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Article Open Access

Improved xenograft efficiency of esophageal adenocarcinoma cell lines through in vivo selection

  • Authors:
    • Elodie Melsens
    • Elly De Vlieghere
    • Benedicte Descamps
    • Christian Vanhove
    • Olivier De Wever
    • Wim Ceelen
    • Piet Pattyn
  • View Affiliations / Copyright

    Affiliations: Laboratory of Experimental Surgery, Department of Surgery, Ghent University Hospital, Ghent, Belgium, Laboratory of Experimental Cancer Research, Department of Radiation Oncology and Experimental Cancer Research, Ghent University, Ghent, Belgium, Infinity (iMinds-IBiTech-MEDISIP), Department of Electronics and Information Systems, Ghent University, Ghent, Belgium
    Copyright: © Melsens et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 71-81
    |
    Published online on: May 15, 2017
       https://doi.org/10.3892/or.2017.5640
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Abstract

The present study aimed to investigate the orthotopic growth potential of two generally available esophageal adenocarcinoma cell lines, OE33 and OACM5 1.C, and a third in vivo selected subpopulation, OACM5 1.C SC1. One group of mice was subcutaneously injected in the hind legs. Tumor growth was measured with calipers. Another group was injected orthotopically in the distal esophageal wall through median laparotomy. Tumor development was evaluated macroscopically and confirmed microscopically. A subset of mice was evaluated with magnetic resonance imaging (MRI) to follow tumor progression. Additionally, functional cell line characteristics were evaluated in vitro (clonogenic, collagen invasion and sphere formation assays, and protein analysis of cell-cell adhesion and cytoskeletal proteins) to better understand xenograft behavior. OE33 cells were shown to be epithelial‑like, whereas OACM5 1.C and OACM5 1.C SC1 were more mesenchymal-like. The three cell lines were non‑invasive into native type I collagen gels. In vivo, OE33 cells led to 63.6 and 100% tumor nodules after orthotopic (n=12) and subcutaneous (n=8) injection, respectively. Adversely, OACM5 1.C cells did not lead to tumor formation after orthotopic injection (n=6) and only 50% of subcutaneous injections led to tumor nodules (n=8). However, the newly established cell line OACM5 1.C SC1 resulted in 33% tumor formation when orthotopically injected (n=6) and in 100% tumors when injected subcutaneously (n=8). The higher xenograft rate of OACM5 1.C SC1 (P<0.05) corresponded with a higher clonogenic potential compared to its parental cell line (P<0.0001). All models showed local tumor growth without metastasis formation. In conclusion, OACM5 1.C has a poor tumor take rate at an orthotopic and ectopic site. A subpopulation obtained through in vivo selection, OACM5 1.C SC1, gives a significant higher take rate, ectopically. Furthermore, OE33 establishes orthotopic (and subcutaneous) xenografts in mice. These models can be of interest for future studies, and their slow growth rates are a challenge for therapeutic intervention.
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View References

1 

Napier KJ, Scheerer M and Misra S: Esophageal cancer: A Review of epidemiology, pathogenesis, staging workup and treatment modalities. World J Gastrointest Oncol. 6:112–120. 2014. View Article : Google Scholar : PubMed/NCBI

2 

Stahl M, Mariette C, Haustermans K, Cervantes A and Arnold D: ESMO Guidelines Working Group: Oesophageal cancer: ESMO Clinical Practice Guidelines for diagnosis, treatment and follow-up. Ann Oncol. 24 Suppl 6:vi51–vi56. 2013. View Article : Google Scholar : PubMed/NCBI

3 

Gavin AT, Francisci S, Foschi R, Donnelly DW, Lemmens V, Brenner H and Anderson LA: EUROCARE-4 Working Group: Oesophageal cancer survival in Europe: A EUROCARE-4 study. Cancer Epidemiol. 36:505–512. 2012. View Article : Google Scholar : PubMed/NCBI

4 

Tétreault MP: Esophageal cancer: Insights from mouse models. Cancer Growth Metastasis. 8 Suppl 1:S37–S46. 2015. View Article : Google Scholar

5 

Bibby MC: Orthotopic models of cancer for preclinical drug evaluation: Advantages and disadvantages. Eur J Cancer. 40:852–857. 2004. View Article : Google Scholar : PubMed/NCBI

6 

Hanahan D and Weinberg RA: Hallmarks of cancer: The next generation. Cell. 144:646–674. 2011. View Article : Google Scholar : PubMed/NCBI

