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Article

Overexpression of microRNA-133b is associated with the increased survival of patients with hepatocellular carcinoma after curative hepatectomy: Involvement of the EGFR/PI3K/Akt/mTOR signaling pathway

  • Authors:
    • Xuedong Wang
    • Jianping Zeng
    • Liang Wang
    • Xinjing Zhang
    • Zhiqian Liu
    • Hongyi Zhang
    • Jiahong Dong
  • View Affiliations / Copyright

    Affiliations: Department of Hepatobiliary Surgery, Beijing Tsinghua Changgung Hospital, Medical Center, Tsinghua University, Beijing 102218, P.R. China, Medical School of Tsinghua University, Beijing 100084, P.R. China, Department of Hepatobiliary Surgery, Chinese General PLA Hospital, Beijing 100853, P.R. China
  • Pages: 141-150
    |
    Published online on: June 6, 2017
       https://doi.org/10.3892/or.2017.5699
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Abstract

The aim of the present study was carried out to investigate the association of microRNA-133b (miRNA-133b) expression with the prognosis of patients with hepatocellular carcinoma (HCC) after curative hepatectomy. In the present study, the expression of miRNA-133b in HCC tissues was determined to be lower than that noted in the adjacent normal tissues. Overall and disease-free survival of the HCC patients with high miRNA-133b expression was observably extended, compared with the HCC patients with low miRNA‑133b expression. Moreover, the overexpression of miRNA-133b inhibited cell proliferation, increased lactate dehydrogenase (LDH) activity, induced apoptosis and promoted caspase‑3/-8 activities and Bax/Bcl-2 protein expression in HCC cells, whereas the protein expression of epidermal growth factor receptor (EGFR) was significantly decreased. The overexpression of miRNA-133b significantly suppressed PI3K, phosphorylated (p)-Akt and p-mTOR protein expression in HCC cells. GW2974, an EGFR inhibitor, suppressed the protein expression of EGFR, inhibited cell proliferation, increased LDH activity, induced apoptosis and promoted caspase-3/-8 activities and Bax/Bcl-2 protein expression, downregulated PI3K, p-Akt and p-mTOR protein expression in the transfected HCC cells overexpressing miRNA-133b. Taken together, our results indicate that the overexpression of miRNA-133b is associated with the increased survival of HCC patients after curative hepatectomy. The effects of miRNA-133b in HCC are mediated through the EGFR/PI3K/Akt/mTOR signaling pathway.
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Copy and paste a formatted citation
Spandidos Publications style
Wang X, Zeng J, Wang L, Zhang X, Liu Z, Zhang H and Dong J: Overexpression of microRNA-133b is associated with the increased survival of patients with hepatocellular carcinoma after curative hepatectomy: Involvement of the EGFR/PI3K/Akt/mTOR signaling pathway. Oncol Rep 38: 141-150, 2017.
APA
Wang, X., Zeng, J., Wang, L., Zhang, X., Liu, Z., Zhang, H., & Dong, J. (2017). Overexpression of microRNA-133b is associated with the increased survival of patients with hepatocellular carcinoma after curative hepatectomy: Involvement of the EGFR/PI3K/Akt/mTOR signaling pathway. Oncology Reports, 38, 141-150. https://doi.org/10.3892/or.2017.5699
MLA
Wang, X., Zeng, J., Wang, L., Zhang, X., Liu, Z., Zhang, H., Dong, J."Overexpression of microRNA-133b is associated with the increased survival of patients with hepatocellular carcinoma after curative hepatectomy: Involvement of the EGFR/PI3K/Akt/mTOR signaling pathway". Oncology Reports 38.1 (2017): 141-150.
Chicago
Wang, X., Zeng, J., Wang, L., Zhang, X., Liu, Z., Zhang, H., Dong, J."Overexpression of microRNA-133b is associated with the increased survival of patients with hepatocellular carcinoma after curative hepatectomy: Involvement of the EGFR/PI3K/Akt/mTOR signaling pathway". Oncology Reports 38, no. 1 (2017): 141-150. https://doi.org/10.3892/or.2017.5699
Copy and paste a formatted citation
x
Spandidos Publications style
Wang X, Zeng J, Wang L, Zhang X, Liu Z, Zhang H and Dong J: Overexpression of microRNA-133b is associated with the increased survival of patients with hepatocellular carcinoma after curative hepatectomy: Involvement of the EGFR/PI3K/Akt/mTOR signaling pathway. Oncol Rep 38: 141-150, 2017.
APA
Wang, X., Zeng, J., Wang, L., Zhang, X., Liu, Z., Zhang, H., & Dong, J. (2017). Overexpression of microRNA-133b is associated with the increased survival of patients with hepatocellular carcinoma after curative hepatectomy: Involvement of the EGFR/PI3K/Akt/mTOR signaling pathway. Oncology Reports, 38, 141-150. https://doi.org/10.3892/or.2017.5699
MLA
Wang, X., Zeng, J., Wang, L., Zhang, X., Liu, Z., Zhang, H., Dong, J."Overexpression of microRNA-133b is associated with the increased survival of patients with hepatocellular carcinoma after curative hepatectomy: Involvement of the EGFR/PI3K/Akt/mTOR signaling pathway". Oncology Reports 38.1 (2017): 141-150.
Chicago
Wang, X., Zeng, J., Wang, L., Zhang, X., Liu, Z., Zhang, H., Dong, J."Overexpression of microRNA-133b is associated with the increased survival of patients with hepatocellular carcinoma after curative hepatectomy: Involvement of the EGFR/PI3K/Akt/mTOR signaling pathway". Oncology Reports 38, no. 1 (2017): 141-150. https://doi.org/10.3892/or.2017.5699
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