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Article Open Access

Upregulation of miR-101 enhances the cytotoxic effect of anticancer drugs through inhibition of colon cancer cell proliferation

  • Authors:
    • Le-Gao Chen
    • Ying-Jie Xia
    • Ying Cui
  • View Affiliations / Copyright

    Affiliations: Department of Vascular Surgery, Zhejiang Provincial People's Hospital, Hangzhou, Zhejiang 310014, P.R. China, Key Laboratory of Gastroenterology, Zhejiang Provincial People's Hospital, Hangzhou, Zhejiang 310014, P.R. China, Department of Unclear Medicine, Zhejiang Provincial People's Hospital, Hangzhou, Zhejiang 310014, P.R. China
    Copyright: © Chen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 100-108
    |
    Published online on: May 24, 2017
       https://doi.org/10.3892/or.2017.5666
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Abstract

This study investigated the effect of miR-101 on proliferation, migration, invasion, and chemotherapy sensitivity in colon cancer cell lines HT-29 and RKO. MicroRNAs are a class of small noncoding RNA molecules, which play important roles in diverse biological processes of human cancers, such as carcinogenesis, development, differentiation, and apoptosis. The expression of miR-101 in colon cancer and adjacent non-tumor tissues were examined by quantitative real-time polymerase chain reaction. The expression of miR-101 was upregulated by recombinant adenovirus Ad-miR-101. Cell proliferation was detected by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and cloning methods. Cell migration and invasion potential were examined using Transwell migration and Matrigel invasion chamber assays. Drug sensitivity to 5-fluorouracil (5-FU) and cisplatin (DDP) was explored using MTT assays and l acridine orange/ethidium bromide double staining. The expression of miR-101 decreased in colon cancer tissues compared with adjacent non-tumor tissues. The upregulated expression of miR-101 suppressed cell proliferation and inhibited cell migration and invasion in HT-29 and RKO colon cancer cell lines. The overexpression of miR-101 promoted the inhibitory effect of 5-FU and DDP on HT-29 cells. The expression of miR-101 was downregulated in colon cancer. The upregulated expression of miR-101 inhibited proliferation and migration, and increased the sensitivity of colon cancer cells to chemotherapy.
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Copy and paste a formatted citation
Spandidos Publications style
Chen L, Xia Y and Cui Y: Upregulation of miR-101 enhances the cytotoxic effect of anticancer drugs through inhibition of colon cancer cell proliferation. Oncol Rep 38: 100-108, 2017.
APA
Chen, L., Xia, Y., & Cui, Y. (2017). Upregulation of miR-101 enhances the cytotoxic effect of anticancer drugs through inhibition of colon cancer cell proliferation. Oncology Reports, 38, 100-108. https://doi.org/10.3892/or.2017.5666
MLA
Chen, L., Xia, Y., Cui, Y."Upregulation of miR-101 enhances the cytotoxic effect of anticancer drugs through inhibition of colon cancer cell proliferation". Oncology Reports 38.1 (2017): 100-108.
Chicago
Chen, L., Xia, Y., Cui, Y."Upregulation of miR-101 enhances the cytotoxic effect of anticancer drugs through inhibition of colon cancer cell proliferation". Oncology Reports 38, no. 1 (2017): 100-108. https://doi.org/10.3892/or.2017.5666
Copy and paste a formatted citation
x
Spandidos Publications style
Chen L, Xia Y and Cui Y: Upregulation of miR-101 enhances the cytotoxic effect of anticancer drugs through inhibition of colon cancer cell proliferation. Oncol Rep 38: 100-108, 2017.
APA
Chen, L., Xia, Y., & Cui, Y. (2017). Upregulation of miR-101 enhances the cytotoxic effect of anticancer drugs through inhibition of colon cancer cell proliferation. Oncology Reports, 38, 100-108. https://doi.org/10.3892/or.2017.5666
MLA
Chen, L., Xia, Y., Cui, Y."Upregulation of miR-101 enhances the cytotoxic effect of anticancer drugs through inhibition of colon cancer cell proliferation". Oncology Reports 38.1 (2017): 100-108.
Chicago
Chen, L., Xia, Y., Cui, Y."Upregulation of miR-101 enhances the cytotoxic effect of anticancer drugs through inhibition of colon cancer cell proliferation". Oncology Reports 38, no. 1 (2017): 100-108. https://doi.org/10.3892/or.2017.5666
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