892/or.2023.8646
Retraction in: /10.3892/or.2024.8728
  • Authors:
    • Rokas Račkauskas
    • Dachen Zhou
    • Simonas Ūselis
    • Kęstutis Strupas
    • Ingrid Herr
    • Peter Schemmer
  • View Affiliations / Copyright

    Affiliations: Department of General, Visceral and Transplant Surgery, University Hospital Heidelberg, D-69120 Heidelberg, Germany, Centre of Abdominal Surgery, Vilnius University, L-08661 Vilnius, Lithuania
  • Pages: 3660-3666
    |
    Published online on: May 3, 2017
       https://doi.org/10.3892/or.2017.5622
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Abstract

Cholangiocarcinoma (CCC) is an aggressive malignancy with poor therapeutic options and pronounced chemotherapy resistance. The bioactive broccoli substance, sulforaphane (SFN), is a promising new therapeutic option since it has been found to induce therapeutic effects in both experimental and epidemiological studies in various tumor entities. Thus, the present study was designed to assess the effect of SFN on cisplatin sensitivity in CCC. Human HuCCT-1 and TFK-1 cells, representing intrahepatic and extrahepatic CCC, respectively, were treated with cisplatin and SFN. Viability, the platinated DNA content, and apoptosis were assessed using both MTT assay and flow cytometry, while western blotting was used to analyze the expression of proteins involved in apoptosis and DNA damage. Whereas cisplatin was largely ineffective, SFN only therapy significantly decreased the viability of both CCC cell lines. The combination of SFN with cisplatin increased cisplatin cytotoxicity, which was particularly pronounced relatively early at 36 h after treatment. Apoptosis, which was reflected by the cleavage of caspase-3 and PARP, was significantly enhanced. Notably, only cisplatin was found to induce the expression of proteins involved in the DNA damage response; however, the presence of SFN appeared to enable otherwise cisplatin-resistant cells to undergo apoptosis. Due to the fact that SFN did not enhance the DNA platination levels upon cisplatin treatment, SFN may have exerted its activity via the inhibition of the anti-apoptotic proteins Bcl-2 and XIAP, as we observed. Data presented in the present study clearly demonstrated that SFN significantly decreased the drug resistance to cisplatin in human CCC. This highlights dietary co-treatment as a viable new treatment option for CCC.
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Copy and paste a formatted citation
Spandidos Publications style
Račkauskas R, Zhou D, Ūselis S, Strupas K, Herr I and Schemmer P: Sulforaphane sensitizes human cholangiocarcinoma to cisplatin via the downregulation of anti-apoptotic proteins Corrigendum in /10.3892/or.2023.8646 Retraction in /10.3892/or.2024.8728. Oncol Rep 37: 3660-3666, 2017.
APA
Račkauskas, R., Zhou, D., Ūselis, S., Strupas, K., Herr, I., & Schemmer, P. (2017). Sulforaphane sensitizes human cholangiocarcinoma to cisplatin via the downregulation of anti-apoptotic proteins Corrigendum in /10.3892/or.2023.8646 Retraction in /10.3892/or.2024.8728. Oncology Reports, 37, 3660-3666. https://doi.org/10.3892/or.2017.5622
MLA
Račkauskas, R., Zhou, D., Ūselis, S., Strupas, K., Herr, I., Schemmer, P."Sulforaphane sensitizes human cholangiocarcinoma to cisplatin via the downregulation of anti-apoptotic proteins Corrigendum in /10.3892/or.2023.8646 Retraction in /10.3892/or.2024.8728". Oncology Reports 37.6 (2017): 3660-3666.
Chicago
Račkauskas, R., Zhou, D., Ūselis, S., Strupas, K., Herr, I., Schemmer, P."Sulforaphane sensitizes human cholangiocarcinoma to cisplatin via the downregulation of anti-apoptotic proteins Corrigendum in /10.3892/or.2023.8646 Retraction in /10.3892/or.2024.8728". Oncology Reports 37, no. 6 (2017): 3660-3666. https://doi.org/10.3892/or.2017.5622
Copy and paste a formatted citation
x
Spandidos Publications style
Račkauskas R, Zhou D, Ūselis S, Strupas K, Herr I and Schemmer P: Sulforaphane sensitizes human cholangiocarcinoma to cisplatin via the downregulation of anti-apoptotic proteins Corrigendum in /10.3892/or.2023.8646 Retraction in /10.3892/or.2024.8728. Oncol Rep 37: 3660-3666, 2017.
APA
Račkauskas, R., Zhou, D., Ūselis, S., Strupas, K., Herr, I., & Schemmer, P. (2017). Sulforaphane sensitizes human cholangiocarcinoma to cisplatin via the downregulation of anti-apoptotic proteins Corrigendum in /10.3892/or.2023.8646 Retraction in /10.3892/or.2024.8728. Oncology Reports, 37, 3660-3666. https://doi.org/10.3892/or.2017.5622
MLA
Račkauskas, R., Zhou, D., Ūselis, S., Strupas, K., Herr, I., Schemmer, P."Sulforaphane sensitizes human cholangiocarcinoma to cisplatin via the downregulation of anti-apoptotic proteins Corrigendum in /10.3892/or.2023.8646 Retraction in /10.3892/or.2024.8728". Oncology Reports 37.6 (2017): 3660-3666.
Chicago
Račkauskas, R., Zhou, D., Ūselis, S., Strupas, K., Herr, I., Schemmer, P."Sulforaphane sensitizes human cholangiocarcinoma to cisplatin via the downregulation of anti-apoptotic proteins Corrigendum in /10.3892/or.2023.8646 Retraction in /10.3892/or.2024.8728". Oncology Reports 37, no. 6 (2017): 3660-3666. https://doi.org/10.3892/or.2017.5622
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