7 

Kuroda S, Kubota T, Aoyama K, Kikuchi S, Tazawa H, Nishizaki M, Kagawa S and Fujiwara T: Establishment of a non-invasive semi-quantitative bioluminescent imaging method for monitoring of an orthotopic esophageal cancer mouse model. PLoS One. 9:e1145622014. View Article : Google Scholar : PubMed/NCBI

8 

Song S, Chang D, Cui Y, Hu J, Gong M, Ma K, Ding F, Liu ZH and Wang TY: New orthotopic implantation model of human esophageal squamous cell carcinoma in athymic nude mice. Thorac Cancer. 5:417–424. 2014. View Article : Google Scholar : PubMed/NCBI

9 

Habibollahi P, Figueiredo JL, Heidari P, Dulak AM, Imamura Y, Bass AJ, Ogino S, Chan AT and Mahmood U: Optical imaging with a cathepsin B activated probe for the enhanced detection of esophageal adenocarcinoma by dual channel fluorescent upper GI endoscopy. Theranostics. 2:227–234. 2012. View Article : Google Scholar : PubMed/NCBI

10 

Gros SJ, Dohrmann T, Peldschus K, Schurr PG, Kaifi JT, Kalinina T, Reichelt U, Mann O, Strate TG, Adam G, et al: Complementary use of fluorescence and magnetic resonance imaging of metastatic esophageal cancer in a novel orthotopic mouse model. Int J Cancer. 126:2671–2681. 2010.PubMed/NCBI

11 

Drenckhan A, Kurschat N, Dohrmann T, Raabe N, Koenig AM, Reichelt U, Kaifi JT, Izbicki JR and Gros SJ: Effective inhibition of metastases and primary tumor growth with CTCE-9908 in esophageal cancer. J Surg Res. 182:250–256. 2013. View Article : Google Scholar : PubMed/NCBI

12 

Kuroda S, Fujiwara T, Shirakawa Y, Yamasaki Y, Yano S, Uno F, Tazawa H, Hashimoto Y, Watanabe Y, Noma K, et al: Telomerase-dependent oncolytic adenovirus sensitizes human cancer cells to ionizing radiation via inhibition of DNA repair machinery. Cancer Res. 70:9339–9348. 2010. View Article : Google Scholar : PubMed/NCBI

13 

Furihata T, Sakai T, Kawamata H, Omotehara F, Shinagawa Y, Imura J, Ueda Y, Kubota K and Fujimori T: A new in vivo model for studying invasion and metastasis of esophageal squamous cell carcinoma. Int J Oncol. 19:903–907. 2001.PubMed/NCBI

14 

Ip JC, Ko JM, Yu VZ, Chan KW, Lam AK, Law S, Tong DK and Lung ML: A versatile orthotopic nude mouse model for study of esophageal squamous cell carcinoma. Biomed Res Int 910715. 2015. View Article : Google Scholar

15 

Ohara T, Takaoka M, Sakurama K, Nagaishi K, Takeda H, Shirakawa Y, Yamatsuji T, Nagasaka T, Matsuoka J, Tanaka N, et al: The establishment of a new mouse model with orthotopic esophageal cancer showing the esophageal stricture. Cancer Lett. 293:207–212. 2010. View Article : Google Scholar : PubMed/NCBI

16 

Hori T, Yamashita Y, Ohira M, Matsumura Y, Muguruma K and Hirakawa K: A novel orthotopic implantation model of human esophageal carcinoma in nude rats: CD44H mediates cancer cell invasion in vitro and in vivo. Int J Cancer. 92:489–496. 2001. View Article : Google Scholar : PubMed/NCBI

17 

Gros SJ, Kurschat N, Drenckhan A, Dohrmann T, Forberich E, Effenberger K, Reichelt U, Hoffman RM, Pantel K, Kaifi JT, et al: Involvement of CXCR4 chemokine receptor in metastastic HER2-positive esophageal cancer. PLoS One. 7:e472872012. View Article : Google Scholar : PubMed/NCBI

18 

Gros SJ, Kurschat N, Dohrmann T, Reichelt U, Dancau AM, Peldschus K, Adam G, Hoffman RM, Izbicki JR and Kaifi JT: Effective therapeutic targeting of the overexpressed HER-2 receptor in a highly metastatic orthotopic model of esophageal carcinoma. Mol Cancer Ther. 9:2037–2045. 2010. View Article : Google Scholar : PubMed/NCBI

19 

Gros SJ, Dohrmann T, Rawnaq T, Kurschat N, Bouvet M, Wessels J, Hoffmann RM, Izbicki JR and Kaifi JT: Orthotopic fluorescent peritoneal carcinomatosis model of esophageal cancer. Anticancer Res. 30:3933–3938. 2010.PubMed/NCBI

