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Print ISSN: 1021-335X Online ISSN: 1791-2431
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August-2017 Volume 38 Issue 2

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International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

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International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

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Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

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Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

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Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

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Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

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Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

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Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

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International Journal of Epigenetics

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Article

Lentivirus‑mediated knockdown of chondroitin polymerizing factor inhibits glioma cell growth in vitro

  • Authors:
    • Yang-Hua Fan
    • Bing Xiao
    • Shi-Gang Lv
    • Min-Hua Ye
    • Xin-Gen Zhu
    • Miao-Jing Wu
  • View Affiliations / Copyright

    Affiliations: Department of Neurosurgery, The Second Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330006, P.R. China
  • Pages: 1149-1155
    |
    Published online on: June 19, 2017
       https://doi.org/10.3892/or.2017.5731
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Abstract

Glioma is the most common primary tumor in the central nervous system, characterized by rapid progression, aggressive behavior, frequent recurrence and poor prognosis. In the present study we demonstrated that chondroitin polymerizing factor (CHPF) is highly expressed in human glioma tissues and 4 glioma cell lines. To explore the role of CHPF in glioma, a lentiviral vector expressing CHPF shRNA was constructed and transfected into the glioma U251 cells, which stably downregulated the expression levels of the CHPF gene in U251 cells in vitro. U251 cell proliferation inhibition rates were determined by MTT assay. The effect of survivin shRNA on U251 cell cycle distribution and cell apoptosis was determined by flow cytometry. Compared to the shRNA‑Ctrl group of cells, the shRNA-CHPF group of cells exhibited decreased proliferation and a significant increase in the proportion of cells in the G0/G1 phase. In addition, we found that knockdown of the expression of CHPF increased apoptosis in glioma U251 cells. Therefore, our results confirmed that CHPF promotes growth and inhibits apoptosis in glioma U251 cells. Thus, by in vivo and in vitro data, the present study suggests that CHPF could be a new potential therapeutic target for glioma.
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Copy and paste a formatted citation
Spandidos Publications style
Fan Y, Xiao B, Lv S, Ye M, Zhu X and Wu M: Lentivirus‑mediated knockdown of chondroitin polymerizing factor inhibits glioma cell growth in vitro. Oncol Rep 38: 1149-1155, 2017.
APA
Fan, Y., Xiao, B., Lv, S., Ye, M., Zhu, X., & Wu, M. (2017). Lentivirus‑mediated knockdown of chondroitin polymerizing factor inhibits glioma cell growth in vitro. Oncology Reports, 38, 1149-1155. https://doi.org/10.3892/or.2017.5731
MLA
Fan, Y., Xiao, B., Lv, S., Ye, M., Zhu, X., Wu, M."Lentivirus‑mediated knockdown of chondroitin polymerizing factor inhibits glioma cell growth in vitro". Oncology Reports 38.2 (2017): 1149-1155.
Chicago
Fan, Y., Xiao, B., Lv, S., Ye, M., Zhu, X., Wu, M."Lentivirus‑mediated knockdown of chondroitin polymerizing factor inhibits glioma cell growth in vitro". Oncology Reports 38, no. 2 (2017): 1149-1155. https://doi.org/10.3892/or.2017.5731
Copy and paste a formatted citation
x
Spandidos Publications style
Fan Y, Xiao B, Lv S, Ye M, Zhu X and Wu M: Lentivirus‑mediated knockdown of chondroitin polymerizing factor inhibits glioma cell growth in vitro. Oncol Rep 38: 1149-1155, 2017.
APA
Fan, Y., Xiao, B., Lv, S., Ye, M., Zhu, X., & Wu, M. (2017). Lentivirus‑mediated knockdown of chondroitin polymerizing factor inhibits glioma cell growth in vitro. Oncology Reports, 38, 1149-1155. https://doi.org/10.3892/or.2017.5731
MLA
Fan, Y., Xiao, B., Lv, S., Ye, M., Zhu, X., Wu, M."Lentivirus‑mediated knockdown of chondroitin polymerizing factor inhibits glioma cell growth in vitro". Oncology Reports 38.2 (2017): 1149-1155.
Chicago
Fan, Y., Xiao, B., Lv, S., Ye, M., Zhu, X., Wu, M."Lentivirus‑mediated knockdown of chondroitin polymerizing factor inhibits glioma cell growth in vitro". Oncology Reports 38, no. 2 (2017): 1149-1155. https://doi.org/10.3892/or.2017.5731
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