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Article

Wild-type p53 controls the level of fibronectin expression in breast cancer cells

  • Authors:
    • Daeun You
    • Seung Pil Jung
    • Yisun Jeong
    • Soo Youn Bae
    • Sangmin Kim
  • View Affiliations / Copyright

    Affiliations: Department of Health Sciences and Technology, SAIHST, Sungkyunkwan University, Gangnam-gu, Seoul 06351, Republic of Korea, Division of Breast and Endocrine Surgery, Department of Surgery, Korea University Hospital, Korea University College of Medicine, Seongbuk-gu, Seoul 02852, Republic of Korea, Department of Breast Cancer Center, Samsung Medical Center, Gangnam-gu, Seoul 06351, Republic of Korea
  • Pages: 2551-2557
    |
    Published online on: July 31, 2017
       https://doi.org/10.3892/or.2017.5860
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Abstract

Aberrant fibronectin (FN) expression is associated with poor prognosis, cell adhesion, and cell motility in a variety of cancer cells. In this study, we investigated the relationship between p53 and FN expression in breast cancer cells. Basal FN expression was significantly decreased by treatment with the p53 activator III, RITA, in MCF7 breast cancer cells with wild-type p53. In addition, overexpression of wild-type p53 markedly decreased the level of FN expression in p53-mutant breast cancer cells. To examine the mechanism underlying the relationship between p53 and FN expression, we treated MCF7 breast cancer cells with the tumor promoter TPA (12-O-tetradecanoylphorbol-13-acetate). Our results showed that basal FN expression was increased by TPA treatment in a time-dependent manner. In contrast, the level of p53 expression was decreased by TPA treatment. However, the expression of FN and p53 was not altered by TPA in p53-mutant breast cancer cells. Furthermore, the alterations in FN and p53 expression in response to TPA were prevented by a specific MEK inhibitor, UO126. Finally, we demonstrated that TPA triggers degradation of p53 through the proteasomal pathway in MCF7 cells. TPA-induced FN expression was decreased by the proteasome inhibitor MG132. Under the same condition, p53 protein expression, but not mRNA expression, was reversed by MG132. Taken together, our data demonstrate that the level of FN expression is associated with the status and expression of p53 in breast cancer cells.
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Copy and paste a formatted citation
Spandidos Publications style
You D, Jung SP, Jeong Y, Bae SY and Kim S: Wild-type p53 controls the level of fibronectin expression in breast cancer cells. Oncol Rep 38: 2551-2557, 2017.
APA
You, D., Jung, S.P., Jeong, Y., Bae, S.Y., & Kim, S. (2017). Wild-type p53 controls the level of fibronectin expression in breast cancer cells. Oncology Reports, 38, 2551-2557. https://doi.org/10.3892/or.2017.5860
MLA
You, D., Jung, S. P., Jeong, Y., Bae, S. Y., Kim, S."Wild-type p53 controls the level of fibronectin expression in breast cancer cells". Oncology Reports 38.4 (2017): 2551-2557.
Chicago
You, D., Jung, S. P., Jeong, Y., Bae, S. Y., Kim, S."Wild-type p53 controls the level of fibronectin expression in breast cancer cells". Oncology Reports 38, no. 4 (2017): 2551-2557. https://doi.org/10.3892/or.2017.5860
Copy and paste a formatted citation
x
Spandidos Publications style
You D, Jung SP, Jeong Y, Bae SY and Kim S: Wild-type p53 controls the level of fibronectin expression in breast cancer cells. Oncol Rep 38: 2551-2557, 2017.
APA
You, D., Jung, S.P., Jeong, Y., Bae, S.Y., & Kim, S. (2017). Wild-type p53 controls the level of fibronectin expression in breast cancer cells. Oncology Reports, 38, 2551-2557. https://doi.org/10.3892/or.2017.5860
MLA
You, D., Jung, S. P., Jeong, Y., Bae, S. Y., Kim, S."Wild-type p53 controls the level of fibronectin expression in breast cancer cells". Oncology Reports 38.4 (2017): 2551-2557.
Chicago
You, D., Jung, S. P., Jeong, Y., Bae, S. Y., Kim, S."Wild-type p53 controls the level of fibronectin expression in breast cancer cells". Oncology Reports 38, no. 4 (2017): 2551-2557. https://doi.org/10.3892/or.2017.5860
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