20 

Castro C, Bosetti C, Malvezzi M, Bertuccio P, Levi F, Negri E, La Vecchia C and Lunet N: Patterns and trends in esophageal cancer mortality and incidence in Europe (1980–2011) and predictions to 2015. Ann Oncol. 25:283–290. 2014. View Article : Google Scholar : PubMed/NCBI

21 

Almhanna K, Meredith KL, Hoffe SE, Shridhar R and Coppola D: Targeting the human epidermal growth factor receptor 2 in esophageal cancer. Cancer Control. 20:111–116. 2013.PubMed/NCBI

22 

De Wever O, Hendrix A, De Boeck A, Westbroek W, Braems G, Emami S, Sabbah M, Gespach C and Bracke M: Modeling and quantification of cancer cell invasion through collagen type I matrices. Int J Dev Biol. 54:887–896. 2010. View Article : Google Scholar : PubMed/NCBI

23 

Raggi M, Langer R, Feith M, Friess H, Schauer M and Theisen J: Successful evaluation of a new animal model using mice for esophageal adenocarcinoma. Langenbecks Arch Surg. 395:347–350. 2010. View Article : Google Scholar : PubMed/NCBI

24 

Quante M, Bhagat G, Abrams JA, Marache F, Good P, Lee MD, Lee Y, Friedman R, Asfaha S, Dubeykovskaya Z, et al: Bile acid and inflammation activate gastric cardia stem cells in a mouse model of Barrett-like metaplasia. Cancer Cell. 21:36–51. 2012. View Article : Google Scholar : PubMed/NCBI

25 

De Vlieghere E, Carlier C, Ceelen W, Bracke M and De Wever O: Data on in vivo selection of SK-OV-3 Luc ovarian cancer cells and intraperitoneal tumor formation with low inoculation numbers. Data Brief. 6:542–549. 2016. View Article : Google Scholar : PubMed/NCBI

26 

Minn AJ, Gupta GP, Siegel PM, Bos PD, Shu W, Giri DD, Viale A, Olshen AB, Gerald WL and Massagué J: Genes that mediate breast cancer metastasis to lung. Nature. 436:518–524. 2005. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Melsens E, De Vlieghere E, Descamps B, Vanhove C, De Wever O, Ceelen W and Pattyn P: Improved xenograft efficiency of esophageal adenocarcinoma cell lines through in vivo selection. Oncol Rep 38: 71-81, 2017.
APA
Melsens, E., De Vlieghere, E., Descamps, B., Vanhove, C., De Wever, O., Ceelen, W., & Pattyn, P. (2017). Improved xenograft efficiency of esophageal adenocarcinoma cell lines through in vivo selection. Oncology Reports, 38, 71-81. https://doi.org/10.3892/or.2017.5640
MLA
Melsens, E., De Vlieghere, E., Descamps, B., Vanhove, C., De Wever, O., Ceelen, W., Pattyn, P."Improved xenograft efficiency of esophageal adenocarcinoma cell lines through in vivo selection". Oncology Reports 38.1 (2017): 71-81.
Chicago
Melsens, E., De Vlieghere, E., Descamps, B., Vanhove, C., De Wever, O., Ceelen, W., Pattyn, P."Improved xenograft efficiency of esophageal adenocarcinoma cell lines through in vivo selection". Oncology Reports 38, no. 1 (2017): 71-81. https://doi.org/10.3892/or.2017.5640
Copy and paste a formatted citation
x
Spandidos Publications style
Melsens E, De Vlieghere E, Descamps B, Vanhove C, De Wever O, Ceelen W and Pattyn P: Improved xenograft efficiency of esophageal adenocarcinoma cell lines through in vivo selection. Oncol Rep 38: 71-81, 2017.
APA
Melsens, E., De Vlieghere, E., Descamps, B., Vanhove, C., De Wever, O., Ceelen, W., & Pattyn, P. (2017). Improved xenograft efficiency of esophageal adenocarcinoma cell lines through in vivo selection. Oncology Reports, 38, 71-81. https://doi.org/10.3892/or.2017.5640
MLA
Melsens, E., De Vlieghere, E., Descamps, B., Vanhove, C., De Wever, O., Ceelen, W., Pattyn, P."Improved xenograft efficiency of esophageal adenocarcinoma cell lines through in vivo selection". Oncology Reports 38.1 (2017): 71-81.
Chicago
Melsens, E., De Vlieghere, E., Descamps, B., Vanhove, C., De Wever, O., Ceelen, W., Pattyn, P."Improved xenograft efficiency of esophageal adenocarcinoma cell lines through in vivo selection". Oncology Reports 38, no. 1 (2017): 71-81. https://doi.org/10.3892/or.2017.5640
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