Clinical significance of miR-138 in patients with malignant melanoma through targeting of PDK1 in the PI3K/AKT autophagy signaling pathway

  • Authors:
    • Fanjun Meng
    • Yuxia Zhang
    • Xing Li
    • Juan Wang
    • Zhiyu Wang
  • View Affiliations

  • Published online on: July 19, 2017     https://doi.org/10.3892/or.2017.5838
  • Pages: 1655-1662
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Abstract

The present study investigated the clinical significance of miR-138 in patients with malignant melanoma (MM), which has previously been associated with tumor growth. In patients with MM, we found that the expression of miR-138 was significantly downregulated when compared with healthy control subjects. Overexpression of miR-138 in the human melanoma cell line A2058 inhibited cell proliferation and induced cell apoptosis, and increased caspase-3 and Bax protein expression when compared with a negative control group. Meanwhile, miR-138 overexpression promoted cell autophagy, induced LC3 protein expression, and suppressed the PI3K/AKT/mTOR signaling pathway and PDK1 protein expression in A2058 cells. LY294002, an inhibitor of PI3K, suppressed PI3K/AKT/mTOR signaling, induced apoptosis, inhibited cell proliferation, increased caspase-3 and Bax protein expression, and decreased PDK1 protein expression in A2058 cells following miR-138 overexpression. Collectively, our findings indicate the clinical significance of miR-138 in patients with MM through its targeting of PDK1 expression in the PI3K/AKT/mTOR autophagy signaling pathway.
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September-2017
Volume 38 Issue 3

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Meng F, Zhang Y, Li X, Wang J and Wang Z: Clinical significance of miR-138 in patients with malignant melanoma through targeting of PDK1 in the PI3K/AKT autophagy signaling pathway. Oncol Rep 38: 1655-1662, 2017
APA
Meng, F., Zhang, Y., Li, X., Wang, J., & Wang, Z. (2017). Clinical significance of miR-138 in patients with malignant melanoma through targeting of PDK1 in the PI3K/AKT autophagy signaling pathway. Oncology Reports, 38, 1655-1662. https://doi.org/10.3892/or.2017.5838
MLA
Meng, F., Zhang, Y., Li, X., Wang, J., Wang, Z."Clinical significance of miR-138 in patients with malignant melanoma through targeting of PDK1 in the PI3K/AKT autophagy signaling pathway". Oncology Reports 38.3 (2017): 1655-1662.
Chicago
Meng, F., Zhang, Y., Li, X., Wang, J., Wang, Z."Clinical significance of miR-138 in patients with malignant melanoma through targeting of PDK1 in the PI3K/AKT autophagy signaling pathway". Oncology Reports 38, no. 3 (2017): 1655-1662. https://doi.org/10.3892/or.2017.5838