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Article

Enhanced anticancer effects of a mixture of low-dose mushrooms and Panax ginseng root extracts in human colorectal cancer cells

  • Authors:
    • Mi So Lee
    • Mi-Sook Kim
    • Jae Kuk Yoo
    • Ji Young Lee
    • Jae Eun Ju
    • Youn Kyoung Jeong
  • View Affiliations / Copyright

    Affiliations: Radiation Non-clinical Center, Korea Institute of Radiological and Medical Sciences, Seoul 01812, Republic of Korea, Department of Radiation Oncology, Korea Institute of Radiological and Medical Sciences, Seoul 01812, Republic of Korea, Han Kook Shin Yak Pharmaceutical Co., Ltd., Nonsan 33023, Republic of Korea
  • Pages: 1597-1604
    |
    Published online on: July 7, 2017
       https://doi.org/10.3892/or.2017.5796
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Abstract

Worldwide, colorectal cancer is the third most common cancer in men and the second most common in women. As conventional colorectal cancer therapies result in various side effects, there is a need for adjuvant therapy that can enhance the conventional therapies without complications. In this study, we investigated the anticancer effects of combined mixture of the several medicinal mushrooms and Panax ginseng root extracts (also called Amex7) as an adjuvant compound in the treatment of human colorectal cancer. We observed the in vivo inhibitory effect of Amex7 (1.25, 6.25, and 12.5 ml/kg, oral administration, twice daily) on tumor growth in a mouse model xenografted with HT-29 human colorectal cancer cells. In vitro, at 6, 12, and 24 h after 4% Amex7 treatment, we analyzed cell cycle by flow cytometry and the expression levels of cell cycle progression, apoptosis, and DNA damage repair-related proteins using immunoblotting and immunofluorescence staining in HT-29 cell line. As a result, Amex7 significantly suppressed tumor growth in HT-29 human colorectal cancer cells and xenografts. In vitro, Amex7 induced G2/M arrest through the regulation of cell cycle proteins and cell death by apoptosis and autophagy. Additionally, Amex7 consistently induced DNA damage and delayed the repair of Amex7-induced DNA damage by reducing the level of HR repair proteins. In conclusion, Amex7 enhanced anticancer effects through the induction of G2/M arrest and cell death, including apoptosis and autophagy. Furthermore, Amex7 impaired DNA damage repair. The present study provides a scientific rationale for the clinical use of a combined mixture of medicinal mushrooms and P. ginseng root extracts as an adjuvant treatment in human colorectal cancer.
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1 

Ferlay J, Soerjomataram I, Dikshit R, Eser S, Mathers C, Rebelo M, Parkin DM, Forman D and Bray F: Cancer incidence and mortality worldwide: Sources, methods and major patterns in GLOBOCAN 2012. Int J Cancer. 136:E359–E386. 2015. View Article : Google Scholar : PubMed/NCBI

2 

Kim R: Treatment of Colorectal CancerCleveland Clinic Foundation. Lyndhurst, OH: 2010

3 

Safdie FM, Dorff T, Quinn D, Fontana L, Wei M, Lee C, Cohen P and Longo VD: Fasting and cancer treatment in humans: A case series report. Aging (Albany NY). 1:988–1007. 2009. View Article : Google Scholar : PubMed/NCBI

4 

Szakács G, Váradi A, Ozvegy-Laczka C and Sarkadi B: The role of ABC transporters in drug absorption, distribution, metabolism, excretion and toxicity (ADME-Tox). Drug Discov Today. 13:379–393. 2008. View Article : Google Scholar : PubMed/NCBI

5 

Wu CP, Ohnuma S and Ambudkar SV: Discovering natural product modulators to overcome multidrug resistance in cancer chemotherapy. Curr Pharm Biotechnol. 12:609–620. 2011. View Article : Google Scholar : PubMed/NCBI

6 

Loe DW, Deeley RG and Cole SP: Biology of the multidrug resistance-associated protein, MRP. Eur J Cancer. 32A:945–957. 1996. View Article : Google Scholar : PubMed/NCBI

7 

Penson RT, Oliva E, Skates SJ, Glyptis T, Fuller AF Jr, Goodman A and Seiden MV: Expression of multidrug resistance-1 protein inversely correlates with paclitaxel response and survival in ovarian cancer patients: A study in serial samples. Gynecol Oncol. 93:98–106. 2004. View Article : Google Scholar : PubMed/NCBI

