Therapy-relevant aberrant expression of MRP3 and BCRP mRNA in TCC-/SCC-bladder cancer tissue of untreated patients

  • Authors:
    • Mona Rady
    • Marwa Mostageer
    • Jan Rohde
    • Ashraf Zaghloul
    • Ruth Knüchel-Clarke
    • Shady Saad
    • Deena Attia
    • Laila Mahran
    • Hilde Spahn-Langguth
  • View Affiliations

  • Published online on: June 2, 2017     https://doi.org/10.3892/or.2017.5695
  • Pages: 551-560
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Abstract

Multidrug resistance (MDR) is a critical factor, which results in suboptimal outcomes in cancer chemotherapy. One principal mechanism of MDR is the increased expression of ATP-binding cassette (ABC) transporters. Of these, multidrug resistance-associated protein 3 (MRP3) and breast cancer resistance protein (BCRP) confer MDR when overexpressed in cancer cell lines. We measured the mRNA expression of MRP3 and BCRP in primary untreated bladder cancer specimens using reverse transcription-quantitative PCR (RT-qPCR) in comparison to normal bladder tissue. The MRP3 and BCRP expression in the two major histotypes of bladder cancer; transitional cell carcinoma (TCC; urothelial type of bladder cancer) and squamous cell carcinoma (SCC; ‘Schistosoma-induced’ bladder cancer) were compared. Furthermore, the association between MRP3 and BCRP expression and tumor grade and stage were investigated. MRP3 mRNA expression in bladder cancer specimens was increased ~13-fold on average compared to normal bladder tissue (n=36, P<0.0001). BCRP mRNA expression was decreased in bladder cancer specimens to ~1/5 on average, compared to normal bladder tissue (n=38, P<0.0001). TCC showed significantly increased MRP3 mRNA expression compared to SCC of the bladder (P<0.0001). BCRP mRNA expression was similar in TCC and SCC of the bladder (P=0.1072). The increased MRP3 mRNA expression was not related to bladder tumor grade (P=0.3465) but was, however, significantly higher in superficial than in invasive bladder tumors (P=0.0173). The decreased expression of BCRP was not related to bladder tumor grade (P=0.1808) or stage (P=0.8016). The current data show that bladder cancer is associated with perturbed expression of MRP3 and BCRP. Representing drug resistance factors, determining the expression of these transporters in native tumors may be predictive of the outcome of chemotherapy based-treatment of bladder cancer.
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July-2017
Volume 38 Issue 1

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Rady M, Mostageer M, Rohde J, Zaghloul A, Knüchel-Clarke R, Saad S, Attia D, Mahran L and Spahn-Langguth H: Therapy-relevant aberrant expression of MRP3 and BCRP mRNA in TCC-/SCC-bladder cancer tissue of untreated patients. Oncol Rep 38: 551-560, 2017
APA
Rady, M., Mostageer, M., Rohde, J., Zaghloul, A., Knüchel-Clarke, R., Saad, S. ... Spahn-Langguth, H. (2017). Therapy-relevant aberrant expression of MRP3 and BCRP mRNA in TCC-/SCC-bladder cancer tissue of untreated patients. Oncology Reports, 38, 551-560. https://doi.org/10.3892/or.2017.5695
MLA
Rady, M., Mostageer, M., Rohde, J., Zaghloul, A., Knüchel-Clarke, R., Saad, S., Attia, D., Mahran, L., Spahn-Langguth, H."Therapy-relevant aberrant expression of MRP3 and BCRP mRNA in TCC-/SCC-bladder cancer tissue of untreated patients". Oncology Reports 38.1 (2017): 551-560.
Chicago
Rady, M., Mostageer, M., Rohde, J., Zaghloul, A., Knüchel-Clarke, R., Saad, S., Attia, D., Mahran, L., Spahn-Langguth, H."Therapy-relevant aberrant expression of MRP3 and BCRP mRNA in TCC-/SCC-bladder cancer tissue of untreated patients". Oncology Reports 38, no. 1 (2017): 551-560. https://doi.org/10.3892/or.2017.5695