Effects of galectin-9 on apoptosis, cell cycle and autophagy in human esophageal adenocarcinoma cells

  • Authors:
    • Emiko Akashi
    • Shintaro Fujihara
    • Asahiro Morishita
    • Tomoko Tadokoro
    • Taiga Chiyo
    • Keiko Fujikawa
    • Hideki Kobara
    • Hirohito Mori
    • Hisakazu Iwama
    • Keiichi Okano
    • Yasuyuki Suzuki
    • Toshiro Niki
    • Mitsuomi Hirashima
    • Tsutomu Masaki
  • View Affiliations

  • Published online on: June 1, 2017     https://doi.org/10.3892/or.2017.5689
  • Pages: 506-514
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Abstract

The incidence of esophageal adenocarcinoma (EAC) is rapidly increasing in western countries. The overall mortality of this disease remains high with a 5-year survival rate of less than 20%, despite remarkable advances in the care of patients with EAC. Galectin-9 (Gal-9) is a tandem-repeat type galectin that exerts anti-proliferative effects on various cancer cell types. The aim of the present study was to evaluate the effects of Gal-9 on human EAC cells and to assess the expression of microRNAs (miRNAs) associated with the antitumor effects of Gal-9 in vitro. Gal-9 suppressed the proliferation of the EAC cell lines OE19, OE33, SK-GT4, and OACM 5.1C. Additionally, Gal-9 treatment induced apoptosis and increased the expression levels of caspase-cleaved cytokeratin 18, activated caspase-3 and activated caspase-9. However, it did not promote cell cycle arrest by reducing cell cycle-related protein levels. Furthermore, Gal-9 increased the level of the angiogenesis-related protein interleukin-8 (IL-8) and markedly altered miRNA expression. Based on these findings, Gal-9 may be of clinical use for the treatment of EAC.
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July-2017
Volume 38 Issue 1

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Akashi E, Fujihara S, Morishita A, Tadokoro T, Chiyo T, Fujikawa K, Kobara H, Mori H, Iwama H, Okano K, Okano K, et al: Effects of galectin-9 on apoptosis, cell cycle and autophagy in human esophageal adenocarcinoma cells. Oncol Rep 38: 506-514, 2017
APA
Akashi, E., Fujihara, S., Morishita, A., Tadokoro, T., Chiyo, T., Fujikawa, K. ... Masaki, T. (2017). Effects of galectin-9 on apoptosis, cell cycle and autophagy in human esophageal adenocarcinoma cells. Oncology Reports, 38, 506-514. https://doi.org/10.3892/or.2017.5689
MLA
Akashi, E., Fujihara, S., Morishita, A., Tadokoro, T., Chiyo, T., Fujikawa, K., Kobara, H., Mori, H., Iwama, H., Okano, K., Suzuki, Y., Niki, T., Hirashima, M., Masaki, T."Effects of galectin-9 on apoptosis, cell cycle and autophagy in human esophageal adenocarcinoma cells". Oncology Reports 38.1 (2017): 506-514.
Chicago
Akashi, E., Fujihara, S., Morishita, A., Tadokoro, T., Chiyo, T., Fujikawa, K., Kobara, H., Mori, H., Iwama, H., Okano, K., Suzuki, Y., Niki, T., Hirashima, M., Masaki, T."Effects of galectin-9 on apoptosis, cell cycle and autophagy in human esophageal adenocarcinoma cells". Oncology Reports 38, no. 1 (2017): 506-514. https://doi.org/10.3892/or.2017.5689