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Article

Heat shock-induced HIKESHI protects cell viability via nuclear translocation of heat shock protein 70

  • Authors:
    • Toru Yanoma
    • Kyoichi Ogata
    • Takehiko Yokobori
    • Munenori Ide
    • Erito Mochiki
    • Yoshitaka Toyomasu
    • Mitsuhiro Yanai
    • Norimichi Kogure
    • Akiharu Kimura
    • Masaki Suzuki
    • Nobuhiro Nakazawa
    • Tuya Bai
    • Tetsunari Oyama
    • Takayuki Asao
    • Ken Shirabe
    • Hiroyuki Kuwano
  • View Affiliations / Copyright

    Affiliations: Department of General Surgical Science, Gunma University Graduate School of Medicine, Maebashi, Gunma, Japan, Department of Diagnostic Pathology, Gunma University Graduate School of Medicine, Maebashi, Gunma, Japan, Department of Digestive Tract and General Surgery, Saitama Medical Center, Saitama Medical University, Saitama, Japan, Big Data Center for Integrative Analysis, Gunma University Initiative for Advance Research (GIAR), Maebashi, Gunma, Japan, Department of Hepatobiliary and Pancreatic Surgery, Gunma University Graduate School of Medicine, Maebashi, Gunma, Japan
  • Pages: 1500-1506
    |
    Published online on: July 21, 2017
       https://doi.org/10.3892/or.2017.5844
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Abstract

Heat shock proteins (HSPs), particularly HSP70, help restore normal cellular function following damage caused by stressors. HSP expression in tumor tissues indicates cancer progression, and while the development of HSP inhibitors is progressing, these substances are not widely used to treat cancer. HIKESHI (C11orf73) does not control the intracellular movement of HSP70 at normal temperatures; however, it does regulate the function and movements of HSP70 during heat shock. In this study, we examined the intracellular movement of HSP70 during heat shock to investigate the significance of HIKESHI expression in gastric cancer (GC) and determine if HIKESHI inhibition has cytotoxic effects. We examined HIKESHI using GC cell lines and immunostaining in 207 GC tissue samples. HIKESHI expression in GC tissues was associated with the progression of lymphatic invasion. Suppressing HIKESHI using siRNA did not affect cell viability at normal temperatures. However, suppressing HIKESHI during heat shock inhibited HSP70 nuclear transport and suppressed cell viability. Our results suggest that HIKESHI is a marker of cancer progression and that the combination of HIKESHI inhibition and hyperthermia is a therapeutic tool for refractory GC.
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1 

Paoletti X, Oba K, Burzykowski T, Michiels S, Ohashi Y, Pignon JP, Rougier P, Sakamoto J, Sargent D, Sasako M, et al: GASTRIC (Global Advanced/Adjuvant Stomach Tumor Research International Collaboration) Group: Benefit of adjuvant chemotherapy for resectable gastric cancer: A meta-analysis. JAMA. 303:1729–1737. 2010. View Article : Google Scholar : PubMed/NCBI

2 

Shen L, Shan YS, Hu HM, Price TJ, Sirohi B, Yeh KH, Yang YH, Sano T, Yang HK, Zhang X, et al: Management of gastric cancer in Asia: Resource-stratified guidelines. Lancet Oncol. 14:e535–e547. 2013. View Article : Google Scholar : PubMed/NCBI

3 

Kanda M and Kodera Y: Molecular mechanisms of peritoneal dissemination in gastric cancer. World J Gastroenterol. 22:6829–6840. 2016. View Article : Google Scholar : PubMed/NCBI

4 

Mochiki E, Shioya M, Sakurai H, Andoh H, Ohno T, Aihara R, Asao T and Kuwano H: Feasibility study of postoperative intraperitoneal hyperthermochemotherapy by radiofrequency capacitive heating system for advanced gastric cancer with peritoneal seeding. Int J Hyperther. 23:493–500. 2007. View Article : Google Scholar

5 

Gidalevitz T, Prahlad V and Morimoto RI: The stress of protein misfolding: From single cells to multicellular organisms. Cold Spring Harb Perspect Biol. 3:32011. View Article : Google Scholar

6 

Hartl FU, Bracher A and Hayer-Hartl M: Molecular chaperones in protein folding and proteostasis. Nature. 475:324–332. 2011. View Article : Google Scholar : PubMed/NCBI

7 

Richter K, Haslbeck M and Buchner J: The heat shock response: Life on the verge of death. Mol Cell. 40:253–266. 2010. View Article : Google Scholar : PubMed/NCBI

8 

Furuta M, Kose S, Koike M, Shimi T, Hiraoka Y, Yoneda Y, Haraguchi T and Imamoto N: Heat-shock induced nuclear retention and recycling inhibition of importin alpha. Genes Cells. 9:429–441. 2004. View Article : Google Scholar : PubMed/NCBI

9 

Subjeck JR and Shyy TT: Stress protein systems of mammalian cells. Am J Physiol. 250:C1–C17. 1986.PubMed/NCBI

