MicroRNA-345 inhibits hepatocellular carcinoma metastasis by inhibiting YAP1

Zhang,Hui; Liu,Hui; Bi,Huaqiang

doi:10.3892/or.2017.5772

MicroRNA-345 inhibits hepatocellular carcinoma metastasis by inhibiting YAP1

Authors:
- Hui Zhang
- Hui Liu
- Huaqiang Bi
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Affiliations: Department of Radiology, Southwest Hospital, The Third Military Medical University, Chongqing 400038, P.R. China, Department of Hepatobiliary Surgery, Southwest Hospital, The Third Military Medical University, Chongqing 400038, P.R. China
Published online on: June 30, 2017 https://doi.org/10.3892/or.2017.5772
Pages: 843-849
Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

MicroRNAs (miRNAs) play critical roles in hepatocellular carcinoma (HCC). However, the expression and biological function of miR-345 in HCC remain unknown. The present study demonstrated that miR-345 expression was reduced in HCC tissues and cell lines. Decreased miR-345 expression was associated with unfavorable clinical features and poor prognosis. In vitro functional assays showed that miR-345 overexpression inhibited the migration and invasion of MHCC-97H cells while miR-345 knockdown promoted metastatic behavior of Hep3B cells. In vivo experiments showed that miR-345 overexpression inhibited while miR-345 knockdown promoted lung metastasis of HCC cells in nude mice. Mechanically, YAP1 was identified to be the downstream target of miR-345 in HCC cells. YAP1 overexpression reversed the inhibitory effects of miR-345 on MHCC-97H migration and invasion, while YAP1 knockdown reduced the promoting effects of miR-345 knockdown on the metastatic behavior of Hep3B cells.

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