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Article

miR-30e acts as a tumor suppressor in hepatocellular carcinoma partly via JAK1/STAT3 pathway

  • Authors:
    • Junjie Mao
    • Xiaojun Hu
    • Pengfei Pang
    • Bin Zhou
    • Dan Li
    • Hong Shan
  • View Affiliations / Copyright

    Affiliations: Center for Interventional Medicine, The Fifth Affiliated Hospital of Sun Yat-sen University, Zhuhai, Guangdong 519000, P.R. China, Guangdong Provincial Engineering Research Center of Molecular Imaging, The Fifth Affiliated Hospital of Sun Yat-sen University, Zhuhai, Guangdong 519000, P.R. China
  • Pages: 393-401
    |
    Published online on: May 30, 2017
       https://doi.org/10.3892/or.2017.5683
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Abstract

Hepatocellular carcinoma (HCC) is the leading cause of cancer-associated mortalities. The effective diagnostic and therapeutic targets for HCC are still unclear. miR-30e was differentially expressed in the majority of HCC tissues and cell lines. The aim of this study was to investigate the functional roles of miR-30e and their modulation of cancer networks in HCC cells. We determined the expression of miR-30e by quantitative real-time polymerase chain reaction, and found downregulation of miR-30e in HepG2 and HuH7 cells. miR-30e mimics significantly inhibited the proliferation, migration, and invasion of HepG2 and HuH7 cells, and promoted cell apoptosis, but did not influence the cell cycle. Dual-luciferase reporter assays were applied to identify JAK1 as target of miR-30e. miR-30e mimics downregulated the expression levels of JAK1 and vimentin in mRNA and protein in HepG2 and HuH7 cells. Silence of JAK1 by small interfering RNAs inhibited cell proliferation, migration and invasion of HCC cells. Furthermore, we verified that, IL-6, an agonist of JAK1/STAT3 pathway partly recovered the inhibition of miR-30e mimics on cell migration. Taken together, these findings confirmed our speculation that the functional effect of miR-30e on HCC cells, in part, is dependent on the JAK1/STAT3 signaling pathway. It was suggested that miR-30e has a critical role in the suppression of HCC and presents a novel mechanism of miRNA-mediated JAK1 expression in cancer cells that might be a good prognostic marker for survival of HCC patients.
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Copy and paste a formatted citation
Spandidos Publications style
Mao J, Hu X, Pang P, Zhou B, Li D and Shan H: miR-30e acts as a tumor suppressor in hepatocellular carcinoma partly via JAK1/STAT3 pathway. Oncol Rep 38: 393-401, 2017.
APA
Mao, J., Hu, X., Pang, P., Zhou, B., Li, D., & Shan, H. (2017). miR-30e acts as a tumor suppressor in hepatocellular carcinoma partly via JAK1/STAT3 pathway. Oncology Reports, 38, 393-401. https://doi.org/10.3892/or.2017.5683
MLA
Mao, J., Hu, X., Pang, P., Zhou, B., Li, D., Shan, H."miR-30e acts as a tumor suppressor in hepatocellular carcinoma partly via JAK1/STAT3 pathway". Oncology Reports 38.1 (2017): 393-401.
Chicago
Mao, J., Hu, X., Pang, P., Zhou, B., Li, D., Shan, H."miR-30e acts as a tumor suppressor in hepatocellular carcinoma partly via JAK1/STAT3 pathway". Oncology Reports 38, no. 1 (2017): 393-401. https://doi.org/10.3892/or.2017.5683
Copy and paste a formatted citation
x
Spandidos Publications style
Mao J, Hu X, Pang P, Zhou B, Li D and Shan H: miR-30e acts as a tumor suppressor in hepatocellular carcinoma partly via JAK1/STAT3 pathway. Oncol Rep 38: 393-401, 2017.
APA
Mao, J., Hu, X., Pang, P., Zhou, B., Li, D., & Shan, H. (2017). miR-30e acts as a tumor suppressor in hepatocellular carcinoma partly via JAK1/STAT3 pathway. Oncology Reports, 38, 393-401. https://doi.org/10.3892/or.2017.5683
MLA
Mao, J., Hu, X., Pang, P., Zhou, B., Li, D., Shan, H."miR-30e acts as a tumor suppressor in hepatocellular carcinoma partly via JAK1/STAT3 pathway". Oncology Reports 38.1 (2017): 393-401.
Chicago
Mao, J., Hu, X., Pang, P., Zhou, B., Li, D., Shan, H."miR-30e acts as a tumor suppressor in hepatocellular carcinoma partly via JAK1/STAT3 pathway". Oncology Reports 38, no. 1 (2017): 393-401. https://doi.org/10.3892/or.2017.5683
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