Functional significance of the long non-coding RNA RP11-169D4.1 as a metastasis suppressor in laryngeal squamous cell carcinoma by regulating CDH1

  • Authors:
    • Jing Zhao
    • Kexing Lv
    • Zeng-Hong Li
    • Jianhui Wu
    • Wei Gao
    • Thian-Sze Wong
    • Jie Luo
    • Hao Qin
    • Bin Wang
    • Qingling Fu
    • Wen-Bin Lei
  • View Affiliations

  • Published online on: May 16, 2017     https://doi.org/10.3892/or.2017.5645
  • Pages: 211-220
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Abstract

The present study investigated the expression profile and the function of RP11-169D4.1 and explored its potential mechanisms in laryngeal squamous cell carcinoma. The biological function of RP11-169D4.1 was examined using the MTT assay, flow cytometric analysis, wound healing and transwell assays. The relationship between RP11-169D4.1 and miR-205-5p was discovered by Argonaute 2 protein immunoprecipitation. The target gene of RP11-169D4.1 was CDH1 which was assessed by Pearson's correlation analysis, RT-PCR and western blot assay. We demonstrated that RP11-169D4.1 expression was markedly decreased in LSCC tissues and cell lines. The overexpression of RP11-169D4.1 inhibited the proliferation, migration and invasion of LSCC cell lines as well as promoted apoptosis. We further verified that miR-205-5p had binding sites with RP11‑169D4.1 and that RP11-169D4.1 could regulate the expression of CDH1. Ectopic transfection of RP11-169D4.1 led to a significant reduction in the downstream signaling molecule AKT in LSCC cells. The long non-coding RNA RP11-169D4.1 may serve as a tumor suppressor and a promising therapeutic target in laryngeal cancer, which could inhibit the process of EMT by regulating CDH1.
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July-2017
Volume 38 Issue 1

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Zhao J, Lv K, Li Z, Wu J, Gao W, Wong T, Luo J, Qin H, Wang B, Fu Q, Fu Q, et al: Functional significance of the long non-coding RNA RP11-169D4.1 as a metastasis suppressor in laryngeal squamous cell carcinoma by regulating CDH1. Oncol Rep 38: 211-220, 2017.
APA
Zhao, J., Lv, K., Li, Z., Wu, J., Gao, W., Wong, T. ... Lei, W. (2017). Functional significance of the long non-coding RNA RP11-169D4.1 as a metastasis suppressor in laryngeal squamous cell carcinoma by regulating CDH1. Oncology Reports, 38, 211-220. https://doi.org/10.3892/or.2017.5645
MLA
Zhao, J., Lv, K., Li, Z., Wu, J., Gao, W., Wong, T., Luo, J., Qin, H., Wang, B., Fu, Q., Lei, W."Functional significance of the long non-coding RNA RP11-169D4.1 as a metastasis suppressor in laryngeal squamous cell carcinoma by regulating CDH1". Oncology Reports 38.1 (2017): 211-220.
Chicago
Zhao, J., Lv, K., Li, Z., Wu, J., Gao, W., Wong, T., Luo, J., Qin, H., Wang, B., Fu, Q., Lei, W."Functional significance of the long non-coding RNA RP11-169D4.1 as a metastasis suppressor in laryngeal squamous cell carcinoma by regulating CDH1". Oncology Reports 38, no. 1 (2017): 211-220. https://doi.org/10.3892/or.2017.5645