8 

Belcaro G, Hosoi M, Pellegrini L, Appendino G, Ippolito E, Ricci A, Ledda A, Dugall M, Cesarone MR, Maione C, et al: A controlled study of a lecithinized delivery system of curcumin (Meriva®) to alleviate the adverse effects of cancer treatment. Phytother Res. 28:444–450. 2014. View Article : Google Scholar : PubMed/NCBI

9 

Kang HP, Lee H, Oh TG, Lee KJ, Park SJ, Chung MJ, Kim SU, Lee H, Park JC, Hong SP, et al: The use of health functional foods in gastrointestinal cancer patients. Clin Nutr Res. 2:19–25. 2013. View Article : Google Scholar : PubMed/NCBI

10 

Ballali S and Lanciai F: Functional food and diabetes: A natural way in diabetes prevention? Int J Food Sci Nutr. 63:(Suppl 1). 51–61. 2012. View Article : Google Scholar : PubMed/NCBI

11 

Alissa EM and Ferns GA: Functional foods and nutraceuticals in the primary prevention of cardiovascular diseases. J Nutr Metab. 2012:5694862012. View Article : Google Scholar : PubMed/NCBI

12 

Sliva D: Medicinal mushroom Phellinus linteus as an alternative cancer therapy. Exp Ther Med. 1:407–411. 2010.PubMed/NCBI

13 

Lee HJ, Lee HJ, Lim ES, Ahn KS, Shim BS, Kim HM, Gong SJ, Kim DK and Kim SH: Cambodian Phellinus linteus inhibits experimental metastasis of melanoma cells in mice via regulation of urokinase type plasminogen activator. Biol Pharm Bull. 28:27–31. 2005. View Article : Google Scholar : PubMed/NCBI

14 

Lee HS, Kim EJ and Kim SH: Ethanol extract of Inonotus obliquus (Chaga mushroom) induces G1 cell cycle arrest in HT-29 human colon cancer cells. Nutr Res Pract. 9:111–116. 2015. View Article : Google Scholar : PubMed/NCBI

15 

Guggenheim AG, Wright KM and Zwickey HL: Immune modulation from five major mushrooms: Application to integrative oncology. Integr Med (Encinitas). 13:32–44. 2014.PubMed/NCBI

16 

Park JG, Kang WS, Park KT, Park DJ, Aravinthan A, Kim JH and Cho JY: Anticancer effect of joboksansam, Korean wild ginseng germinated from bird feces. J Ginseng Res. 40:304–308. 2016. View Article : Google Scholar : PubMed/NCBI

17 

Yang Y, Yang WS, Yu T, Sung GH, Park KW, Yoon K, Son YJ, Hwang H, Kwak YS, Lee CM, et al: ATF-2/CREB/IRF-3-targeted anti-inflammatory activity of Korean red ginseng water extract. J Ethnopharmacol. 154:218–228. 2014. View Article : Google Scholar : PubMed/NCBI

18 

Lee CH and Kim JH: A review on the medicinal potentials of ginseng and ginsenosides on cardiovascular diseases. J Ginseng Res. 38:161–166. 2014. View Article : Google Scholar : PubMed/NCBI

19 

Elbatrawy EN, Ghonimy EA, Alassar MM and Wu FS: Medicinal mushroom extracts possess differential antioxidant activity and cytotoxicity to cancer cells. Int J Med Mushrooms. 17:471–479. 2015. View Article : Google Scholar : PubMed/NCBI

20 

Shin JY, Song JY, Yun YS, Yang HO, Rhee DK and Pyo S: Immunostimulating effects of acidic polysaccharides extract of Panax ginseng on macrophage function. Immunopharmacol Immunotoxicol. 24:469–482. 2002. View Article : Google Scholar : PubMed/NCBI

21 

Wasser SP and Weis AL: Therapeutic effects of substances occurring in higher Basidiomycetes mushrooms: A modern perspective. Crit Rev Immunol. 19:65–96. 1999.PubMed/NCBI

22 

Lee HH, Lee S, Lee K, Shin YS, Kang H and Cho H: Anti-cancer effect of Cordyceps militaris in human colorectal carcinoma RKO cells via cell cycle arrest and mitochondrial apoptosis. Daru. 23:352015. View Article : Google Scholar : PubMed/NCBI

