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Article

B7-H3 is related to tumor progression in ovarian cancer

  • Authors:
    • Jingjing Zhang
    • Lu Liu
    • Sai Han
    • Yi Li
    • Qiuhong Qian
    • Qianqian Zhang
    • Hui Zhang
    • Ziyan Yang
    • Youzhong Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Obstetrics and Gynecology, Qilu Hospital of Shandong University, Jinan, Shandong, P.R. China, Department of Obstetrics and Gynecology, The Affiliated Hospital of Taishan Medical College, Taian City, Shandong, P.R. China
  • Pages: 2426-2434
    |
    Published online on: July 31, 2017
       https://doi.org/10.3892/or.2017.5858
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Abstract

B7-H3, a co-stimulatory molecule, has been found expressed in ovarian cancer, but its role and mechanism is not clear. In this study, we further verified the expression of B7-H3 in ovarian carcinoma and normal epithelial ovarian tissues. Three ovarian cancer cell lines, A2780, SKOV3 and HO8910 were selected to explore the effects of B7-H3 on proliferation, apoptosis, migration and invasion. We found that B7-H3 was mainly located in the cytoplasm of ovarian cancer cells as determined by immunofluorescence staining. The ability of cell invasion, migration, proliferation decreased after silencing B7-H3 whereas the apoptosis increased, which was related to the upregulation of Bax, caspase-8, cleaved caspase-8 and the downregulation of Bcl-2, Bcl-xl, matrix metalloproteinase-2 (MMP2) by western blotting. In addition, B7-H3 enhanced the H08910 cell capacities in invasion, migration and proliferation. Expression of the phosphorylation signal transducer and activator of transcription 3 (pStat3) molecules and their upstream molecules phosphorylation Janus kinase 2 (pJak2) were significantly increased. In order to investigate whether B7-H3 plays a role in this pathway, we treated the overexpressed HO8910 cells with AG490 (inhibitors of Jak2). Our findings revealed that B7-H3 affect ovarian cancer progression through the Jak2/Stat3 pathway, indicating that B7-H3 has the potential to be a useful prognostic marker.
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Copy and paste a formatted citation
Spandidos Publications style
Zhang J, Liu L, Han S, Li Y, Qian Q, Zhang Q, Zhang H, Yang Z and Zhang Y: B7-H3 is related to tumor progression in ovarian cancer. Oncol Rep 38: 2426-2434, 2017.
APA
Zhang, J., Liu, L., Han, S., Li, Y., Qian, Q., Zhang, Q. ... Zhang, Y. (2017). B7-H3 is related to tumor progression in ovarian cancer. Oncology Reports, 38, 2426-2434. https://doi.org/10.3892/or.2017.5858
MLA
Zhang, J., Liu, L., Han, S., Li, Y., Qian, Q., Zhang, Q., Zhang, H., Yang, Z., Zhang, Y."B7-H3 is related to tumor progression in ovarian cancer". Oncology Reports 38.4 (2017): 2426-2434.
Chicago
Zhang, J., Liu, L., Han, S., Li, Y., Qian, Q., Zhang, Q., Zhang, H., Yang, Z., Zhang, Y."B7-H3 is related to tumor progression in ovarian cancer". Oncology Reports 38, no. 4 (2017): 2426-2434. https://doi.org/10.3892/or.2017.5858
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang J, Liu L, Han S, Li Y, Qian Q, Zhang Q, Zhang H, Yang Z and Zhang Y: B7-H3 is related to tumor progression in ovarian cancer. Oncol Rep 38: 2426-2434, 2017.
APA
Zhang, J., Liu, L., Han, S., Li, Y., Qian, Q., Zhang, Q. ... Zhang, Y. (2017). B7-H3 is related to tumor progression in ovarian cancer. Oncology Reports, 38, 2426-2434. https://doi.org/10.3892/or.2017.5858
MLA
Zhang, J., Liu, L., Han, S., Li, Y., Qian, Q., Zhang, Q., Zhang, H., Yang, Z., Zhang, Y."B7-H3 is related to tumor progression in ovarian cancer". Oncology Reports 38.4 (2017): 2426-2434.
Chicago
Zhang, J., Liu, L., Han, S., Li, Y., Qian, Q., Zhang, Q., Zhang, H., Yang, Z., Zhang, Y."B7-H3 is related to tumor progression in ovarian cancer". Oncology Reports 38, no. 4 (2017): 2426-2434. https://doi.org/10.3892/or.2017.5858
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