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Article Open Access

GROα overexpression drives cell migration and invasion in triple negative breast cancer cells

  • Authors:
    • Kruttika Bhat
    • Marianna Sarkissyan
    • Yanyuan Wu
    • Jaydutt V. Vadgama
  • View Affiliations / Copyright

    Affiliations: Division of Cancer Research and Training, Department of Internal Medicine, Center to Eliminate Cancer Health Disparities, Charles R. Drew University of Medicine and Science, Los Angeles, CA 90059, USA
    Copyright: © Bhat et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 21-30
    |
    Published online on: May 24, 2017
       https://doi.org/10.3892/or.2017.5668
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Abstract

Triple negative breast cancer (TNBC) is a subtype of highly aggressive breast cancer with poor prognosis. The main characteristic feature of TNBC is its lack of expression of ER, PR and HER2 receptors that are targets for treatments. Hence, it is imperative to identify novel therapeutic strategies to target TNBC. Our aim was to examine whether GROα is a specific marker for TNBC metastasis. For this we performed qPCR, ELISA, migration/invasion assays, western blotting, and siRNA transfections. Evaluation of baseline GROα expression in different breast cancer (BC) subtypes showed that it is significantly upregulated in breast tumor cells, specifically in TNBC cell line. On further evaluation in additional 17 TNBC cell lines we found that baseline GROα expression was significantly elevated in >50% of the cell lines validating GROα overexpression specifically in TNBC cells. Moreover, GROα-stimulation in MCF7 and SKBR3 cells and GROα‑knockdown in MDA-MB‑231 and HCC1937 cells elicited dramatic changes in migration and invasion abilities in vitro. Corresponding changes in EMT markers were also observed in phenotypically modified BC cells. Furthermore, mechanistic studies identified GROα regulating EMT markers and migration/invasion via MAPK pathway and specific inhibition using PD98059 resulted in the reversal of effects induced by GROα on BC cells. In conclusion, our study provides strong evidence to suggest that GROα is a critical modulator of TNBC migration/invasion and proposes GROα as a potential therapeutic target for treatment of TNBC metastasis.
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Copy and paste a formatted citation
Spandidos Publications style
Bhat K, Sarkissyan M, Wu Y and Vadgama JV: GROα overexpression drives cell migration and invasion in triple negative breast cancer cells. Oncol Rep 38: 21-30, 2017.
APA
Bhat, K., Sarkissyan, M., Wu, Y., & Vadgama, J.V. (2017). GROα overexpression drives cell migration and invasion in triple negative breast cancer cells. Oncology Reports, 38, 21-30. https://doi.org/10.3892/or.2017.5668
MLA
Bhat, K., Sarkissyan, M., Wu, Y., Vadgama, J. V."GROα overexpression drives cell migration and invasion in triple negative breast cancer cells". Oncology Reports 38.1 (2017): 21-30.
Chicago
Bhat, K., Sarkissyan, M., Wu, Y., Vadgama, J. V."GROα overexpression drives cell migration and invasion in triple negative breast cancer cells". Oncology Reports 38, no. 1 (2017): 21-30. https://doi.org/10.3892/or.2017.5668
Copy and paste a formatted citation
x
Spandidos Publications style
Bhat K, Sarkissyan M, Wu Y and Vadgama JV: GROα overexpression drives cell migration and invasion in triple negative breast cancer cells. Oncol Rep 38: 21-30, 2017.
APA
Bhat, K., Sarkissyan, M., Wu, Y., & Vadgama, J.V. (2017). GROα overexpression drives cell migration and invasion in triple negative breast cancer cells. Oncology Reports, 38, 21-30. https://doi.org/10.3892/or.2017.5668
MLA
Bhat, K., Sarkissyan, M., Wu, Y., Vadgama, J. V."GROα overexpression drives cell migration and invasion in triple negative breast cancer cells". Oncology Reports 38.1 (2017): 21-30.
Chicago
Bhat, K., Sarkissyan, M., Wu, Y., Vadgama, J. V."GROα overexpression drives cell migration and invasion in triple negative breast cancer cells". Oncology Reports 38, no. 1 (2017): 21-30. https://doi.org/10.3892/or.2017.5668
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