23 

Song KS, Li G, Kim JS, Jing K, Kim TD, Kim JP, Seo SB, Yoo JK, Park HD, Hwang BD, et al: Protein-bound polysaccharide from Phellinus linteus inhibits tumor growth, invasion, and angiogenesis and alters Wnt/β-catenin in SW480 human colon cancer cells. BMC Cancer. 11:3072011. View Article : Google Scholar : PubMed/NCBI

24 

Park SE, Kim J, Lee YW, Yoo HS and Cho CK: Antitumor activity of water extracts from Cordyceps militaris in NCI-H460 cell xenografted nude mice. J Acupunct Meridian Stud. 2:294–300. 2009. View Article : Google Scholar : PubMed/NCBI

25 

Shangguan WJ, Li H and Zhang YH: Induction of G2/M phase cell cycle arrest and apoptosis by ginsenoside Rf in human osteosarcoma MG-63 cells through the mitochondrial pathway. Oncol Rep. 31:305–313. 2014.PubMed/NCBI

26 

Youn MJ, Kim JK, Park SY, Kim Y, Kim SJ, Lee JS, Chai KY, Kim HJ, Cui MX, So HS, et al: Chaga mushroom (Inonotus obliquus) induces G0/G1 arrest and apoptosis in human hepatoma HepG2 cells. World J Gastroenterol. 14:511–517. 2008. View Article : Google Scholar : PubMed/NCBI

27 

Li C, Tian ZN, Cai JP, Chen KX, Zhang B, Feng MY, Shi QT, Li R, Qin Y and Geng JS: Panax ginseng polysaccharide induces apoptosis by targeting Twist/AKR1C2/NF-1 pathway in human gastric cancer. Carbohydr Polym. 102:103–109. 2014. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Lee MS, Kim M, Yoo JK, Lee JY, Ju JE and Jeong YK: Enhanced anticancer effects of a mixture of low-dose mushrooms and Panax ginseng root extracts in human colorectal cancer cells. Oncol Rep 38: 1597-1604, 2017.
APA
Lee, M.S., Kim, M., Yoo, J.K., Lee, J.Y., Ju, J.E., & Jeong, Y.K. (2017). Enhanced anticancer effects of a mixture of low-dose mushrooms and Panax ginseng root extracts in human colorectal cancer cells. Oncology Reports, 38, 1597-1604. https://doi.org/10.3892/or.2017.5796
MLA
Lee, M. S., Kim, M., Yoo, J. K., Lee, J. Y., Ju, J. E., Jeong, Y. K."Enhanced anticancer effects of a mixture of low-dose mushrooms and Panax ginseng root extracts in human colorectal cancer cells". Oncology Reports 38.3 (2017): 1597-1604.
Chicago
Lee, M. S., Kim, M., Yoo, J. K., Lee, J. Y., Ju, J. E., Jeong, Y. K."Enhanced anticancer effects of a mixture of low-dose mushrooms and Panax ginseng root extracts in human colorectal cancer cells". Oncology Reports 38, no. 3 (2017): 1597-1604. https://doi.org/10.3892/or.2017.5796
Copy and paste a formatted citation
x
Spandidos Publications style
Lee MS, Kim M, Yoo JK, Lee JY, Ju JE and Jeong YK: Enhanced anticancer effects of a mixture of low-dose mushrooms and Panax ginseng root extracts in human colorectal cancer cells. Oncol Rep 38: 1597-1604, 2017.
APA
Lee, M.S., Kim, M., Yoo, J.K., Lee, J.Y., Ju, J.E., & Jeong, Y.K. (2017). Enhanced anticancer effects of a mixture of low-dose mushrooms and Panax ginseng root extracts in human colorectal cancer cells. Oncology Reports, 38, 1597-1604. https://doi.org/10.3892/or.2017.5796
MLA
Lee, M. S., Kim, M., Yoo, J. K., Lee, J. Y., Ju, J. E., Jeong, Y. K."Enhanced anticancer effects of a mixture of low-dose mushrooms and Panax ginseng root extracts in human colorectal cancer cells". Oncology Reports 38.3 (2017): 1597-1604.
Chicago
Lee, M. S., Kim, M., Yoo, J. K., Lee, J. Y., Ju, J. E., Jeong, Y. K."Enhanced anticancer effects of a mixture of low-dose mushrooms and Panax ginseng root extracts in human colorectal cancer cells". Oncology Reports 38, no. 3 (2017): 1597-1604. https://doi.org/10.3892/or.2017.5796
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