miR-30a inhibits glioma progression and stem cell‑like properties by repression of Wnt5a

Zhang,Yonghong; Wu,Zhi; Li,Lichao; Xie,Min

doi:10.3892/or.2017.5728

miR-30a inhibits glioma progression and stem cell‑like properties by repression of Wnt5a

Authors:
- Yonghong Zhang
- Zhi Wu
- Lichao Li
- Min Xie
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Affiliations: Department of Neurosurgery, The First Hospital of Lanzhou University, Lanzhou, Gansu 730000, P.R. China
Published online on: June 16, 2017 https://doi.org/10.3892/or.2017.5728
Pages: 1156-1162

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Abstract

miR-30a has been found to be dysregulated in diverse cancers and involved in the regulation of tumor progression. However, there is scarce research on the role of miR-30a in glioma. In the present study, we assessed the expression level of miR-30a in glioma tissues and cell lines. The microRNA microarray analysis revealed low expression of miR-30a in glioma tissues and cells vs. the control. Furthermore, we found that stable miR-30a inhibited cell proliferation, G1 phase arrest and stem cell-like formation in glioma. Moreover, to investigate the molecular mechanism of miR-30a on glioma cell phenotypes, we identified Wnt5a as a new direct target gene for miR-30a by bioinformatic assay, luciferase assay and western blot analysis. Further functional studies suggested that miR-30a suppressed metastasis, sphere formation and glioma growth by targeting Wnt5a signal pathway. Collectively, our findings suggested for the first time that miR-30a may function as a tumor suppressor in glioma by targeting Wnt5a.

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