Open Access

Distinctive crypt shape in a sessile serrated adenoma/polyp: Distribution of Ki67-, p16INK4a-, WNT5A-positive cells and intraepithelial lymphocytes

  • Authors:
    • Kenji Hisamatsu
    • Kei Noguchi
    • Hiroyuki Tomita
    • Aoi Muto
    • Natsumi Yamada
    • Kazuhiro Kobayashi
    • Akihiro Hirata
    • Tomohiro Kanayama
    • Ayumi Niwa
    • Kazuhisa Ishida
    • Takayuki Nakashima
    • Yuichiro Hatano
    • Natsuko Suzui
    • Tatsuhiko Miyazaki
    • Akira Hara
  • View Affiliations

  • Published online on: June 15, 2017     https://doi.org/10.3892/or.2017.5725
  • Pages: 775-784
  • Copyright: © Hisamatsu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Serrated lesions in the colorectum are currently predominantly classified as hyperplastic polyps (HPs), sessile serrated adenomas/polyps (SSA/Ps), and traditional serrated adenomas (TSAs) according to their morphology. However, the histological morphology and the molecular changes in the serrated lesions are still unclear. We performed immunohistochemistry for Ki67, p16INK4a, and WNT5A in human HPs (n=22), SSA/Ps (n=41), and TSAs (n=19). The distribution of Ki67 and p16INK4a positive cells in TSAs was different from that in HPs and SSA/Ps. Co-expression of Ki67 and P16INK4a was infrequent in HPs and SSA/Ps; p16INK4a-positive cells were found in the crypt cleft and stromal WNT5A-positive stromal cells were localized near the cleft in SSA/Ps, while intraepithelial lymphocytes (IELs) in SSA/Ps were more abundant than HPs. In conclusion, our study provides evidence that HPs branch because of the increase in and patchy distribution of senescent and proliferative cells, with increased and misdistributed stromal and inflammatory cells, which might contribute to creation of L- and/or T-shaped crypts, which are of distinctive shapes in SSA/Ps. Our findings may facilitate better understanding and therapy in the serrated lesions.
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August-2017
Volume 38 Issue 2

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Hisamatsu K, Noguchi K, Tomita H, Muto A, Yamada N, Kobayashi K, Hirata A, Kanayama T, Niwa A, Ishida K, Ishida K, et al: Distinctive crypt shape in a sessile serrated adenoma/polyp: Distribution of Ki67-, p16INK4a-, WNT5A-positive cells and intraepithelial lymphocytes. Oncol Rep 38: 775-784, 2017
APA
Hisamatsu, K., Noguchi, K., Tomita, H., Muto, A., Yamada, N., Kobayashi, K. ... Hara, A. (2017). Distinctive crypt shape in a sessile serrated adenoma/polyp: Distribution of Ki67-, p16INK4a-, WNT5A-positive cells and intraepithelial lymphocytes. Oncology Reports, 38, 775-784. https://doi.org/10.3892/or.2017.5725
MLA
Hisamatsu, K., Noguchi, K., Tomita, H., Muto, A., Yamada, N., Kobayashi, K., Hirata, A., Kanayama, T., Niwa, A., Ishida, K., Nakashima, T., Hatano, Y., Suzui, N., Miyazaki, T., Hara, A."Distinctive crypt shape in a sessile serrated adenoma/polyp: Distribution of Ki67-, p16INK4a-, WNT5A-positive cells and intraepithelial lymphocytes". Oncology Reports 38.2 (2017): 775-784.
Chicago
Hisamatsu, K., Noguchi, K., Tomita, H., Muto, A., Yamada, N., Kobayashi, K., Hirata, A., Kanayama, T., Niwa, A., Ishida, K., Nakashima, T., Hatano, Y., Suzui, N., Miyazaki, T., Hara, A."Distinctive crypt shape in a sessile serrated adenoma/polyp: Distribution of Ki67-, p16INK4a-, WNT5A-positive cells and intraepithelial lymphocytes". Oncology Reports 38, no. 2 (2017): 775-784. https://doi.org/10.3892/or.2017.5725