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Article

The differential regulation of microRNAs is associated with oral cancer

  • Authors:
    • Shih-Chang Tsai
    • Sheng-Fong Huang
    • Jo-Hua Chiang
    • Yen-Fu Chen
    • Chia-Chang Huang
    • Ming-Hsui Tsai
    • Fuu-Jen Tsai
    • Ming-Ching Kao
    • Jai-Sing Yang
  • View Affiliations / Copyright

    Affiliations: Department of Biological Science and Technology, China Medical University, Taichung, Taiwan, R.O.C., Department of Nursing, Chung-Jen Junior College of Nursing, Health Sciences and Management, Chiayi County, Taiwan, R.O.C., Department of Otolaryngology, China Medical University Hospital, Taichung, Taiwan, R.O.C., Human Genetic Center, China Medical University Hospital, Taichung, Taiwan, R.O.C., Department of Medical Research, China Medical University Hospital, China Medical University, Taichung, Taiwan, R.O.C.
  • Pages: 1613-1620
    |
    Published online on: July 12, 2017
       https://doi.org/10.3892/or.2017.5811
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Abstract

Oral squamous cell carcinoma (OSCC), is the most frequently occurring malignant head and neck tumor, generally it exhibits a poor prognosis, and metastasis is the main cause of death in these cancer patients. The discovery of reliable prognostic indicators for tumors progression would greatly improve clinical treatments. MicroRNAs (miRNAs) play a critical role in the degradation of mRNA and the inhibition of protein synthesis. The miRNAs function either as tumor suppressors or as oncogenes in tumorigenesis, and little is known about the clinical significance of miRNA expression profiles in oral cancers. In the present study, we investigated the expression profiles of miR-375, miR-204 and miR-196a in 39 healthy and tumor tissue pairs of oral cancer patients using TaqMan real-time quantitative polymerase chain reaction (qPCR). The predicted target genes for miR-375, miR-204 and miR-196a were confirmed using luciferase reporter-based assays and western blot analyses. In oral cancer tissue, the expression of miR-375 and miR-204 decreased, whereas the expression of miR-196a was significantly elevated. In OSCC, HOXB8 and p27 (CDKN1B) were the direct target genes of miR-196a, whereas HMGA2 was the direct target gene of miR-204. HOXB8 and p27 (CDKN1B) protein expression levels were inhibited by miR-196a, whereas the protein expression level of HMGA2 was inhibited by miR-204. Furthermore, the miR-196a inhibitor blocked cell proliferation. Our results indicate that the combined expression signatures of miR-375, miR-204 and miR-196a are promising biomarkers for the diagnosis, prognosis and treatment of OSCC.
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Copy and paste a formatted citation
Spandidos Publications style
Tsai S, Huang S, Chiang J, Chen Y, Huang C, Tsai M, Tsai F, Kao M and Yang J: The differential regulation of microRNAs is associated with oral cancer. Oncol Rep 38: 1613-1620, 2017.
APA
Tsai, S., Huang, S., Chiang, J., Chen, Y., Huang, C., Tsai, M. ... Yang, J. (2017). The differential regulation of microRNAs is associated with oral cancer. Oncology Reports, 38, 1613-1620. https://doi.org/10.3892/or.2017.5811
MLA
Tsai, S., Huang, S., Chiang, J., Chen, Y., Huang, C., Tsai, M., Tsai, F., Kao, M., Yang, J."The differential regulation of microRNAs is associated with oral cancer". Oncology Reports 38.3 (2017): 1613-1620.
Chicago
Tsai, S., Huang, S., Chiang, J., Chen, Y., Huang, C., Tsai, M., Tsai, F., Kao, M., Yang, J."The differential regulation of microRNAs is associated with oral cancer". Oncology Reports 38, no. 3 (2017): 1613-1620. https://doi.org/10.3892/or.2017.5811
Copy and paste a formatted citation
x
Spandidos Publications style
Tsai S, Huang S, Chiang J, Chen Y, Huang C, Tsai M, Tsai F, Kao M and Yang J: The differential regulation of microRNAs is associated with oral cancer. Oncol Rep 38: 1613-1620, 2017.
APA
Tsai, S., Huang, S., Chiang, J., Chen, Y., Huang, C., Tsai, M. ... Yang, J. (2017). The differential regulation of microRNAs is associated with oral cancer. Oncology Reports, 38, 1613-1620. https://doi.org/10.3892/or.2017.5811
MLA
Tsai, S., Huang, S., Chiang, J., Chen, Y., Huang, C., Tsai, M., Tsai, F., Kao, M., Yang, J."The differential regulation of microRNAs is associated with oral cancer". Oncology Reports 38.3 (2017): 1613-1620.
Chicago
Tsai, S., Huang, S., Chiang, J., Chen, Y., Huang, C., Tsai, M., Tsai, F., Kao, M., Yang, J."The differential regulation of microRNAs is associated with oral cancer". Oncology Reports 38, no. 3 (2017): 1613-1620. https://doi.org/10.3892/or.2017.5811
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