10 

Wang XY and Subjeck JR: High molecular weight stress proteins: Identification, cloning and utilisation in cancer immunotherapy. Int J Hyperther. 29:364–375. 2013. View Article : Google Scholar

11 

Ciocca DR and Calderwood SK: Heat shock proteins in cancer: Diagnostic, prognostic, predictive, and treatment implications. Cell Stress Chaperones. 10:86–103. 2005. View Article : Google Scholar : PubMed/NCBI

12 

Kimura A, Ogata K, Altan B, Yokobori T, Ide M, Mochiki E, Toyomasu Y, Kogure N, Yanoma T, Suzuki M, et al: Nuclear heat shock protein 110 expression is associated with poor prognosis and chemotherapy resistance in gastric cancer. Oncotarget. 7:18415–18423. 2016. View Article : Google Scholar : PubMed/NCBI

13 

Nanbu K, Konishi I, Komatsu T, Mandai M, Yamamoto S, Kuroda H, Koshiyama M and Mori T: Expression of heat shock proteins HSP70 and HSP90 in endometrial carcinomas. Correlation with clinicopathology, sex steroid receptor status, and p53 protein expression. Cancer. 77:330–338. 1996. View Article : Google Scholar : PubMed/NCBI

14 

Lazaris AC, Chatzigianni EB, Panoussopoulos D, Tzimas GN, Davaris PS and Golematis BC: Proliferating cell nuclear antigen and heat shock protein 70 immunolocalization in invasive ductal breast cancer not otherwise specified. Breast Cancer Res Treat. 43:43–51. 1997. View Article : Google Scholar : PubMed/NCBI

15 

Fang X, Jiang Y, Feng L, Chen H, Zhen C, Ding M and Wang X: Blockade of PI3K/AKT pathway enhances sensitivity of Raji cells to chemotherapy through down-regulation of HSP70. Cancer Cell Int. 13:482013. View Article : Google Scholar : PubMed/NCBI

16 

He LF, Guan KP, Yan Z, Ye HY, Xu KX, Ren L and Hou SK: Enhanced sensitivity to mitomycin C by abating heat shock protein 70 expression in human bladder cancer cell line of BIU-87. Chin Med J (Engl). 118:1965–1972. 2005.PubMed/NCBI

17 

Wang Y, Koay YC and McAlpine SR: How selective are Hsp90 inhibitors for cancer cells over normal cells? ChemMedChem. 12:353–357. 2017. View Article : Google Scholar : PubMed/NCBI

18 

Johnson ML, Yu HA, Hart EM, Weitner BB, Rademaker AW, Patel JD, Kris MG and Riely GJ: Phase I/II study of HSP90 inhibitor AUY922 and erlotinib for EGFR-mutant lung cancer with acquired resistance to epidermal growth factor receptor tyrosine kinase inhibitors. J Clin Oncol. 33:1666–1673. 2015. View Article : Google Scholar : PubMed/NCBI

19 

Kose S, Furuta M and Imamoto N: Hikeshi, a nuclear import carrier for Hsp70s, protects cells from heat shock-induced nuclear damage. Cell. 149:578–589. 2012. View Article : Google Scholar : PubMed/NCBI

20 

Pelham HR: Hsp70 accelerates the recovery of nucleolar morphology after heat shock. EMBO J. 3:3095–3100. 1984.PubMed/NCBI

21 

Velazquez JM and Lindquist S: hsp70: Nuclear concentration during environmental stress and cytoplasmic storage during recovery. Cell. 36:655–662. 1984. View Article : Google Scholar : PubMed/NCBI

22 

Welch WJ and Feramisco JR: Nuclear and nucleolar localization of the 72,000-dalton heat shock protein in heat-shocked mammalian cells. J Biol Chem. 259:4501–4513. 1984.PubMed/NCBI

23 

Imamoto N and Kose S: Heat-shock stress activates a novel nuclear import pathway mediated by Hikeshi. Nucleus. 3:422–428. 2012. View Article : Google Scholar : PubMed/NCBI

24 

Japanese Gastric Cancer Association: Japanese classification of gastric carcinoma: 3rd English edition. Gastric Cancer. 14:101–112. 2011. View Article : Google Scholar : PubMed/NCBI

25 

Bodoor K, Jalboush SA, Matalka I, Abu-Sheikha A, Waq RA, Ebwaini H, Abu-Awad A, Fayyad L, Al-Arjat J and Haddad Y: Heat shock protein association with clinico-pathological characteristics of gastric cancer in Jordan: HSP70 is predictive of poor prognosis. Asian Pac J Cancer Prev. 17:3929–3937. 2016.PubMed/NCBI

26 

Lee HW, Lee EH, Kim SH, Roh MS, Jung SB and Choi YC: Heat shock protein 70 (HSP70) expression is associated with poor prognosis in intestinal type gastric cancer. Virchows Arch. 463:489–495. 2013. View Article : Google Scholar : PubMed/NCBI

27 

Stewart M: Molecular mechanism of the nuclear protein import cycle. Nat Rev Mol Cell Biol. 8:195–208. 2007. View Article : Google Scholar : PubMed/NCBI

