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Article

HIF-1α inhibits IDH-1 expression in osteosarcoma

  • Authors:
    • Deng-Cheng Liu
    • Xun Zheng
    • Yong Zho
    • Wan-Rong Yi
    • Zong-Huan Li
    • Xiang Hu
    • Ai-Xi Yu
  • View Affiliations / Copyright

    Affiliations: Zhongnan Hospital of Wuhan University, Department of Orthopedics, Wuhan University, Wuhan, Hubei 430071, P.R. China
  • Pages: 336-342
    |
    Published online on: May 22, 2017
       https://doi.org/10.3892/or.2017.5655
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Abstract

Recently, hypoxia inducible factor-1 (HIF-1) was reported to be correlated with isocitrate dehydrogenase 1 (IDH-1) in several types of tumors. However, the expression and significance of HIF-1 and IDH-1 in osteosarcoma is still unknown. In the present study, the expression levels of IDH-1 and HIF-1α in 35 formalin-fixed paraffin-embedded sections from osteosarcoma patients were investigated by immunohistochemistry. The expression levels of IDH-1 and HIF-1α in human osteosarcoma cell lines (MG-63 and 143B) were further detected by western blotting under normal and hypoxic conditions. In addition, HIF-1α was downregulated via lentiviral vector‑mediated RNA interference (RNAi) in the MG-63 human osteosarcoma cell line. The results revealed that HIF-1α was negatively correlated with IDH-1 in the osteosarcoma tissues. Both in MG-63 and 143B cell lines, the expression of HIF-1α was increased while IDH-1 was decreased under a hypoxic condition compared to normal conditions. HIF-1α downregulation promoted IDH-1 expression in the MG-63 cell line under either normal or hypoxic conditions. In conclusion, our findings suggest that HIF-1α inhibits IDH-1 in osteosarcoma and consequently increases the incidence of osteosarcoma.
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Copy and paste a formatted citation
Spandidos Publications style
Liu D, Zheng X, Zho Y, Yi W, Li Z, Hu X and Yu A: HIF-1α inhibits IDH-1 expression in osteosarcoma. Oncol Rep 38: 336-342, 2017.
APA
Liu, D., Zheng, X., Zho, Y., Yi, W., Li, Z., Hu, X., & Yu, A. (2017). HIF-1α inhibits IDH-1 expression in osteosarcoma. Oncology Reports, 38, 336-342. https://doi.org/10.3892/or.2017.5655
MLA
Liu, D., Zheng, X., Zho, Y., Yi, W., Li, Z., Hu, X., Yu, A."HIF-1α inhibits IDH-1 expression in osteosarcoma". Oncology Reports 38.1 (2017): 336-342.
Chicago
Liu, D., Zheng, X., Zho, Y., Yi, W., Li, Z., Hu, X., Yu, A."HIF-1α inhibits IDH-1 expression in osteosarcoma". Oncology Reports 38, no. 1 (2017): 336-342. https://doi.org/10.3892/or.2017.5655
Copy and paste a formatted citation
x
Spandidos Publications style
Liu D, Zheng X, Zho Y, Yi W, Li Z, Hu X and Yu A: HIF-1α inhibits IDH-1 expression in osteosarcoma. Oncol Rep 38: 336-342, 2017.
APA
Liu, D., Zheng, X., Zho, Y., Yi, W., Li, Z., Hu, X., & Yu, A. (2017). HIF-1α inhibits IDH-1 expression in osteosarcoma. Oncology Reports, 38, 336-342. https://doi.org/10.3892/or.2017.5655
MLA
Liu, D., Zheng, X., Zho, Y., Yi, W., Li, Z., Hu, X., Yu, A."HIF-1α inhibits IDH-1 expression in osteosarcoma". Oncology Reports 38.1 (2017): 336-342.
Chicago
Liu, D., Zheng, X., Zho, Y., Yi, W., Li, Z., Hu, X., Yu, A."HIF-1α inhibits IDH-1 expression in osteosarcoma". Oncology Reports 38, no. 1 (2017): 336-342. https://doi.org/10.3892/or.2017.5655
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