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Article

Spica Prunellae extract suppresses the growth of human colon carcinoma cells by targeting multiple oncogenes via activating miR-34a

  • Authors:
    • Yi Fang
    • Ling Zhang
    • Jianyu Feng
    • Wei Lin
    • Qiaoyan Cai
    • Jun Peng
  • View Affiliations / Copyright

    Affiliations: Academy of Integrative Medicine and Fujian Key Laboratory of Integrative Medicine on Geriatrics, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350108, P.R. China
  • Pages: 1895-1901
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    Published online on: July 6, 2017
       https://doi.org/10.3892/or.2017.5792
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Abstract

Spica Prunellae is the spike of the herb Prunella vulgaris L. in traditional Chinese medicine which is often used for the treatment of various cancers including colorectal cancer. In the present study, we found that a key tumor suppressor, microRNA-34a (miR-34a) is involved in the antitumor activity for Spica Prunellae. Human colon carcinoma HCT-8 cells treated with an ethanol extract of Spica Prunellae (EESP) had significantly decreased cell proliferation and viability, in a dose-dependent manner. Flow cytometry analysis with Annexin V/PI staining analysis revealed that EESP treatment could induce apoptosis of HCT-8 cells. The level of miR-34a was upregulated in HCT-8 cells following EESP treatment, whereas expression levels of its target genes Notch1, Notch2 and Bcl-2 were downregulated. Inhibition of miR-34a rescued the expression of these target genes. These results revealed that Spica Prunellae can suppress the growth of HCT-8 cells by targeting Notch1, Notch2 and Bcl-2 via activation of miR-34a.
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Copy and paste a formatted citation
Spandidos Publications style
Fang Y, Zhang L, Feng J, Lin W, Cai Q and Peng J: Spica Prunellae extract suppresses the growth of human colon carcinoma cells by targeting multiple oncogenes via activating miR-34a. Oncol Rep 38: 1895-1901, 2017.
APA
Fang, Y., Zhang, L., Feng, J., Lin, W., Cai, Q., & Peng, J. (2017). Spica Prunellae extract suppresses the growth of human colon carcinoma cells by targeting multiple oncogenes via activating miR-34a. Oncology Reports, 38, 1895-1901. https://doi.org/10.3892/or.2017.5792
MLA
Fang, Y., Zhang, L., Feng, J., Lin, W., Cai, Q., Peng, J."Spica Prunellae extract suppresses the growth of human colon carcinoma cells by targeting multiple oncogenes via activating miR-34a". Oncology Reports 38.3 (2017): 1895-1901.
Chicago
Fang, Y., Zhang, L., Feng, J., Lin, W., Cai, Q., Peng, J."Spica Prunellae extract suppresses the growth of human colon carcinoma cells by targeting multiple oncogenes via activating miR-34a". Oncology Reports 38, no. 3 (2017): 1895-1901. https://doi.org/10.3892/or.2017.5792
Copy and paste a formatted citation
x
Spandidos Publications style
Fang Y, Zhang L, Feng J, Lin W, Cai Q and Peng J: Spica Prunellae extract suppresses the growth of human colon carcinoma cells by targeting multiple oncogenes via activating miR-34a. Oncol Rep 38: 1895-1901, 2017.
APA
Fang, Y., Zhang, L., Feng, J., Lin, W., Cai, Q., & Peng, J. (2017). Spica Prunellae extract suppresses the growth of human colon carcinoma cells by targeting multiple oncogenes via activating miR-34a. Oncology Reports, 38, 1895-1901. https://doi.org/10.3892/or.2017.5792
MLA
Fang, Y., Zhang, L., Feng, J., Lin, W., Cai, Q., Peng, J."Spica Prunellae extract suppresses the growth of human colon carcinoma cells by targeting multiple oncogenes via activating miR-34a". Oncology Reports 38.3 (2017): 1895-1901.
Chicago
Fang, Y., Zhang, L., Feng, J., Lin, W., Cai, Q., Peng, J."Spica Prunellae extract suppresses the growth of human colon carcinoma cells by targeting multiple oncogenes via activating miR-34a". Oncology Reports 38, no. 3 (2017): 1895-1901. https://doi.org/10.3892/or.2017.5792
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