28 

Kose S and Imamoto N: Nucleocytoplasmic transport under stress conditions and its role in HSP70 chaperone systems. Biochim Biophys Acta. 1840:2953–2960. 2014. View Article : Google Scholar : PubMed/NCBI

29 

Chant ID, Rose PE and Morris AG: Analysis of heat-shock protein expression in myeloid leukaemia cells by flow cytometry. Br J Haematol. 90:163–168. 1995. View Article : Google Scholar : PubMed/NCBI

30 

Xiang TX, Li Y, Jiang Z, Huang AL, Luo C, Zhan B, Wang PL and Tao XH: RNA interference-mediated silencing of the Hsp70 gene inhibits human gastric cancer cell growth and induces apoptosis in vitro and in vivo. Tumori. 94:539–550. 2008.PubMed/NCBI

31 

Liu J, Liu J, Guo SY, Liu HL and Li SZ: HSP70 inhibitor combined with cisplatin suppresses the cervical cancer proliferation in vitro and transplanted tumor growth: An experimental study. Asian Pac J Trop Med. 10:184–188. 2017. View Article : Google Scholar : PubMed/NCBI

32 

Court KA, Hatakeyama H, Wu SY, Lingegowda MS, Rodríguez-Aguayo C, López-Berestein G, Ju-Seog L, Rinaldi C, Juan EJ, Sood AK, et al: HSP70 inhibition synergistically enhances the effects of magnetic fluid hyperthermia in ovarian cancer. Mol Cancer Ther. 16:966–976. 2017. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Yanoma T, Ogata K, Yokobori T, Ide M, Mochiki E, Toyomasu Y, Yanai M, Kogure N, Kimura A, Suzuki M, Suzuki M, et al: Heat shock-induced HIKESHI protects cell viability via nuclear translocation of heat shock protein 70 . Oncol Rep 38: 1500-1506, 2017.
APA
Yanoma, T., Ogata, K., Yokobori, T., Ide, M., Mochiki, E., Toyomasu, Y. ... Kuwano, H. (2017). Heat shock-induced HIKESHI protects cell viability via nuclear translocation of heat shock protein 70 . Oncology Reports, 38, 1500-1506. https://doi.org/10.3892/or.2017.5844
MLA
Yanoma, T., Ogata, K., Yokobori, T., Ide, M., Mochiki, E., Toyomasu, Y., Yanai, M., Kogure, N., Kimura, A., Suzuki, M., Nakazawa, N., Bai, T., Oyama, T., Asao, T., Shirabe, K., Kuwano, H."Heat shock-induced HIKESHI protects cell viability via nuclear translocation of heat shock protein 70 ". Oncology Reports 38.3 (2017): 1500-1506.
Chicago
Yanoma, T., Ogata, K., Yokobori, T., Ide, M., Mochiki, E., Toyomasu, Y., Yanai, M., Kogure, N., Kimura, A., Suzuki, M., Nakazawa, N., Bai, T., Oyama, T., Asao, T., Shirabe, K., Kuwano, H."Heat shock-induced HIKESHI protects cell viability via nuclear translocation of heat shock protein 70 ". Oncology Reports 38, no. 3 (2017): 1500-1506. https://doi.org/10.3892/or.2017.5844
Copy and paste a formatted citation
x
Spandidos Publications style
Yanoma T, Ogata K, Yokobori T, Ide M, Mochiki E, Toyomasu Y, Yanai M, Kogure N, Kimura A, Suzuki M, Suzuki M, et al: Heat shock-induced HIKESHI protects cell viability via nuclear translocation of heat shock protein 70 . Oncol Rep 38: 1500-1506, 2017.
APA
Yanoma, T., Ogata, K., Yokobori, T., Ide, M., Mochiki, E., Toyomasu, Y. ... Kuwano, H. (2017). Heat shock-induced HIKESHI protects cell viability via nuclear translocation of heat shock protein 70 . Oncology Reports, 38, 1500-1506. https://doi.org/10.3892/or.2017.5844
MLA
Yanoma, T., Ogata, K., Yokobori, T., Ide, M., Mochiki, E., Toyomasu, Y., Yanai, M., Kogure, N., Kimura, A., Suzuki, M., Nakazawa, N., Bai, T., Oyama, T., Asao, T., Shirabe, K., Kuwano, H."Heat shock-induced HIKESHI protects cell viability via nuclear translocation of heat shock protein 70 ". Oncology Reports 38.3 (2017): 1500-1506.
Chicago
Yanoma, T., Ogata, K., Yokobori, T., Ide, M., Mochiki, E., Toyomasu, Y., Yanai, M., Kogure, N., Kimura, A., Suzuki, M., Nakazawa, N., Bai, T., Oyama, T., Asao, T., Shirabe, K., Kuwano, H."Heat shock-induced HIKESHI protects cell viability via nuclear translocation of heat shock protein 70 ". Oncology Reports 38, no. 3 (2017): 1500-1506. https://doi.org/10.3892/or.2017.5844
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