CIP2A, an oncoprotein, is associated with cell proliferation, invasion and migration in laryngeal carcinoma cells

  • Authors:
    • Xu-Dong Chen
    • Shi-Xiong Tang
    • Jian-Hua Zhang
    • Li-Tao Zhang
    • Yao-Wen Wang
  • View Affiliations

  • Published online on: June 27, 2017     https://doi.org/10.3892/or.2017.5759
  • Pages: 1005-1012
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Abstract

Laryngeal carcinoma is one of the most common malignant tumors in otorhinolaryngology. Moreover, experimental investigation showed that cancerous inhibitor of protein phosphatase 2A (CIP2A) expressed highly in various cancers. Therefore, we investigated whether CIP2A can regulate the proliferation, invasion and migration by RNA interference in Hep-2 cells and AMC-NH-8 cells and further affect the activation of phosphatidylinositol 3-kinase (PI3K)/protein kinase B (AKT) pathway. Overexpression of CIP2A was evaluated in tumor tissue and laryngeal cancer cell lines (Hep-2 and AMC-NH-8 cells) by real-time quantitative polymerase chain reaction (RT-qPCR) and western blot assay. In a follow-up experiment, we confirmed that CIP2A siRNA effectively suppressed the cell proliferation at 48 and 72 h, and arrested cell cycle at G0/G1 in Hep-2 cells and AMC-NH-8 cells. The invasion and migration of cell in siRNA CIP2A group were markedly inhibited. Moreover, the experimental results showed that the expression levels of invasion- and migration-related genes, including E-cadherin, metastasis-associated gene 1 (MTA1) and matrix metalloproteinases-2/9 (MMP-2/9), were regulated by CIP2A siRNA. Phosphorylation levels of PI3K and AKT proteins were reduced by CIP2A siRNA. Importantly, it suggested signaling through PI3K/Akt as a critical mechanism by which CIP2A siRNA may suppress cell proliferation, invasion and migration in laryngeal carcinoma cells.
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August-2017
Volume 38 Issue 2

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Chen X, Tang S, Zhang J, Zhang L and Wang Y: CIP2A, an oncoprotein, is associated with cell proliferation, invasion and migration in laryngeal carcinoma cells. Oncol Rep 38: 1005-1012, 2017
APA
Chen, X., Tang, S., Zhang, J., Zhang, L., & Wang, Y. (2017). CIP2A, an oncoprotein, is associated with cell proliferation, invasion and migration in laryngeal carcinoma cells. Oncology Reports, 38, 1005-1012. https://doi.org/10.3892/or.2017.5759
MLA
Chen, X., Tang, S., Zhang, J., Zhang, L., Wang, Y."CIP2A, an oncoprotein, is associated with cell proliferation, invasion and migration in laryngeal carcinoma cells". Oncology Reports 38.2 (2017): 1005-1012.
Chicago
Chen, X., Tang, S., Zhang, J., Zhang, L., Wang, Y."CIP2A, an oncoprotein, is associated with cell proliferation, invasion and migration in laryngeal carcinoma cells". Oncology Reports 38, no. 2 (2017): 1005-1012. https://doi.org/10.3892/or.2017.5759